Buchumschlag

Low cell-matrix adhesion reveals two subtypes of human pluripotent stem cells

We show that a human pluripotent stem cell (hPSC) population cultured on a low-adhesion substrate developed two hPSC subtypes with different colony morphologies: flat and domed. Notably, the dome-like cells showed higher active proliferation capacity and increased several pluripotent genes’ expressi...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Yu, Leqian (VerfasserIn) , Li, Junjun (VerfasserIn) , Hong, Jiayin (VerfasserIn) , Takashima, Yasuhiro (VerfasserIn) , Fujimoto, Nanae (VerfasserIn) , Nakajima, Minako (VerfasserIn) , Yamamoto, Akihisa (VerfasserIn) , Dong, Xiaofeng (VerfasserIn) , Dang, Yujiao (VerfasserIn) , Hou, Yu (VerfasserIn) , Yang, Wei (VerfasserIn) , Minami, Itsunari (VerfasserIn) , Okita, Keisuke (VerfasserIn) , Tanaka, Motomu (VerfasserIn) , Luo, Chunxiong (VerfasserIn) , Tang, Fuchou (VerfasserIn) , Chen, Yong (VerfasserIn) , Tang, Chao (VerfasserIn) , Kotera, Hidetoshi (VerfasserIn) , Liu, Li (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: June 28, 2018
In: Stem cell reports
Year: 2018, Jahrgang: 11, Heft: 1, Pages: 142-156
ISSN:2213-6711
DOI:10.1016/j.stemcr.2018.06.003
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.stemcr.2018.06.003
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S2213671118302662
Volltext
Verfasserangaben:Leqian Yu, Junjun Li, Jiayin Hong, Yasuhiro Takashima, Nanae Fujimoto, Minako Nakajima, Akihisa Yamamoto, Xiaofeng Dong, Yujiao Dang, Yu Hou, Wei Yang, Itsunari Minami, Keisuke Okita, Motomu Tanaka, Chunxiong Luo, Fuchou Tang, Yong Chen, Chao Tang, Hidetoshi Kotera, and Li Liu
Beschreibung
Zusammenfassung:We show that a human pluripotent stem cell (hPSC) population cultured on a low-adhesion substrate developed two hPSC subtypes with different colony morphologies: flat and domed. Notably, the dome-like cells showed higher active proliferation capacity and increased several pluripotent genes’ expression compared with the flat monolayer cells. We further demonstrated that cell-matrix adhesion mediates the interaction between cell morphology and expression of KLF4 and KLF5 through a serum response factor (SRF)-based regulatory double loop. Our results provide a mechanistic view on the coupling among adhesion, stem cell morphology, and pluripotency, shedding light on the critical role of cell-matrix adhesion in the induction and maintenance of hPSC.
Beschreibung:Gesehen am 05.03.2020
Beschreibung:Online Resource
ISSN:2213-6711
DOI:10.1016/j.stemcr.2018.06.003

Ähnliche Einträge