Development and validation of HIV-1 Multiplex Serology
By inducing immunosuppression in infected patients, human immunodeficiency virus-1 (HIV-1) generates a favorable environment for opportunistic infections and the development of several human cancers. In order to detect individual serum or plasma HIV-1 antibody status for epidemiological studies, hig...
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| Hauptverfasser: | , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
18 January 2019
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| In: |
Journal of immunological methods
Year: 2019, Jahrgang: 466, Pages: 47-51 |
| ISSN: | 1872-7905 |
| DOI: | 10.1016/j.jim.2019.01.007 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1016/j.jim.2019.01.007 Verlag: https://linkinghub.elsevier.com/retrieve/pii/S0022175918303521 
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| Verfasserangaben: | L.M. Kranz, B. Gärtner, A. Michel, M. Pawlita, T. Waterboer, N. Brenner |
| Zusammenfassung: | By inducing immunosuppression in infected patients, human immunodeficiency virus-1 (HIV-1) generates a favorable environment for opportunistic infections and the development of several human cancers. In order to detect individual serum or plasma HIV-1 antibody status for epidemiological studies, high-throughput HIV-1 Multiplex Serology was developed. Seven HIV-1 antigens were recombinantly expressed in E. coli as N-terminal glutathione-S-transferase (GST) fusion proteins that are bound to glutathione-coupled sets of beads with distinct fluorescent color. Combining all bead sets in a suspension array allowed for simultaneous detection of antibodies targeting structural, regulatory and accessory proteins expressed during HIV-1 infection. HIV-1 Multiplex Serology was validated with 244 reference sera whose HIV-1 serostatus had been pre-determined by screening microparticle immunoassay and confirmatory line immunoassay. The multifunctional protein GAG emerged as an excellent marker to determine HIV-1 serostatus with a specificity of 99% (95% CI 96%-100%) and sensitivity of 100% (95% CI 88%-100%). Seropositivity for multiple HIV-1 antigens appeared to be characteristic for HIV-1 infected individuals (median number of antigens recognized in reference assay positive sera: 4; median number of antigens recognized in reference assay negative sera: 0), indicating a broad immune response targeting also regulatory and accessory proteins which may be useful for the identification of antibody patterns specific for infection-associated disease stages. HIV-1 Multiplex Serology performs similarly to conventional HIV-1 serology but eliminates the need for a two-step screening approach with subsequent confirmation assay. Thus, this highthroughput method will facilitate large-scale epidemiological studies of the role of HIV-1 in cancer development. |
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| Beschreibung: | Gesehen am 29.10.2019 |
| Beschreibung: | Online Resource |
| ISSN: | 1872-7905 |
| DOI: | 10.1016/j.jim.2019.01.007 |