Plate-based large-scale cultivation of Caenorhabditis elegans: sample preparation for the study of metabolic alterations in diabetes
Culturing Caenorhabditis elegans (C. elegans) in a large-scale manner on agar plates can be time-consuming and difficult. This protocol describes a simple and inexpensive method to obtain a large number of animals for the isolation of proteins to proceed with a western blot, mass spectrometry, or fu...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) Video |
| Sprache: | Englisch |
| Veröffentlicht: |
8/24/2018
|
| In: |
JoVE. Video journal
Year: 2018, Heft: 138, Pages: 1-8 |
| ISSN: | 1940-087X |
| DOI: | 10.3791/58117 |
| Schlagworte: | |
| Online-Zugang: | Resolving-System, Volltext: https://doi.org/10.3791/58117 Verlag: https://www.jove.com/video/58117/plate-based-large-scale-cultivation-caenorhabditis-elegans-sample 
|
| Verfasserangaben: | Katharina Kohl, Thomas Fleming, Kübra Acunman, Hans-Peter Hammes, Michael Morcos, Andrea Schlotterer |
| Zusammenfassung: | Culturing Caenorhabditis elegans (C. elegans) in a large-scale manner on agar plates can be time-consuming and difficult. This protocol describes a simple and inexpensive method to obtain a large number of animals for the isolation of proteins to proceed with a western blot, mass spectrometry, or further proteomics analyses. Furthermore, an increase of nematode numbers for immunostainings and the integration of multiple analyses under the same culturing conditions can easily be achieved. Additionally, a transfer between plates with different experimental conditions is facilitated. |
|---|---|
| Beschreibung: | Enthält auch eine Versuchsbeschreibung in Textform Gesehen am 05.12.2019 Wissenschaftlicher Film. Deutschland. 2018 |
| Beschreibung: | Online Resource |
| ISSN: | 1940-087X |
| DOI: | 10.3791/58117 |