Molecular imaging of fibroblast activity after myocardial infarction using a 68Ga-labeled fibroblast activation protein inhibitor, FAPI-04
Heart failure remains a major source of late morbidity and mortality after myocardial infarction (MI). The temporospatial presence of activated fibroblasts in the injured myocardium predicts the quality of cardiac remodeling after MI. Therefore, monitoring of activated fibroblasts is of great intere...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
August 12, 2019
|
| In: |
Journal of nuclear medicine
Year: 2019, Jahrgang: 60, Heft: 12, Pages: 1743-1749 |
| ISSN: | 2159-662X |
| DOI: | 10.2967/jnumed.119.226993 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.2967/jnumed.119.226993 Verlag, Volltext: http://jnm.snmjournals.org/content/60/12/1743 
|
| Verfasserangaben: | Zohreh Varasteh, Sarajo Mohanta, Stephanie Robu, Miriam Braeuer, Yuanfang Li, Negar Omidvari, Geoffrey Topping, Ting Sun, Stephan G. Nekolla, Antonia Richter, Christian Weber, Andreas Habenicht, Uwe A. Haberkorn, and Wolfgang A. Weber |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1687645442 | ||
| 003 | DE-627 | ||
| 005 | 20230427215206.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200117s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.2967/jnumed.119.226993 |2 doi | |
| 035 | |a (DE-627)1687645442 | ||
| 035 | |a (DE-599)KXP1687645442 | ||
| 035 | |a (OCoLC)1341298376 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Varasteh, Zohreh |e VerfasserIn |0 (DE-588)120299198X |0 (DE-627)1687649391 |4 aut | |
| 245 | 1 | 0 | |a Molecular imaging of fibroblast activity after myocardial infarction using a 68Ga-labeled fibroblast activation protein inhibitor, FAPI-04 |c Zohreh Varasteh, Sarajo Mohanta, Stephanie Robu, Miriam Braeuer, Yuanfang Li, Negar Omidvari, Geoffrey Topping, Ting Sun, Stephan G. Nekolla, Antonia Richter, Christian Weber, Andreas Habenicht, Uwe A. Haberkorn, and Wolfgang A. Weber |
| 264 | 1 | |c August 12, 2019 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 17.01.2019 | ||
| 500 | |a Im Titel ist "68" in 68Ga-labeled hochgestellt | ||
| 520 | |a Heart failure remains a major source of late morbidity and mortality after myocardial infarction (MI). The temporospatial presence of activated fibroblasts in the injured myocardium predicts the quality of cardiac remodeling after MI. Therefore, monitoring of activated fibroblasts is of great interest for studying cardiac remodeling after MI. Fibroblast activation protein (FAP) expression is upregulated in activated fibroblasts. This study investigated the feasibility of imaging activated fibroblasts with a new 68Ga-labeled FAP inhibitor (68Ga-FAPI-04) for PET imaging of fibroblast activation in a preclinical model of MI. Methods: MI and sham-operated rats were scanned with 68Ga-FAPI-04 PET/CT (1, 3, 6, 14, 23, and 30 d after MI) and with 18F-FDG (3 d after MI). Dynamic 68Ga-FAPI-04 PET and blocking studies were performed on MI rats 7 d after coronary ligation. After in vivo scans, the animals were euthanized and their hearts harvested for ex vivo analyses. Cryosections were prepared for autoradiography, hematoxylin and eosin (H&E), and immunofluorescence staining. Results: 68Ga-FAPI-04 uptake in the injured myocardium peaked on day 6 after coronary ligation. The tracer accumulated intensely in the MI territory, as identified by decreased 18F-FDG uptake and confirmed by PET/MR and H&E staining. Autoradiography and H&E staining of cross-sections revealed that 68Ga-FAPI-04 accumulated mainly at the border zone of the infarcted myocardium. In contrast, there was only minimal uptake in the infarct of the blocked rats, comparable to the uptake in the remote noninfarcted myocardium (PET image-derived ratio of infarct uptake to remote uptake: 6 ± 2). Immunofluorescence staining confirmed the presence of FAP-positive myofibroblasts in the injured myocardium. Morphometric analysis of the whole-heart sections demonstrated 3- and 8-fold higher FAP-positive fibroblast density in the border zone than in the infarct center and remote area, respectively. Conclusion: 68Ga-FAPI-04 represents a promising radiotracer for in vivo imaging of post-MI fibroblast activation. Noninvasive imaging of activated fibroblasts may have significant diagnostic and prognostic value, which could aid clinical management of patients after MI. | ||
| 650 | 4 | |a cardiac remodeling | |
| 650 | 4 | |a fibroblast activation protein | |
| 650 | 4 | |a molecular imaging | |
| 650 | 4 | |a myocardial infarction | |
| 650 | 4 | |a PET | |
| 700 | 1 | |a Mohanta, Sarajo |e VerfasserIn |4 aut | |
| 700 | 1 | |a Robu, Stephanie |e VerfasserIn |4 aut | |
| 700 | 1 | |a Braeuer, Miriam |e VerfasserIn |4 aut | |
| 700 | 1 | |a Li, Yuanfang |e VerfasserIn |4 aut | |
| 700 | 1 | |a Omidvari, Negar |e VerfasserIn |4 aut | |
| 700 | 1 | |a Topping, Geoffrey |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sun, Ting |e VerfasserIn |4 aut | |
| 700 | 1 | |a Nekolla, Stephan G. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Richter, Antonia |e VerfasserIn |4 aut | |
| 700 | 1 | |a Weber, Christian |e VerfasserIn |4 aut | |
| 700 | 1 | |a Habenicht, Andreas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Haberkorn, Uwe |d 1959- |e VerfasserIn |0 (DE-588)1022913905 |0 (DE-627)717331245 |0 (DE-576)366166352 |4 aut | |
| 700 | 1 | |a Weber, Wolfgang A. |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of nuclear medicine |d New York, NY : Soc., 1964 |g 60(2019), 12, Seite 1743-1749 |h Online-Ressource |w (DE-627)325793603 |w (DE-600)2040222-3 |w (DE-576)09475277X |x 2159-662X |7 nnas |a Molecular imaging of fibroblast activity after myocardial infarction using a 68Ga-labeled fibroblast activation protein inhibitor, FAPI-04 |
| 773 | 1 | 8 | |g volume:60 |g year:2019 |g number:12 |g pages:1743-1749 |g extent:7 |a Molecular imaging of fibroblast activity after myocardial infarction using a 68Ga-labeled fibroblast activation protein inhibitor, FAPI-04 |
| 856 | 4 | 0 | |u https://doi.org/10.2967/jnumed.119.226993 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://jnm.snmjournals.org/content/60/12/1743 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200117 | ||
| 993 | |a Article | ||
| 994 | |a 2019 | ||
| 998 | |g 1022913905 |a Haberkorn, Uwe |m 1022913905:Haberkorn, Uwe |d 910000 |e 910000PH1022913905 |k 0/910000/ |p 13 | ||
| 999 | |a KXP-PPN1687645442 |e 357617513X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"family":"Varasteh","role":"aut","given":"Zohreh","display":"Varasteh, Zohreh"},{"family":"Mohanta","role":"aut","given":"Sarajo","display":"Mohanta, Sarajo"},{"given":"Stephanie","display":"Robu, Stephanie","family":"Robu","role":"aut"},{"display":"Braeuer, Miriam","given":"Miriam","role":"aut","family":"Braeuer"},{"given":"Yuanfang","display":"Li, Yuanfang","family":"Li","role":"aut"},{"display":"Omidvari, Negar","given":"Negar","family":"Omidvari","role":"aut"},{"family":"Topping","role":"aut","given":"Geoffrey","display":"Topping, Geoffrey"},{"given":"Ting","display":"Sun, Ting","role":"aut","family":"Sun"},{"family":"Nekolla","role":"aut","given":"Stephan G.","display":"Nekolla, Stephan G."},{"given":"Antonia","display":"Richter, Antonia","role":"aut","family":"Richter"},{"role":"aut","family":"Weber","given":"Christian","display":"Weber, Christian"},{"display":"Habenicht, Andreas","given":"Andreas","family":"Habenicht","role":"aut"},{"display":"Haberkorn, Uwe","given":"Uwe","family":"Haberkorn","role":"aut"},{"family":"Weber","role":"aut","display":"Weber, Wolfgang A.","given":"Wolfgang A."}],"recId":"1687645442","note":["Gesehen am 17.01.2019","Im Titel ist \"68\" in 68Ga-labeled hochgestellt"],"name":{"displayForm":["Zohreh Varasteh, Sarajo Mohanta, Stephanie Robu, Miriam Braeuer, Yuanfang Li, Negar Omidvari, Geoffrey Topping, Ting Sun, Stephan G. Nekolla, Antonia Richter, Christian Weber, Andreas Habenicht, Uwe A. Haberkorn, and Wolfgang A. Weber"]},"language":["eng"],"relHost":[{"note":["Gesehen am 13.12.2021"],"pubHistory":["Nachgewiesen 5.1964 -"],"language":["eng"],"part":{"volume":"60","year":"2019","text":"60(2019), 12, Seite 1743-1749","pages":"1743-1749","extent":"7","issue":"12"},"titleAlt":[{"title":"JNM"}],"corporate":[{"display":"Society of Nuclear Medicine","role":"isb"}],"disp":"Molecular imaging of fibroblast activity after myocardial infarction using a 68Ga-labeled fibroblast activation protein inhibitor, FAPI-04Journal of nuclear medicine","type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"325793603","name":{"displayForm":["Society of Nuclear Medicine"]},"origin":[{"dateIssuedKey":"1964","publisherPlace":"New York, NY","publisher":"Soc.","dateIssuedDisp":"1964-"}],"title":[{"title_sort":"Journal of nuclear medicine","title":"Journal of nuclear medicine","subtitle":"JNM"}],"id":{"zdb":["2040222-3"],"eki":["325793603"],"issn":["2159-662X","1535-5667"]},"physDesc":[{"extent":"Online-Ressource"}]}],"origin":[{"dateIssuedKey":"2019","dateIssuedDisp":"August 12, 2019"}],"title":[{"title":"Molecular imaging of fibroblast activity after myocardial infarction using a 68Ga-labeled fibroblast activation protein inhibitor, FAPI-04","title_sort":"Molecular imaging of fibroblast activity after myocardial infarction using a 68Ga-labeled fibroblast activation protein inhibitor, FAPI-04"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"physDesc":[{"extent":"7 S."}],"id":{"doi":["10.2967/jnumed.119.226993"],"eki":["1687645442"]}} | ||
| SRT | |a VARASTEHZOMOLECULARI1220 | ||