Combined immunohistochemistry after mass spectrometry imaging for superior spatial information
Objective Tissue slides analyzed by MS imaging (MSI) are stained by H&E (Haematoxylin and Eosin) to identify regions of interest. As it can be difficult to identify specific cells of interest by H&E alone, data analysis may be impaired. Immunohistochemistry (IHC) can highlight cells of inter...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2019
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| In: |
Proteomics. Clinical applications
Year: 2018, Jahrgang: 13, Heft: 1 |
| ISSN: | 1862-8354 |
| DOI: | 10.1002/prca.201800035 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1002/prca.201800035 Verlag: https://onlinelibrary.wiley.com/doi/abs/10.1002/prca.201800035 
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| Verfasserangaben: | Katharina Kriegsmann, Rémi Longuespée, Michael Hundemer, Christiane Zgorzelski, Rita Casadonte, Kristina Schwamborn, Wilko Weichert, Peter Schirmacher, Alexander Harms, Daniel Kazdal, Jonas Leichsenring, Albrecht Stenzinger, Arne Warth, Margaux Fresnais, Jörg Kriegsmann, and Mark Kriegsmann |
MARC
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| 245 | 1 | 0 | |a Combined immunohistochemistry after mass spectrometry imaging for superior spatial information |c Katharina Kriegsmann, Rémi Longuespée, Michael Hundemer, Christiane Zgorzelski, Rita Casadonte, Kristina Schwamborn, Wilko Weichert, Peter Schirmacher, Alexander Harms, Daniel Kazdal, Jonas Leichsenring, Albrecht Stenzinger, Arne Warth, Margaux Fresnais, Jörg Kriegsmann, and Mark Kriegsmann |
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| 520 | |a Objective Tissue slides analyzed by MS imaging (MSI) are stained by H&E (Haematoxylin and Eosin) to identify regions of interest. As it can be difficult to identify specific cells of interest by H&E alone, data analysis may be impaired. Immunohistochemistry (IHC) can highlight cells of interest but single or combined IHC on tissue sections analyzed by MSI have not been performed. Methods We performed MSI on bone marrow biopsies from patients with multiple myeloma and stained different antibodies (CD38, CD138, MUM1, kappa- and lambda). A combination of CK5/6/TTF1 and Napsin-A/p40 is stained after MSI on adenocarcinoma and squamous cell carcinoma of the lung. Staining intensities of p40 after MSI and on a serial section are quantified on a tissue microarray (n = 44) by digital analysis. Results Digital evaluation reveals weaker staining intensities after MSI as compared to serial sections. Staining quality and quantity after MSI enables to identify cells of interest. On the tissue microarray, one out of 44 tissue specimens shows no staining of p40 after MSI, but weak nuclear staining on a serial section. Conclusion We demonstrated that single and double IHC staining is feasible on tissue sections previously analyzed by MSI, with decreased staining intensities. | ||
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