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Optimized base editors enable efficient editing in cells, organoids and mice

CRISPR base editing enables the creation of targeted single-base conversions without generating double-stranded breaks. However, the efficiency of current base editors is very low in many cell types. We reengineered the sequences of BE3, BE4Gam, and xBE3 by codon optimization and incorporation of ad...

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Hauptverfasser: Zafra, Maria Paz (VerfasserIn) , Schatoff, Emma M. (VerfasserIn) , Katti, Alyna (VerfasserIn) , Foronda, Miguel (VerfasserIn) , Breinig, Marco (VerfasserIn) , Schweitzer, Anabel (VerfasserIn) , Simon, Amber (VerfasserIn) , Han, Teng (VerfasserIn) , Goswami, Sukanya (VerfasserIn) , Montgomery, Emma (VerfasserIn) , Thibado, Jordana (VerfasserIn) , Kastenhuber, Edward R. (VerfasserIn) , Sánchez-Rivera, Francisco J. (VerfasserIn) , Shi, Junwei (VerfasserIn) , Vakoc, Christopher R. (VerfasserIn) , Lowe, Scott W. (VerfasserIn) , Tschaharganeh, Darjus-Felix (VerfasserIn) , Dow, Lukas E. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 3 July 2018
In: Nature biotechnology
Year: 2018, Jahrgang: 36, Heft: 9, Pages: 888-893
ISSN:1546-1696
DOI:10.1038/nbt.4194
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/nbt.4194
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Verfasserangaben:Maria Paz Zafra, Emma M. Schatoff, Alyna Katti, Miguel Foronda, Marco Breinig, Anabel Y. Schweitzer, Amber Simon, Teng Han, Sukanya Goswami, Emma Montgomery, Jordana Thibado, Edward R. Kastenhuber, Francisco J. Sánchez-Rivera, Junwei Shi, Christopher R. Vakoc, Scott W. Lowe, Darjus F. Tschaharganeh & Lukas E. Dow

MARC

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