rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Free-floating mesothelial cells in pleural fluid after lung surgery
  name:
    name part: Kienzle, Arne Udo
    name part: 1992-
    role:
      role term: creator
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Servais, Andrew B.
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Ysasi, Alexandra B.
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Gibney, Barry C.
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Valenzuela, Cristian D.
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Wagner, Willi Linus
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Ackermann, Maximilian
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Mentzer, Steven J.
    role:
      role term: VerfasserIn
    role:
      role term: aut
  type of resource: text
  origin info:
    place:
      place term: xx
    date issued: 2018
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 8
  abstract: Objectives: The mesothelium, the surface layer of the heart, lung, bowel, liver and tunica vaginalis, is a complex tissue implicated in organ-specific diseases and regenerative biology; however, the mechanism of mesothelial repair after surgical injury is unknown. Previous observations indicated seeding of denuded mesothelium by free-floating mesothelial cells may contribute to mesothelial healing. In this study, we investigated the prevalence of mesothelial cells in pleural fluid during the 7 days following pulmonary surgery. Study design: Flow cytometry was employed to study pleural fluid of 45 patients after lung resection or transplantation. We used histologically validated mesothelial markers (CD71 and WT1) to estimate the prevalence of mesothelial cells. Results: The viability of pleural fluid cells approached 100%. Leukocytes and mesothelial cells were identified in the pleural fluid within the first week after surgery. The leukocyte concentration was relatively stable at all time points. In contrast, mesothelial cells, identified by CD71 and WT1 peaked on POD3. The broad expression of CD71 molecule in postoperative pleural fluid suggests that many of the free-floating non-leukocyte cells were activated or proliferative mesothelial cells. Conclusion: We demonstrated that pleural fluid post lung surgery is a source of mesothelial cells; most of these cells appear to be viable and, as shown by CD71 staining, activated mesothelial cells. The observed peak of mesothelial cells on POD3 is consistent with a potential reparative role of free-floating mesothelial cells after pulmonary surgery.
  note: Arne Kienzle, Andrew B. Servais, Alexandra B. Ysasi, Barry C. Gibney, Cristian D. Valenzuela, Willi L. Wagner, Maximilian Ackermann and Steven J. Mentzer
  note: Gesehen am 21.10.2019
  subject:
    topic: lung healing
  subject:
    topic: Lung regeneration
  subject:
    topic: mesothelial cells
  subject:
    topic: Pleural fluid
  subject:
    topic: Pneumonectomy
  classification: 33
  related item:
    title info:
      title: Frontiers in medicine
    name:
      name part: Free-floating mesothelial cells in pleural fluid after lung surgery
    origin info:
      publisher: Lausanne : Frontiers Media, 2014
    physical description:
      extent: Online-Ressource
    identifier: 2296-858X
    identifier: (DE-627)789482991
    identifier: (DE-600)2775999-4
    identifier: (DE-576)408729597
    part:
      text: 5(2018), Artikel-ID 89, Seite 1-8
  related item:
    name:
      name part: Free-floating mesothelial cells in pleural fluid after lung surgery
    part:
      text: volume:5
    part:
      text: year:2018
    part:
      text: elocationid:89
    part:
      text: pages:1-8
    part:
      text: extent:8
  identifier: https://doi.org/10.3389/fmed.2018.00089
  identifier: https://www.frontiersin.org/articles/10.3389/fmed.2018.00089/full
  record info:
    record content source: DE-627
    record creation date: 191021
    record change date: 20230615081844.0
    record identifier: 167923935X
    language of cataloging:
      language term: ger