A solid-phase transfection platform for arrayed CRISPR screens
Abstract Arrayed CRISPR-based screens emerge as a powerful alternative to pooled screens making it possible to investigate a wide range of cellular phenotypes that are typically not amenable to pooled screens. Here, we describe a solid-phase transfection platform that enables CRISPR-based genetic sc...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
23 December 2019
|
| In: |
Molecular systems biology
Year: 2019, Volume: 15, Issue: 12 |
| ISSN: | 1744-4292 |
| DOI: | 10.15252/msb.20198983 |
| Online Access: | Verlag, Volltext: https://doi.org/10.15252/msb.20198983 Verlag: https://www.embopress.org/doi/full/10.15252/msb.20198983 |
| Author Notes: | Özdemirhan Serçin, Sabine Reither, Paris Roidos, Nadja Ballin, Spyridon Palikyras, Anna Baginska, Katrin Rein, Maria Llamazares, Aliaksandr Halavatyi, Hauke Winter, Thomas Muley, Renata Z. Jurkowska, Amir Abdollahi, Frank T. Zenke, Beate Neumann & Balca R. Mardin |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1688467874 | ||
| 003 | DE-627 | ||
| 005 | 20220817213128.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200124s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.15252/msb.20198983 |2 doi | |
| 035 | |a (DE-627)1688467874 | ||
| 035 | |a (DE-599)KXP1688467874 | ||
| 035 | |a (OCoLC)1341299199 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Serçin, Özdemirhan |e VerfasserIn |0 (DE-588)1203389361 |0 (DE-627)1688468714 |4 aut | |
| 245 | 1 | 2 | |a A solid-phase transfection platform for arrayed CRISPR screens |c Özdemirhan Serçin, Sabine Reither, Paris Roidos, Nadja Ballin, Spyridon Palikyras, Anna Baginska, Katrin Rein, Maria Llamazares, Aliaksandr Halavatyi, Hauke Winter, Thomas Muley, Renata Z. Jurkowska, Amir Abdollahi, Frank T. Zenke, Beate Neumann & Balca R. Mardin |
| 264 | 1 | |c 23 December 2019 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 24.01.2020 | ||
| 520 | |a Abstract Arrayed CRISPR-based screens emerge as a powerful alternative to pooled screens making it possible to investigate a wide range of cellular phenotypes that are typically not amenable to pooled screens. Here, we describe a solid-phase transfection platform that enables CRISPR-based genetic screens in arrayed format with flexible readouts. We demonstrate efficient gene knockout upon delivery of guide RNAs and Cas9/guide RNA ribonucleoprotein complexes into untransformed and cancer cell lines. In addition, we provide evidence that our platform can be easily adapted to high-throughput screens and we use this approach to study oncogene addiction in tumor cells. Finally demonstrating that the human primary cells can also be edited using this method, we pave the way for rapid testing of potential targeted therapies. | ||
| 650 | 4 | |a arrayed screens | |
| 650 | 4 | |a CRISPR/Cas9 | |
| 650 | 4 | |a gRNA/RNP delivery | |
| 650 | 4 | |a solid phase | |
| 650 | 4 | |a transfection platform | |
| 700 | 1 | |a Rein, Katrin |e VerfasserIn |0 (DE-588)1203389639 |0 (DE-627)1688469338 |4 aut | |
| 700 | 1 | |a Halavatyi, Aliaksandr |e VerfasserIn |0 (DE-588)1203389957 |0 (DE-627)168846980X |4 aut | |
| 700 | 1 | |a Winter, Hauke |e VerfasserIn |0 (DE-588)1162076038 |0 (DE-627)1025509773 |0 (DE-576)176791531 |4 aut | |
| 700 | 1 | |a Abdollahi, Amir |e VerfasserIn |0 (DE-588)129612715 |0 (DE-627)474757765 |0 (DE-576)297748874 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular systems biology |d [London] : Nature Publishing Group UK, 2005 |g 15(2019,12) Artikel-Nummer e8983, 13 Seiten |h Online-Ressource |w (DE-627)490536905 |w (DE-600)2193510-5 |w (DE-576)28127942X |x 1744-4292 |7 nnas |a A solid-phase transfection platform for arrayed CRISPR screens |
| 773 | 1 | 8 | |g volume:15 |g year:2019 |g number:12 |g extent:13 |a A solid-phase transfection platform for arrayed CRISPR screens |
| 856 | 4 | 0 | |u https://doi.org/10.15252/msb.20198983 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://www.embopress.org/doi/full/10.15252/msb.20198983 |x Verlag |
| 951 | |a AR | ||
| 992 | |a 20200124 | ||
| 993 | |a Article | ||
| 994 | |a 2019 | ||
| 998 | |g 129612715 |a Abdollahi, Amir |m 129612715:Abdollahi, Amir |d 910000 |d 911400 |e 910000PA129612715 |e 911400PA129612715 |k 0/910000/ |k 1/910000/911400/ |p 13 | ||
| 998 | |g 1162076038 |a Winter, Hauke |m 1162076038:Winter, Hauke |d 50000 |e 50000PW1162076038 |k 0/50000/ |p 10 | ||
| 998 | |g 1203389957 |a Halavatyi, Aliaksandr |m 1203389957:Halavatyi, Aliaksandr |d 50000 |e 50000PH1203389957 |k 0/50000/ |p 9 | ||
| 998 | |g 1203389639 |a Rein, Katrin |m 1203389639:Rein, Katrin |d 910000 |d 911400 |e 910000PR1203389639 |e 911400PR1203389639 |k 0/910000/ |k 1/910000/911400/ |p 7 | ||
| 999 | |a KXP-PPN1688467874 |e 3581395061 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedKey":"2019","dateIssuedDisp":"23 December 2019"}],"id":{"doi":["10.15252/msb.20198983"],"eki":["1688467874"]},"recId":"1688467874","relHost":[{"id":{"doi":["10.1002/(ISSN)1744-4292"],"issn":["1744-4292"],"eki":["490536905"],"zdb":["2193510-5"]},"origin":[{"publisher":"Nature Publishing Group UK ; EMBO Press ; Wiley ; Nature Publ. Group [u.a.]","dateIssuedDisp":"[2005]-","publisherPlace":"[London] ; Heidelberg ; Hoboken, NJ [u.a.] ; London [u.a.]"}],"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"title":[{"title":"Molecular systems biology","title_sort":"Molecular systems biology"}],"note":["Gesehen am 09.12.2025"],"recId":"490536905","type":{"media":"Online-Ressource","bibl":"periodical"},"pubHistory":["1.2005 -"],"disp":"A solid-phase transfection platform for arrayed CRISPR screensMolecular systems biology","part":{"text":"15(2019,12) Artikel-Nummer e8983, 13 Seiten","year":"2019","extent":"13","issue":"12","volume":"15"}}],"person":[{"given":"Özdemirhan","role":"aut","family":"Serçin","display":"Serçin, Özdemirhan"},{"role":"aut","display":"Rein, Katrin","family":"Rein","given":"Katrin"},{"given":"Aliaksandr","display":"Halavatyi, Aliaksandr","family":"Halavatyi","role":"aut"},{"display":"Winter, Hauke","family":"Winter","role":"aut","given":"Hauke"},{"given":"Amir","role":"aut","display":"Abdollahi, Amir","family":"Abdollahi"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 24.01.2020"],"name":{"displayForm":["Özdemirhan Serçin, Sabine Reither, Paris Roidos, Nadja Ballin, Spyridon Palikyras, Anna Baginska, Katrin Rein, Maria Llamazares, Aliaksandr Halavatyi, Hauke Winter, Thomas Muley, Renata Z. Jurkowska, Amir Abdollahi, Frank T. Zenke, Beate Neumann & Balca R. Mardin"]},"title":[{"title_sort":"solid-phase transfection platform for arrayed CRISPR screens","title":"A solid-phase transfection platform for arrayed CRISPR screens"}],"physDesc":[{"extent":"13 S."}],"language":["eng"]} | ||
| SRT | |a SERCINOEZDSOLIDPHASE2320 | ||