Low cell-matrix adhesion reveals two subtypes of human pluripotent stem cells
We show that a human pluripotent stem cell (hPSC) population cultured on a low-adhesion substrate developed two hPSC subtypes with different colony morphologies: flat and domed. Notably, the dome-like cells showed higher active proliferation capacity and increased several pluripotent genes’ expressi...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
June 28, 2018
|
| In: |
Stem cell reports
Year: 2018, Jahrgang: 11, Heft: 1, Pages: 142-156 |
| ISSN: | 2213-6711 |
| DOI: | 10.1016/j.stemcr.2018.06.003 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.stemcr.2018.06.003 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S2213671118302662 
|
| Verfasserangaben: | Leqian Yu, Junjun Li, Jiayin Hong, Yasuhiro Takashima, Nanae Fujimoto, Minako Nakajima, Akihisa Yamamoto, Xiaofeng Dong, Yujiao Dang, Yu Hou, Wei Yang, Itsunari Minami, Keisuke Okita, Motomu Tanaka, Chunxiong Luo, Fuchou Tang, Yong Chen, Chao Tang, Hidetoshi Kotera, and Li Liu |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1691796778 | ||
| 003 | DE-627 | ||
| 005 | 20230426073019.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200305s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.stemcr.2018.06.003 |2 doi | |
| 035 | |a (DE-627)1691796778 | ||
| 035 | |a (DE-599)KXP1691796778 | ||
| 035 | |a (OCoLC)1341309571 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 30 |2 sdnb | ||
| 100 | 1 | |a Yu, Leqian |e VerfasserIn |0 (DE-588)1205950338 |0 (DE-627)1691796581 |4 aut | |
| 245 | 1 | 0 | |a Low cell-matrix adhesion reveals two subtypes of human pluripotent stem cells |c Leqian Yu, Junjun Li, Jiayin Hong, Yasuhiro Takashima, Nanae Fujimoto, Minako Nakajima, Akihisa Yamamoto, Xiaofeng Dong, Yujiao Dang, Yu Hou, Wei Yang, Itsunari Minami, Keisuke Okita, Motomu Tanaka, Chunxiong Luo, Fuchou Tang, Yong Chen, Chao Tang, Hidetoshi Kotera, and Li Liu |
| 264 | 1 | |c June 28, 2018 | |
| 300 | |a 15 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 05.03.2020 | ||
| 520 | |a We show that a human pluripotent stem cell (hPSC) population cultured on a low-adhesion substrate developed two hPSC subtypes with different colony morphologies: flat and domed. Notably, the dome-like cells showed higher active proliferation capacity and increased several pluripotent genes’ expression compared with the flat monolayer cells. We further demonstrated that cell-matrix adhesion mediates the interaction between cell morphology and expression of KLF4 and KLF5 through a serum response factor (SRF)-based regulatory double loop. Our results provide a mechanistic view on the coupling among adhesion, stem cell morphology, and pluripotency, shedding light on the critical role of cell-matrix adhesion in the induction and maintenance of hPSC. | ||
| 650 | 4 | |a cell-matrix adhesion | |
| 650 | 4 | |a heterogeneity | |
| 650 | 4 | |a human pluripotent stem cells | |
| 650 | 4 | |a mathematical model | |
| 650 | 4 | |a nanofiber | |
| 650 | 4 | |a serum response factor | |
| 650 | 4 | |a single-cell culture | |
| 650 | 4 | |a stem cell morphology | |
| 700 | 1 | |a Li, Junjun |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hong, Jiayin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Takashima, Yasuhiro |e VerfasserIn |4 aut | |
| 700 | 1 | |a Fujimoto, Nanae |e VerfasserIn |4 aut | |
| 700 | 1 | |a Nakajima, Minako |e VerfasserIn |4 aut | |
| 700 | 1 | |a Yamamoto, Akihisa |e VerfasserIn |0 (DE-588)1156564840 |0 (DE-627)1019363118 |0 (DE-576)502257032 |4 aut | |
| 700 | 1 | |a Dong, Xiaofeng |e VerfasserIn |4 aut | |
| 700 | 1 | |a Dang, Yujiao |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hou, Yu |e VerfasserIn |4 aut | |
| 700 | 1 | |a Yang, Wei |e VerfasserIn |4 aut | |
| 700 | 1 | |a Minami, Itsunari |e VerfasserIn |4 aut | |
| 700 | 1 | |a Okita, Keisuke |e VerfasserIn |4 aut | |
| 700 | 1 | |a Tanaka, Motomu |e VerfasserIn |0 (DE-588)1060648377 |0 (DE-627)800414829 |0 (DE-576)416774636 |4 aut | |
| 700 | 1 | |a Luo, Chunxiong |e VerfasserIn |4 aut | |
| 700 | 1 | |a Tang, Fuchou |e VerfasserIn |4 aut | |
| 700 | 1 | |a Chen, Yong |e VerfasserIn |4 aut | |
| 700 | 1 | |a Tang, Chao |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kotera, Hidetoshi |e VerfasserIn |4 aut | |
| 700 | 1 | |a Liu, Li |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Stem cell reports |d Maryland Heights, MO : Cell Press, 2013 |g 11(2018), 1, Seite 142-156 |h Online-Ressource |w (DE-627)749730862 |w (DE-600)2720528-9 |w (DE-576)383773474 |x 2213-6711 |7 nnas |a Low cell-matrix adhesion reveals two subtypes of human pluripotent stem cells |
| 773 | 1 | 8 | |g volume:11 |g year:2018 |g number:1 |g pages:142-156 |g extent:15 |a Low cell-matrix adhesion reveals two subtypes of human pluripotent stem cells |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.stemcr.2018.06.003 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S2213671118302662 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200305 | ||
| 993 | |a Article | ||
| 994 | |a 2018 | ||
| 998 | |g 1060648377 |a Tanaka, Motomu |m 1060648377:Tanaka, Motomu |d 120000 |d 120300 |e 120000PT1060648377 |e 120300PT1060648377 |k 0/120000/ |k 1/120000/120300/ |p 14 | ||
| 999 | |a KXP-PPN1691796778 |e 3604185963 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"title":[{"title":"Low cell-matrix adhesion reveals two subtypes of human pluripotent stem cells","title_sort":"Low cell-matrix adhesion reveals two subtypes of human pluripotent stem cells"}],"note":["Gesehen am 05.03.2020"],"origin":[{"dateIssuedDisp":"June 28, 2018","dateIssuedKey":"2018"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"person":[{"family":"Yu","display":"Yu, Leqian","role":"aut","given":"Leqian"},{"given":"Junjun","role":"aut","family":"Li","display":"Li, Junjun"},{"role":"aut","given":"Jiayin","display":"Hong, Jiayin","family":"Hong"},{"display":"Takashima, Yasuhiro","family":"Takashima","role":"aut","given":"Yasuhiro"},{"family":"Fujimoto","display":"Fujimoto, Nanae","given":"Nanae","role":"aut"},{"given":"Minako","role":"aut","display":"Nakajima, Minako","family":"Nakajima"},{"display":"Yamamoto, Akihisa","family":"Yamamoto","role":"aut","given":"Akihisa"},{"given":"Xiaofeng","role":"aut","family":"Dong","display":"Dong, Xiaofeng"},{"display":"Dang, Yujiao","family":"Dang","role":"aut","given":"Yujiao"},{"family":"Hou","display":"Hou, Yu","role":"aut","given":"Yu"},{"display":"Yang, Wei","family":"Yang","role":"aut","given":"Wei"},{"family":"Minami","display":"Minami, Itsunari","role":"aut","given":"Itsunari"},{"family":"Okita","display":"Okita, Keisuke","given":"Keisuke","role":"aut"},{"given":"Motomu","role":"aut","display":"Tanaka, Motomu","family":"Tanaka"},{"family":"Luo","display":"Luo, Chunxiong","role":"aut","given":"Chunxiong"},{"display":"Tang, Fuchou","family":"Tang","given":"Fuchou","role":"aut"},{"role":"aut","given":"Yong","family":"Chen","display":"Chen, Yong"},{"display":"Tang, Chao","family":"Tang","given":"Chao","role":"aut"},{"display":"Kotera, Hidetoshi","family":"Kotera","given":"Hidetoshi","role":"aut"},{"role":"aut","given":"Li","display":"Liu, Li","family":"Liu"}],"relHost":[{"language":["eng"],"note":["Gesehen am 25.08.20"],"origin":[{"dateIssuedKey":"2013","publisher":"Cell Press ; Elsevier","dateIssuedDisp":"2013-","publisherPlace":"Maryland Heights, MO ; [New York, NY]"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"title":[{"title_sort":"Stem cell reports","title":"Stem cell reports"}],"part":{"issue":"1","year":"2018","pages":"142-156","text":"11(2018), 1, Seite 142-156","extent":"15","volume":"11"},"disp":"Low cell-matrix adhesion reveals two subtypes of human pluripotent stem cellsStem cell reports","id":{"eki":["749730862"],"issn":["2213-6711"],"zdb":["2720528-9"]},"pubHistory":["1.2013 -"],"recId":"749730862","name":{"displayForm":["International Society for Stem Cell Research"]},"physDesc":[{"extent":"Online-Ressource"}]}],"id":{"eki":["1691796778"],"doi":["10.1016/j.stemcr.2018.06.003"]},"recId":"1691796778","name":{"displayForm":["Leqian Yu, Junjun Li, Jiayin Hong, Yasuhiro Takashima, Nanae Fujimoto, Minako Nakajima, Akihisa Yamamoto, Xiaofeng Dong, Yujiao Dang, Yu Hou, Wei Yang, Itsunari Minami, Keisuke Okita, Motomu Tanaka, Chunxiong Luo, Fuchou Tang, Yong Chen, Chao Tang, Hidetoshi Kotera, and Li Liu"]},"physDesc":[{"extent":"15 S."}]} | ||
| SRT | |a YULEQIANLILOWCELLMAT2820 | ||