Metabolomic analysis of Shiga toxin 2a-induced injury in conditionally immortalized glomerular endothelial cells
IntroductionShiga toxin 2a (Stx2a) induces hemolytic uremic syndrome (STEC HUS) by targeting glomerular endothelial cells (GEC).ObjectivesWe investigated in a metabolomic analysis the response of a conditionally immortalized, stable glomerular endothelial cell line (ciGEnC) to Stx2a stimulation as a...
Gespeichert in:
| Hauptverfasser: | , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
01 October 2019
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| In: |
Metabolomics
Year: 2019, Jahrgang: 15, Heft: 10 |
| ISSN: | 1573-3890 |
| DOI: | 10.1007/s11306-019-1594-2 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1007/s11306-019-1594-2
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| Verfasserangaben: | Christian Patry, Kathrin Plotnicki, Christian Betzen, Alba Perez Ortiz, Kirk L. Pappan, Simon C. Satchell, Peter W. Mathieson, Martina Bielaszewska, Helge Karch, Burkhard Tönshoff, Neysan Rafat |
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| 245 | 1 | 0 | |a Metabolomic analysis of Shiga toxin 2a-induced injury in conditionally immortalized glomerular endothelial cells |c Christian Patry, Kathrin Plotnicki, Christian Betzen, Alba Perez Ortiz, Kirk L. Pappan, Simon C. Satchell, Peter W. Mathieson, Martina Bielaszewska, Helge Karch, Burkhard Tönshoff, Neysan Rafat |
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| 520 | |a IntroductionShiga toxin 2a (Stx2a) induces hemolytic uremic syndrome (STEC HUS) by targeting glomerular endothelial cells (GEC).ObjectivesWe investigated in a metabolomic analysis the response of a conditionally immortalized, stable glomerular endothelial cell line (ciGEnC) to Stx2a stimulation as a cell culture model for STEC HUS.MethodsCiGEnC were treated with tumor necrosis factor-(TNF)α, Stx2a or sequentially with TNFα and Stx2a. We performed a metabolomic high-throughput screening by lipid- or gas chromatography and subsequent mass spectrometry. Metabolite fold changes in stimulated ciGEnC compared to untreated cells were calculated.Results320 metabolites were identified and investigated. In response to TNFα + Stx2a, there was a predominant increase in intracellular free fatty acids and amino acids. Furthermore, lipid- and protein derived pro-inflammatory mediators, oxidative stress and an augmented intracellular energy turnover were increased in ciGEnC. Levels of most biochemicals related to carbohydrate metabolism remained unchanged.ConclusionStimulation of ciGEnC with TNFα + Stx2a is associated with profound metabolic changes indicative of increased inflammation, oxidative stress and energy turnover. | ||
| 650 | 4 | |a Conditionally immortalized glomerular endothelial cells | |
| 650 | 4 | |a Hemolytic uremic syndrome | |
| 650 | 4 | |a Metabolomics | |
| 650 | 4 | |a Shiga toxin | |
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