Improving electrical properties of iPSC-cardiomyocytes by enhancing Cx43 expression
Abstract: The therapeutic potential of induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is limited by immature functional features including low impulse propagation and reduced cell excitability. Key players regulating electrical activity are voltage-gated Na<sup>+</sup> c...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
16 May 2018
|
| In: |
Journal of molecular and cellular cardiology
Year: 2018, Jahrgang: 120, Pages: 31-41 |
| ISSN: | 1095-8584 |
| DOI: | 10.1016/j.yjmcc.2018.05.010 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.yjmcc.2018.05.010 Verlag, lizenzpflichtig, Volltext: https://www.jmmc-online.com/article/S0022-2828(18)30171-8/abstract 
|
| Verfasserangaben: | Valentin Sottas, Carl-Mattheis Wahl, Mihnea C. Trache, Michael Bartolf-Kopp, Sidney Cambridge, Markus Hecker, Nina D. Ullrich |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1693473941 | ||
| 003 | DE-627 | ||
| 005 | 20220818022353.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200330s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.yjmcc.2018.05.010 |2 doi | |
| 035 | |a (DE-627)1693473941 | ||
| 035 | |a (DE-599)KXP1693473941 | ||
| 035 | |a (OCoLC)1341311483 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Sottas, Valentin |d 1984- |e VerfasserIn |0 (DE-588)1084019841 |0 (DE-627)848090071 |0 (DE-576)456143211 |4 aut | |
| 245 | 1 | 0 | |a Improving electrical properties of iPSC-cardiomyocytes by enhancing Cx43 expression |c Valentin Sottas, Carl-Mattheis Wahl, Mihnea C. Trache, Michael Bartolf-Kopp, Sidney Cambridge, Markus Hecker, Nina D. Ullrich |
| 264 | 1 | |c 16 May 2018 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 30.03.2020 | ||
| 520 | |a Abstract: The therapeutic potential of induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is limited by immature functional features including low impulse propagation and reduced cell excitability. Key players regulating electrical activity are voltage-gated Na<sup>+</sup> channels (Na<sub>v</sub>1.5) and gap junctions built from connexin-43 (Cx43). Here we tested the hypothesis that enhanced Cx43 expression increases intercellular coupling and influences excitability by modulating Na<sub>v</sub>1.5. Using transgenic approaches, Cx43 and Na<sub>v</sub>1.5 localization and cell coupling were studied by confocal imaging. Na<sub>v</sub>1.5 currents and action potentials (APs) were measured using the patch-clamp technique. Enhanced sarcolemmal Cx43 expression significantly improved intercellular coupling and accelerated dye transfer kinetics. Furthermore, Cx43 modulated Na<sub>v</sub>1.5 function leading to significantly higher current and enhanced AP upstroke velocities, thereby improving electrical activity as measured by microelectrode arrays. These findings suggest a mechanistic link between cell coupling and excitability controlled by Cx43 expression in iPSC-CMs. Therefore, we propose Cx43 as novel molecular target for improving electrical properties of iPSC-CMs to match the functional properties of native myocytes. | ||
| 700 | 1 | |a Wahl, Carl-Mattheis |d 1995- |e VerfasserIn |0 (DE-588)1207338702 |0 (DE-627)1693606879 |4 aut | |
| 700 | 1 | |a Trache, Cristian |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bartolf-Kopp, Michael |e VerfasserIn |0 (DE-588)1207338990 |0 (DE-627)1693607573 |4 aut | |
| 700 | 1 | |a Cambridge, Sidney |e VerfasserIn |0 (DE-588)1054299390 |0 (DE-627)791264823 |0 (DE-576)410107409 |4 aut | |
| 700 | 1 | |a Hecker, Markus |d 1960- |e VerfasserIn |0 (DE-588)1071693344 |0 (DE-627)826266037 |0 (DE-576)433260319 |4 aut | |
| 700 | 1 | |a Ullrich, Nina D. |e VerfasserIn |0 (DE-588)1130195619 |0 (DE-627)884562549 |0 (DE-576)486667251 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of molecular and cellular cardiology |d New York, NY [u.a.] : Elsevier, 1970 |g 120(2018), Seite 31-41 |h Online-Ressource |w (DE-627)267328095 |w (DE-600)1469767-1 |w (DE-576)104193913 |x 1095-8584 |7 nnas |a Improving electrical properties of iPSC-cardiomyocytes by enhancing Cx43 expression |
| 773 | 1 | 8 | |g volume:120 |g year:2018 |g pages:31-41 |g extent:11 |a Improving electrical properties of iPSC-cardiomyocytes by enhancing Cx43 expression |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.yjmcc.2018.05.010 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.jmmc-online.com/article/S0022-2828(18)30171-8/abstract |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200330 | ||
| 993 | |a Article | ||
| 994 | |a 2018 | ||
| 998 | |g 1130195619 |a Ullrich, Nina D. |m 1130195619:Ullrich, Nina D. |d 50000 |d 53200 |e 50000PU1130195619 |e 53200PU1130195619 |k 0/50000/ |k 1/50000/53200/ |p 7 |y j | ||
| 998 | |g 1071693344 |a Hecker, Markus |m 1071693344:Hecker, Markus |d 50000 |d 53200 |e 50000PH1071693344 |e 53200PH1071693344 |k 0/50000/ |k 1/50000/53200/ |p 6 | ||
| 998 | |g 1054299390 |a Cambridge, Sidney |m 1054299390:Cambridge, Sidney |d 50000 |d 53100 |e 50000PC1054299390 |e 53100PC1054299390 |k 0/50000/ |k 1/50000/53100/ |p 5 | ||
| 998 | |g 1207338990 |a Bartolf-Kopp, Michael |m 1207338990:Bartolf-Kopp, Michael |d 50000 |d 53100 |e 50000PB1207338990 |e 53100PB1207338990 |k 0/50000/ |k 1/50000/53100/ |p 4 | ||
| 998 | |g 1207338702 |a Wahl, Carl-Mattheis |m 1207338702:Wahl, Carl-Mattheis |d 50000 |d 53100 |e 50000PW1207338702 |e 53100PW1207338702 |k 0/50000/ |k 1/50000/53100/ |p 2 | ||
| 998 | |g 1084019841 |a Sottas, Valentin |m 1084019841:Sottas, Valentin |p 1 |x j | ||
| 999 | |a KXP-PPN1693473941 |e 3615655664 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 30.03.2020"],"origin":[{"dateIssuedDisp":"16 May 2018","dateIssuedKey":"2018"}],"relHost":[{"name":{"displayForm":["International Society for Heart Research"]},"recId":"267328095","id":{"eki":["267328095"],"issn":["1095-8584"],"zdb":["1469767-1"]},"pubHistory":["1.1970 -"],"origin":[{"publisherPlace":"New York, NY [u.a.] ; London","dateIssuedKey":"1970","publisher":"Elsevier ; Academic Press","dateIssuedDisp":"1970-"}],"note":["Gesehen am 21.01.20","Ungezählte Beil.: Supplement"],"physDesc":[{"extent":"Online-Ressource"}],"disp":"Improving electrical properties of iPSC-cardiomyocytes by enhancing Cx43 expressionJournal of molecular and cellular cardiology","type":{"bibl":"periodical","media":"Online-Ressource"},"language":["eng"],"corporate":[{"display":"International Society for Heart Research","role":"isb"}],"part":{"pages":"31-41","text":"120(2018), Seite 31-41","extent":"11","volume":"120","year":"2018"},"title":[{"title_sort":"Journal of molecular and cellular cardiology","title":"Journal of molecular and cellular cardiology"}]}],"person":[{"display":"Sottas, Valentin","role":"aut","family":"Sottas","given":"Valentin"},{"given":"Carl-Mattheis","family":"Wahl","role":"aut","display":"Wahl, Carl-Mattheis"},{"family":"Trache","given":"Cristian","role":"aut","display":"Trache, Cristian"},{"family":"Bartolf-Kopp","given":"Michael","display":"Bartolf-Kopp, Michael","role":"aut"},{"family":"Cambridge","given":"Sidney","role":"aut","display":"Cambridge, Sidney"},{"display":"Hecker, Markus","role":"aut","given":"Markus","family":"Hecker"},{"family":"Ullrich","given":"Nina D.","display":"Ullrich, Nina D.","role":"aut"}],"title":[{"title":"Improving electrical properties of iPSC-cardiomyocytes by enhancing Cx43 expression","title_sort":"Improving electrical properties of iPSC-cardiomyocytes by enhancing Cx43 expression"}],"physDesc":[{"extent":"11 S."}],"recId":"1693473941","name":{"displayForm":["Valentin Sottas, Carl-Mattheis Wahl, Mihnea C. Trache, Michael Bartolf-Kopp, Sidney Cambridge, Markus Hecker, Nina D. Ullrich"]},"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"doi":["10.1016/j.yjmcc.2018.05.010"],"eki":["1693473941"]}} | ||
| SRT | |a SOTTASVALEIMPROVINGE1620 | ||