Buchumschlag

Mechanism of transcription termination: PTRF interacts with the largest subunit of RNA polymerase I and dissociates paused transcription complexes from yeast and mouse

Transcription termination by RNA polymerase I (Pol I) is a stepwise process. First the elongating RNA polymerase is forced to pause by DNA-bound transcription termination factor (TTF-I). Then the ternary transcription complex is dissociated by PTRF, a novel factor that promotes release of both nasce...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Jansa, Pavel (VerfasserIn) , Grummt, Ingrid (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: November 1999
In: Molecular genetics and genomics
Year: 1999, Jahrgang: 262, Heft: 3, Pages: 508-514
ISSN:1617-4623
DOI:10.1007/s004380051112
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s004380051112
Volltext
Verfasserangaben:P. Jansa, I. Grummt

MARC

LEADER 00000caa a2200000 c 4500
001 1693497700
003 DE-627
005 20220818022623.0
007 cr uuu---uuuuu
008 200330s1999 xx |||||o 00| ||eng c
024 7 |a 10.1007/s004380051112  |2 doi 
035 |a (DE-627)1693497700 
035 |a (DE-599)KXP1693497700 
035 |a (OCoLC)1341311410 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Jansa, Pavel  |e VerfasserIn  |0 (DE-588)1207294179  |0 (DE-627)1693497298  |4 aut 
245 1 0 |a Mechanism of transcription termination  |b PTRF interacts with the largest subunit of RNA polymerase I and dissociates paused transcription complexes from yeast and mouse  |c P. Jansa, I. Grummt 
264 1 |c November 1999 
300 |a 7 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 30.03.2020 
520 |a Transcription termination by RNA polymerase I (Pol I) is a stepwise process. First the elongating RNA polymerase is forced to pause by DNA-bound transcription termination factor (TTF-I). Then the ternary transcription complex is dissociated by PTRF, a novel factor that promotes release of both nascent transcripts and Pol I from the template. In this study we have investigated the ability of PTRF to liberate transcripts from ternary transcription complexes isolated from yeast and mouse. Using immobilized, tailed templates that contain terminator sequences from Saccharomyces cerevisiae and mouse, respectively, we demonstrate that PTRF promotes release of terminated transcripts, irrespective of whether mouse Pol I has interacted with the murine termination factor TTF-I or its yeast homolog Reb1p. In contrast, mouse Pol I paused by the lac repressor remains bound to the template both in the presence and absence of PTRF. We demonstrate that PTRF interacts with the largest subunit of murine Pol I, with TTF-I and Reb1p, but not the lac repressor. The results imply that Pol I transcription termination in yeast and mouse is mediated by conserved interactions between Pol I, Reb1p/TTF-I and PTRF. 
700 1 |a Grummt, Ingrid  |d 1943-  |e VerfasserIn  |0 (DE-588)143378724  |0 (DE-627)645139602  |0 (DE-576)33674109X  |4 aut 
773 0 8 |i Enthalten in  |t Molecular genetics and genomics  |d Berlin : Springer, 1908  |g 262(1999), 3, Seite 508-514  |h Online-Ressource  |w (DE-627)254630243  |w (DE-600)1462070-4  |w (DE-576)074529455  |x 1617-4623  |7 nnas  |a Mechanism of transcription termination PTRF interacts with the largest subunit of RNA polymerase I and dissociates paused transcription complexes from yeast and mouse 
773 1 8 |g volume:262  |g year:1999  |g number:3  |g pages:508-514  |g extent:7  |a Mechanism of transcription termination PTRF interacts with the largest subunit of RNA polymerase I and dissociates paused transcription complexes from yeast and mouse 
856 4 0 |u https://doi.org/10.1007/s004380051112  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20200330 
993 |a Article 
994 |a 1999 
998 |g 143378724  |a Grummt, Ingrid  |m 143378724:Grummt, Ingrid  |d 140000  |e 140000PG143378724  |k 0/140000/  |p 2  |y j 
999 |a KXP-PPN1693497700  |e 3615714857 
BIB |a Y 
SER |a journal 
JSO |a {"title":[{"title":"Mechanism of transcription termination","subtitle":"PTRF interacts with the largest subunit of RNA polymerase I and dissociates paused transcription complexes from yeast and mouse","title_sort":"Mechanism of transcription termination"}],"person":[{"family":"Jansa","given":"Pavel","roleDisplay":"VerfasserIn","display":"Jansa, Pavel","role":"aut"},{"family":"Grummt","given":"Ingrid","roleDisplay":"VerfasserIn","display":"Grummt, Ingrid","role":"aut"}],"recId":"1693497700","language":["eng"],"note":["Gesehen am 30.03.2020"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"eki":["1693497700"],"doi":["10.1007/s004380051112"]},"origin":[{"dateIssuedDisp":"November 1999","dateIssuedKey":"1999"}],"name":{"displayForm":["P. Jansa, I. Grummt"]},"relHost":[{"id":{"eki":["254630243"],"zdb":["1462070-4"],"issn":["1617-4623","1432-1874"]},"origin":[{"dateIssuedDisp":"1908-","publisher":"Springer","dateIssuedKey":"1908","publisherPlace":"Berlin ; Heidelberg ; New York, NY"}],"physDesc":[{"extent":"Online-Ressource"}],"title":[{"subtitle":"MGG","title":"Molecular genetics and genomics","title_sort":"Molecular genetics and genomics"}],"recId":"254630243","language":["eng"],"disp":"Mechanism of transcription termination PTRF interacts with the largest subunit of RNA polymerase I and dissociates paused transcription complexes from yeast and mouseMolecular genetics and genomics","note":["Gesehen am 10.10.24"],"type":{"media":"Online-Ressource","bibl":"periodical"},"part":{"extent":"7","volume":"262","text":"262(1999), 3, Seite 508-514","issue":"3","pages":"508-514","year":"1999"},"titleAlt":[{"title":"MGG"},{"title":"Zeitschrift für induktive Abstammungs- und Vererbungslehre"},{"title":"Zeitschrift für Vererbungslehre"},{"title":"Molecular and general genetics"}],"pubHistory":["1.1908 -"]}],"physDesc":[{"extent":"7 S."}]} 
SRT |a JANSAPAVELMECHANISMO1999 

Ähnliche Einträge