Biallelic variants in ASNA1, encoding a cytosolic targeting factor of tail-anchored proteins, cause rapidly progressive pediatric cardiomyopathy
Background:Pediatric cardiomyopathies are a clinically and genetically heterogeneous group of heart muscle disorders associated with high morbidity and mortality. Although knowledge of the genetic basis of pediatric cardiomyopathy has improved considerably, the underlying cause remains elusive in a...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
28 Aug 2019
|
| In: |
Circulation. Genomic and precision medicine
Year: 2019, Jahrgang: 12, Heft: 9, Pages: 397-406 |
| ISSN: | 2574-8300 |
| DOI: | 10.1161/CIRCGEN.119.002507 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1161/CIRCGEN.119.002507 Verlag, Volltext: https://www.ahajournals.org/doi/full/10.1161/CIRCGEN.119.002507 
|
| Verfasserangaben: | Judith M.A. Verhagen, Myrthe van den Born, Herma C. van der Linde, Peter G.J. Nikkels, Rob M. Verdijk, Maryann H. Kivlen, Leontine M.A. van Unen, Annette F. Baas, Henriette ter Heide, Lennie van Osch-Gevers, Marianne Hoogeveen-Westerveld, Johanna C. Herkert, Aida M. Bertoli-Avella, Marjon A. van Slegtenhorst, Marja W. Wessels, Frans W. Verheijen, David Hassel, Robert M.W. Hofstra, Ramanujan S. Hegde, Peter M. van Hasselt, Tjakko J. van Ham Tjakko, Ingrid M.B.H. van de Laar |
MARC
| LEADER | 00000caa a22000002c 4500 | ||
|---|---|---|---|
| 001 | 1686326289 | ||
| 003 | DE-627 | ||
| 005 | 20220817195151.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 191223s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1161/CIRCGEN.119.002507 |2 doi | |
| 035 | |a (DE-627)1686326289 | ||
| 035 | |a (DE-599)KXP1686326289 | ||
| 035 | |a (OCoLC)1341286758 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Verhagen, Judith M. A. |e VerfasserIn |0 (DE-588)1202022170 |0 (DE-627)1686326637 |4 aut | |
| 245 | 1 | 0 | |a Biallelic variants in ASNA1, encoding a cytosolic targeting factor of tail-anchored proteins, cause rapidly progressive pediatric cardiomyopathy |c Judith M.A. Verhagen, Myrthe van den Born, Herma C. van der Linde, Peter G.J. Nikkels, Rob M. Verdijk, Maryann H. Kivlen, Leontine M.A. van Unen, Annette F. Baas, Henriette ter Heide, Lennie van Osch-Gevers, Marianne Hoogeveen-Westerveld, Johanna C. Herkert, Aida M. Bertoli-Avella, Marjon A. van Slegtenhorst, Marja W. Wessels, Frans W. Verheijen, David Hassel, Robert M.W. Hofstra, Ramanujan S. Hegde, Peter M. van Hasselt, Tjakko J. van Ham Tjakko, Ingrid M.B.H. van de Laar |
| 264 | 1 | |c 28 Aug 2019 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 23.12.2019 | ||
| 520 | |a Background:Pediatric cardiomyopathies are a clinically and genetically heterogeneous group of heart muscle disorders associated with high morbidity and mortality. Although knowledge of the genetic basis of pediatric cardiomyopathy has improved considerably, the underlying cause remains elusive in a substantial proportion of cases.Methods:Exome sequencing was used to screen for the causative genetic defect in a pair of siblings with rapidly progressive dilated cardiomyopathy and death in early infancy. Protein expression was assessed in patient samples, followed by an in vitro tail-anchored protein insertion assay and functional analyses in zebrafish.Results:We identified compound heterozygous variants in the highly conserved ASNA1 gene (arsA arsenite transporter, ATP-binding, homolog), which encodes an ATPase required for post-translational membrane insertion of tail-anchored proteins. The c.913C>T variant on the paternal allele is predicted to result in a premature stop codon p.(Gln305*), and likely explains the decreased protein expression observed in myocardial tissue and skin fibroblasts. The c.488T>C variant on the maternal allele results in a valine to alanine substitution at residue 163 (p.Val163Ala). Functional studies showed that this variant leads to protein misfolding as well as less effective tail-anchored protein insertion. Loss of asna1 in zebrafish resulted in reduced cardiac contractility and early lethality. In contrast to wild-type mRNA, injection of either mutant mRNA failed to rescue this phenotype.Conclusions:Biallelic variants in ASNA1 cause severe pediatric cardiomyopathy and early death. Our findings point toward a critical role of the tail-anchored membrane protein insertion pathway in vertebrate cardiac function and disease. | ||
| 700 | 1 | |a Hassel, David |e VerfasserIn |0 (DE-588)135801613 |0 (DE-627)693785608 |0 (DE-576)300653808 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Circulation. Genomic and precision medicine |d Philadelphia, Pa. : Lippincott, Williams & Wilkins, 2018 |g 12(2019), 9, Seite 397-406 |h Online-Ressource |w (DE-627)1019378298 |w (DE-600)2927603-2 |w (DE-576)502270454 |x 2574-8300 |7 nnas |a Biallelic variants in ASNA1, encoding a cytosolic targeting factor of tail-anchored proteins, cause rapidly progressive pediatric cardiomyopathy |
| 773 | 1 | 8 | |g volume:12 |g year:2019 |g number:9 |g pages:397-406 |g extent:10 |a Biallelic variants in ASNA1, encoding a cytosolic targeting factor of tail-anchored proteins, cause rapidly progressive pediatric cardiomyopathy |
| 856 | 4 | 0 | |u https://doi.org/10.1161/CIRCGEN.119.002507 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://www.ahajournals.org/doi/full/10.1161/CIRCGEN.119.002507 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20191223 | ||
| 993 | |a Article | ||
| 994 | |a 2019 | ||
| 998 | |g 135801613 |a Hassel, David |m 135801613:Hassel, David |d 50000 |e 50000PH135801613 |k 0/50000/ |p 17 | ||
| 999 | |a KXP-PPN1686326289 |e 357031068X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title":"Biallelic variants in ASNA1, encoding a cytosolic targeting factor of tail-anchored proteins, cause rapidly progressive pediatric cardiomyopathy","title_sort":"Biallelic variants in ASNA1, encoding a cytosolic targeting factor of tail-anchored proteins, cause rapidly progressive pediatric cardiomyopathy"}],"person":[{"family":"Verhagen","given":"Judith M. A.","roleDisplay":"VerfasserIn","display":"Verhagen, Judith M. A.","role":"aut"},{"display":"Hassel, David","roleDisplay":"VerfasserIn","role":"aut","family":"Hassel","given":"David"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 23.12.2019"],"recId":"1686326289","language":["eng"],"origin":[{"dateIssuedDisp":"28 Aug 2019","dateIssuedKey":"2019"}],"id":{"doi":["10.1161/CIRCGEN.119.002507"],"eki":["1686326289"]},"name":{"displayForm":["Judith M.A. Verhagen, Myrthe van den Born, Herma C. van der Linde, Peter G.J. Nikkels, Rob M. Verdijk, Maryann H. Kivlen, Leontine M.A. van Unen, Annette F. Baas, Henriette ter Heide, Lennie van Osch-Gevers, Marianne Hoogeveen-Westerveld, Johanna C. Herkert, Aida M. Bertoli-Avella, Marjon A. van Slegtenhorst, Marja W. Wessels, Frans W. Verheijen, David Hassel, Robert M.W. Hofstra, Ramanujan S. Hegde, Peter M. van Hasselt, Tjakko J. van Ham Tjakko, Ingrid M.B.H. van de Laar"]},"physDesc":[{"extent":"10 S."}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"id":{"issn":["2574-8300"],"eki":["1019378298"],"zdb":["2927603-2"]},"origin":[{"publisher":"Lippincott, Williams & Wilkins","dateIssuedDisp":"January 16, 2018","publisherPlace":"Philadelphia, Pa."}],"name":{"displayForm":["American Heart Association"]},"part":{"pages":"397-406","issue":"9","year":"2019","extent":"10","volume":"12","text":"12(2019), 9, Seite 397-406"},"titleAlt":[{"title":"Circ Genom Precis Med"}],"pubHistory":["Volume 11 (January 2018)-"],"language":["eng"],"corporate":[{"role":"isb","roleDisplay":"Herausgebendes Organ","display":"American Heart Association"}],"recId":"1019378298","disp":"Biallelic variants in ASNA1, encoding a cytosolic targeting factor of tail-anchored proteins, cause rapidly progressive pediatric cardiomyopathyCirculation. Genomic and precision medicine","type":{"media":"Online-Ressource","bibl":"periodical"},"title":[{"partname":"Genomic and precision medicine","title_sort":"Circulation","title":"Circulation"}]}]} | ||
| SRT | |a VERHAGENJUBIALLELICV2820 | ||