The Escherichia coli SRP receptor forms a homodimer at the membrane
The Escherichia coli signal recognition particle (SRP) receptor, FtsY, plays a fundamental role in co-translational targeting of membrane proteins via the SRP pathway. Efficient targeting relies on membrane interaction of FtsY and heterodimerization with the SRP protein Ffh, which is driven by detac...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
August 23, 2018
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| In: |
Structure
Year: 2018, Jahrgang: 26, Heft: 11, Pages: 1440-1450.e5 |
| ISSN: | 1878-4186 |
| DOI: | 10.1016/j.str.2018.07.008 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1016/j.str.2018.07.008 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0969212618302545 
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| Verfasserangaben: | Georg Kempf, Goran Stjepanovic, Jeremy Sloan, Astrid Hendricks, Karine Lapouge, and Irmgard Sinning |
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| 520 | |a The Escherichia coli signal recognition particle (SRP) receptor, FtsY, plays a fundamental role in co-translational targeting of membrane proteins via the SRP pathway. Efficient targeting relies on membrane interaction of FtsY and heterodimerization with the SRP protein Ffh, which is driven by detachment of α helix (αN1) in FtsY. Here we show that apart from the heterodimer, FtsY forms a nucleotide-dependent homodimer on the membrane, and upon αN1 removal also in solution. Homodimerization triggers reciprocal stimulation of GTP hydrolysis and occurs in vivo. Biochemical characterization together with integrative modeling suggests that the homodimer employs the same interface as the heterodimer. Structure determination of FtsY NG+1 with GMPPNP shows that a dimerization-induced conformational switch of the γ-phosphate is conserved in Escherichia coli, filling an important gap in SRP GTPase activation. Our findings add to the current understanding of SRP GTPases and may challenge previous studies that did not consider homodimerization of FtsY. | ||
| 650 | 4 | |a co-translational protein targeting | |
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| 650 | 4 | |a MTS | |
| 650 | 4 | |a SAXS | |
| 650 | 4 | |a signal recognition particle | |
| 650 | 4 | |a SRP | |
| 650 | 4 | |a SRP GTPases | |
| 650 | 4 | |a SRP receptor | |
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