High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl4-induced hepatic damage
Oxidative stress plays a crucial role in the development of several liver diseases. Many natural polyphenols can attenuate oxidative stress and liver injury. In this study, a phytochemical profiling of a methanol extract from leaves of Syzygium samarangense revealed 92 compounds belonging to flavono...
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| Hauptverfasser: | , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
31 January 2018
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| In: |
Food and chemical toxicology
Year: 2018, Jahrgang: 113, Pages: 145-153 |
| ISSN: | 1873-6351 |
| DOI: | 10.1016/j.fct.2018.01.031 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1016/j.fct.2018.01.031 Verlag: http://www.sciencedirect.com/science/article/pii/S0278691518300310 
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| Verfasserangaben: | Mansour Sobeh, Fadia S. Youssef, Ahmed Esmat, Ganna Petruk, Ahmed H. El-Khatib, Daria Maria Monti, Mohamed L. Ashour, Michael Wink |
| Zusammenfassung: | Oxidative stress plays a crucial role in the development of several liver diseases. Many natural polyphenols can attenuate oxidative stress and liver injury. In this study, a phytochemical profiling of a methanol extract from leaves of Syzygium samarangense revealed 92 compounds belonging to flavonoids, phenolic acids, condensed tannins, and ellagitannins. The S. samarangense extract exhibited a noticeable antioxidant activity with an EC50 of 5.80μg/mL measured by DPPH scavenging capacity assay, 2632 Trolox equivalents, 10mM Fe2+ equivalents/mg of samples by TEAC and FRAP assays, respectively. The total phenolic content was 419mg gallic acid equivalent GAE/g extract. In a cell-based model (HaCaT cells), the extract completely inhibited ROS production induced by UVA, and prevented GSH-depletion and p38 phosphorylation. In addition, the extract exhibited a substantial antioxidant and hepatoprotective activities in CCl4-treated rats, with an increase in GSH (reduced glutathione) and SOD (superoxide dismutase) activities by 84.75 and 26.27%, respectively, and a decrease of 19.08, 63.05, 52.21, 37.00, 13.26, and 15.15% in MDA, ALT, AST, TB (total bilirubin), TC (total cholesterol), and TG (total glycerides), respectively. These results were confirmed by histopathological analyses. We believe that Syzygium samarangense is a good candidate for further evaluation as an antioxidant and liver protecting drug. |
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| Beschreibung: | Gesehen am 17.12.2019 Im Titel ist die Zahl 4 tiefgestellt dargestellt Syzygium samarangense ist kursiv dargestellt |
| Beschreibung: | Online Resource |
| ISSN: | 1873-6351 |
| DOI: | 10.1016/j.fct.2018.01.031 |