Proximity-driven site-specific and covalent labeling of proteins with a TexasRed fluorophore Reacting (ReacTR) Peptide Tag
It is of vital importance to visualize proteins in living cells noninvasively in order to elucidate their functions. Here, we describe a fast, efficient, and one-step covalent protein labeling method utilizing a small peptide tag called TR512, which was previously engineered to bind to TexasRed fluo...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Chapter/Article |
| Language: | English |
| Published: |
24 May 2019
|
| In: |
Proximity labeling
Year: 2019, Pages: 179-190 |
| DOI: | 10.1007/978-1-4939-9537-0_14 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/978-1-4939-9537-0_14 |
| Author Notes: | Murat Sunbul, Andres Jäschke |
MARC
| LEADER | 00000caa a22000002c 4500 | ||
|---|---|---|---|
| 001 | 1694660893 | ||
| 003 | DE-627 | ||
| 005 | 20230427070516.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200416s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/978-1-4939-9537-0_14 |2 doi | |
| 035 | |a (DE-627)1694660893 | ||
| 035 | |a (DE-599)KXP1694660893 | ||
| 035 | |a (OCoLC)1341315134 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 30 |2 sdnb | ||
| 100 | 1 | |a Sünbül, Murat |e VerfasserIn |0 (DE-588)118481189X |0 (DE-627)166370385X |4 aut | |
| 245 | 1 | 0 | |a Proximity-driven site-specific and covalent labeling of proteins with a TexasRed fluorophore Reacting (ReacTR) Peptide Tag |c Murat Sunbul, Andres Jäschke |
| 264 | 1 | |c 24 May 2019 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 16.04.2020 | ||
| 520 | |a It is of vital importance to visualize proteins in living cells noninvasively in order to elucidate their functions. Here, we describe a fast, efficient, and one-step covalent protein labeling method utilizing a small peptide tag called TR512, which was previously engineered to bind to TexasRed fluorophore by phage display. To covalently label proteins with TexasRed fluorophore, proteins of interest (POI) were fused to a reactive TR512 (ReacTR) tag carrying two cysteine residues. Upon addition of TexasRed fluorophore conjugated to N-α-chloroacetamide, a cysteine group of the ReacTR tag rapidly reacts with the electrophilic N-α-chloroacetamide gs uroup due to the proximity effect by forming a covalent bond between the fluorophore and ReacTR tag. Our approach uses a small peptide tag and a small-molecule fluorophore for labeling; thereby minimal perturbation on the function and dynamics of the POI is expected. | ||
| 650 | 4 | |a Fluorescence microscopy | |
| 650 | 4 | |a Fluorette | |
| 650 | 4 | |a Fluorophore | |
| 650 | 4 | |a Live-cell imaging | |
| 650 | 4 | |a Peptide tags | |
| 650 | 4 | |a Protein imaging | |
| 650 | 4 | |a Protein labeling | |
| 650 | 4 | |a TexasRed | |
| 650 | 4 | |a TR512 | |
| 700 | 1 | |a Jäschke, Andres |d 1962- |e VerfasserIn |0 (DE-588)1025112261 |0 (DE-627)751682756 |0 (DE-576)170797481 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Proximity labeling |d New York, NY : Humana, 2019 |g (2019), Seite 179-190 |h 1 Online-Ressource (X, 231 p. 74 illus., 46 illus. in color) |w (DE-627)166673196X |z 9781493995370 |7 nnam |a Proximity-driven site-specific and covalent labeling of proteins with a TexasRed fluorophore Reacting (ReacTR) Peptide Tag |
| 773 | 1 | 8 | |g year:2019 |g pages:179-190 |g extent:12 |a Proximity-driven site-specific and covalent labeling of proteins with a TexasRed fluorophore Reacting (ReacTR) Peptide Tag |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-4939-9537-0_14 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200416 | ||
| 993 | |a BookComponentPart | ||
| 994 | |a 2019 | ||
| 998 | |g 1025112261 |a Jäschke, Andres |m 1025112261:Jäschke, Andres |d 160000 |d 160100 |e 160000PJ1025112261 |e 160100PJ1025112261 |k 0/160000/ |k 1/160000/160100/ |p 2 |y j | ||
| 998 | |g 118481189X |a Sünbül, Murat |m 118481189X:Sünbül, Murat |d 160000 |d 160100 |e 160000PS118481189X |e 160100PS118481189X |k 0/160000/ |k 1/160000/160100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1694660893 |e 3624017306 | ||
| BIB | |a Y | ||
| JSO | |a {"name":{"displayForm":["Murat Sunbul, Andres Jäschke"]},"note":["Gesehen am 16.04.2020"],"language":["eng"],"recId":"1694660893","origin":[{"dateIssuedDisp":"24 May 2019","dateIssuedKey":"2019"}],"id":{"eki":["1694660893"],"doi":["10.1007/978-1-4939-9537-0_14"]},"relHost":[{"disp":"Proximity-driven site-specific and covalent labeling of proteins with a TexasRed fluorophore Reacting (ReacTR) Peptide TagProximity labeling","part":{"year":"2019","text":"(2019), Seite 179-190","pages":"179-190","extent":"12"},"title":[{"subtitle":"methods and protocols","title":"Proximity labeling","title_sort":"Proximity labeling"}],"physDesc":[{"extent":"1 Online-Ressource (X, 231 p. 74 illus., 46 illus. in color)"}],"relMultPart":[{"id":{"zdb":["2493551-7"],"eki":["599671882"],"issn":["1940-6029"]},"type":{"bibl":"serial","media":"Online-Ressource"},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Methods in molecular biology","title":"Methods in molecular biology"}],"part":{"number_sort":["2008"],"number":["2008"]},"disp":"Methods in Molecular Biology","pubHistory":["1.1984 -"],"recId":"599671882","language":["eng"],"note":["Gesehen am 05.11.12"],"dispAlt":"Methods in molecular biology","origin":[{"publisherPlace":"Totowa, NJ ; [Heidelberg]","dateIssuedDisp":"1984-","publisher":"Humana Press ; [Springer]","dateIssuedKey":"1984"}]}],"type":{"media":"Online-Ressource","bibl":"edited-book"},"id":{"eki":["166673196X"],"doi":["10.1007/978-1-4939-9537-0"],"isbn":["9781493995370"]},"person":[{"display":"Sünbül, Murat","family":"Sünbül","given":"Murat","role":"edt"},{"given":"Andres","role":"edt","display":"Jäschke, Andres","family":"Jäschke"}],"origin":[{"publisherPlace":"New York, NY","dateIssuedKey":"2019","publisher":"Humana","dateIssuedDisp":"2019"}],"language":["eng"],"recId":"166673196X","name":{"displayForm":["edited by Murat Sunbul, Andres Jäschke"]}}],"title":[{"title_sort":"Proximity-driven site-specific and covalent labeling of proteins with a TexasRed fluorophore Reacting (ReacTR) Peptide Tag","title":"Proximity-driven site-specific and covalent labeling of proteins with a TexasRed fluorophore Reacting (ReacTR) Peptide Tag"}],"physDesc":[{"extent":"12 S."}],"type":{"media":"Online-Ressource","bibl":"chapter"},"person":[{"given":"Murat","role":"aut","display":"Sünbül, Murat","family":"Sünbül"},{"display":"Jäschke, Andres","family":"Jäschke","given":"Andres","role":"aut"}]} | ||
| SRT | |a SUENBUELMUPROXIMITYD2420 | ||