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Molecular insight into RNA polymerase I promoter recognition and promoter melting

RNA polymerase I (Pol I) assembles with core factor (CF) and Rrn3 on the rDNA core promoter for transcription initiation. Here, we report cryo-EM structures of closed, intermediate and open Pol I initiation complexes from 2.7 to 3.7 Å resolution to visualize Pol I promoter melting and to structural...

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Bibliographic Details
Main Authors: Sadian, Yashar (Author) , Baudin, Florence (Author) , Tafur, Lucas (Author) , Murciano, Brice (Author) , Wetzel, Rene (Author) , Weis, Felix (Author) , Müller, Christoph W. (Author)
Format: Article (Journal)
Language:English
Published: 5 Dec 2019
In: Nature Communications
Year: 2019, Volume: 10
ISSN:2041-1723
DOI:10.1038/s41467-019-13510-w
Online Access:Verlag, Volltext: https://doi.org/10.1038/s41467-019-13510-w
Verlag, Volltext: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895186/
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Author Notes:Yashar Sadian, Florence Baudin, Lucas Tafur, Brice Murciano, Rene Wetzel, Felix Weis, Christoph W. Müller
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Summary:RNA polymerase I (Pol I) assembles with core factor (CF) and Rrn3 on the rDNA core promoter for transcription initiation. Here, we report cryo-EM structures of closed, intermediate and open Pol I initiation complexes from 2.7 to 3.7 Å resolution to visualize Pol I promoter melting and to structurally and biochemically characterize the recognition mechanism of Pol I promoter DNA. In the closed complex, double-stranded DNA runs outside the DNA-binding cleft. Rotation of CF and upstream DNA with respect to Pol I and Rrn3 results in the spontaneous loading and opening of the promoter followed by cleft closure and positioning of the Pol I A49 tandem winged helix domain (tWH) onto DNA. Conformational rearrangement of A49 tWH leads to a clash with Rrn3 to initiate complex disassembly and promoter escape. Comprehensive insight into the Pol I transcription initiation cycle allows comparisons with promoter opening by Pol II and Pol III., RNA polymerase I (Pol I) catalyses the transcription of pre-ribosomal RNA and for transcription initiation Pol I assembles with core factor and Rrn3 on the rDNA core promoter. Here the authors provide insights into the molecular mechanism of promoter opening by Pol I by determining the cryo-EM structures of two closed, two intermediate and two open Pol I pre-initiation complexes.
Item Description:Gesehen am 19.02.2020
Physical Description:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-019-13510-w

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