The hydrolysis of 6-phosphogluconolactone in the second step of pentose phosphate pathway occurs via a two-water mechanism
Hydrolysis reaction marks the basis of life yet the mechanism of this crucial biochemical reaction is not completely understood. We recently reported the mechanisms of hydrolysis of nucleoside triphosphate and phosphate monoester. These two reactions hydrolyze P-O-P and P-O-C linkages, respectively....
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
12 June 2018
|
| In: |
Biophysical chemistry
Year: 2018, Volume: 240, Pages: 98-106 |
| ISSN: | 1873-4200 |
| DOI: | 10.1016/j.bpc.2018.06.002 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.bpc.2018.06.002 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0301462218301352 
|
| Author Notes: | Tabeer Fatima, Sadaf Rani, Stefan Fischer, Thomas Efferth, Farooq Ahmad Kiani |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1695797574 | ||
| 003 | DE-627 | ||
| 005 | 20230426080158.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200423s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.bpc.2018.06.002 |2 doi | |
| 035 | |a (DE-627)1695797574 | ||
| 035 | |a (DE-599)KXP1695797574 | ||
| 035 | |a (OCoLC)1341316397 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 29 |2 sdnb | ||
| 100 | 1 | |a Fatima, Tabeer |e VerfasserIn |0 (DE-588)1208867334 |0 (DE-627)1695805518 |4 aut | |
| 245 | 1 | 4 | |a The hydrolysis of 6-phosphogluconolactone in the second step of pentose phosphate pathway occurs via a two-water mechanism |c Tabeer Fatima, Sadaf Rani, Stefan Fischer, Thomas Efferth, Farooq Ahmad Kiani |
| 264 | 1 | |c 12 June 2018 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 23.04.2020 | ||
| 520 | |a Hydrolysis reaction marks the basis of life yet the mechanism of this crucial biochemical reaction is not completely understood. We recently reported the mechanisms of hydrolysis of nucleoside triphosphate and phosphate monoester. These two reactions hydrolyze P-O-P and P-O-C linkages, respectively. Here, we present the mechanism of hydrolysis of δ-6-phosphogluconolactone, which is an important precursor in the second step of the pentose phosphate pathway. Its hydrolysis requires the cleavage of C-O-C linkage and its mechanism is hitherto unknown. We report three mechanisms of hydrolysis of δ-6-phosphogluconolactone based on density functional computations. In the energetically most favorable mechanism, two water molecules participate in the hydrolysis reaction and the mechanism is sequential, i.e., activation of the attacking water molecule (OH bond breaking) precedes that of the cleavage of the CO bond of the C-O-C linkage. The rate-limiting energy barrier of this mechanism is comparable to the reported experimental free energy barrier. This mechanism has similarities with the mechanism of triphosphate hydrolysis and that of hydrolytic cleavage of DNA in EcoRV enzyme. This two-water sequential hydrolysis mechanism could be the unified mechanism required for the hydrolysis of other hydrolysable species in living cells. | ||
| 650 | 4 | |a 6-Phosphogluconolactonase enzyme | |
| 650 | 4 | |a Catalytic mechanism | |
| 650 | 4 | |a Computational biochemistry | |
| 650 | 4 | |a Hydrolysis reaction | |
| 650 | 4 | |a Pentose phosphate pathway | |
| 700 | 1 | |a Rani, Sadaf |e VerfasserIn |4 aut | |
| 700 | 1 | |a Fischer, Stefan |e VerfasserIn |0 (DE-588)1026232961 |0 (DE-627)726307378 |0 (DE-576)371534100 |4 aut | |
| 700 | 1 | |a Efferth, Thomas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kiani, Farooq Ahmad |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biophysical chemistry |d Amsterdam [u.a.] : Elsevier Science, 1973 |g 240(2018), Seite 98-106 |h Online-Ressource |w (DE-627)306321335 |w (DE-600)1496385-1 |w (DE-576)093980736 |x 1873-4200 |7 nnas |a The hydrolysis of 6-phosphogluconolactone in the second step of pentose phosphate pathway occurs via a two-water mechanism |
| 773 | 1 | 8 | |g volume:240 |g year:2018 |g pages:98-106 |g extent:9 |a The hydrolysis of 6-phosphogluconolactone in the second step of pentose phosphate pathway occurs via a two-water mechanism |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.bpc.2018.06.002 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0301462218301352 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200423 | ||
| 993 | |a Article | ||
| 994 | |a 2018 | ||
| 998 | |g 1026232961 |a Fischer, Stefan |m 1026232961:Fischer, Stefan |d 700000 |d 708000 |e 700000PF1026232961 |e 708000PF1026232961 |k 0/700000/ |k 1/700000/708000/ |p 3 | ||
| 999 | |a KXP-PPN1695797574 |e 363128036X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"extent":"9 S."}],"relHost":[{"title":[{"title_sort":"Biophysical chemistry","subtitle":"an international journal devoted to the physics and chemistry of biological phenomena","title":"Biophysical chemistry"}],"recId":"306321335","language":["eng"],"note":["Gesehen am 09.11.2020"],"disp":"The hydrolysis of 6-phosphogluconolactone in the second step of pentose phosphate pathway occurs via a two-water mechanismBiophysical chemistry","type":{"media":"Online-Ressource","bibl":"periodical"},"part":{"pages":"98-106","year":"2018","extent":"9","volume":"240","text":"240(2018), Seite 98-106"},"pubHistory":["1.1973/74 - 159.2011; Vol. 160.2012 -"],"id":{"eki":["306321335"],"zdb":["1496385-1"],"issn":["1873-4200"]},"origin":[{"dateIssuedKey":"1973","publisher":"Elsevier Science","dateIssuedDisp":"1973-","publisherPlace":"Amsterdam [u.a.]"}],"physDesc":[{"extent":"Online-Ressource"}]}],"origin":[{"dateIssuedDisp":"12 June 2018","dateIssuedKey":"2018"}],"id":{"eki":["1695797574"],"doi":["10.1016/j.bpc.2018.06.002"]},"name":{"displayForm":["Tabeer Fatima, Sadaf Rani, Stefan Fischer, Thomas Efferth, Farooq Ahmad Kiani"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 23.04.2020"],"language":["eng"],"recId":"1695797574","title":[{"title_sort":"hydrolysis of 6-phosphogluconolactone in the second step of pentose phosphate pathway occurs via a two-water mechanism","title":"The hydrolysis of 6-phosphogluconolactone in the second step of pentose phosphate pathway occurs via a two-water mechanism"}],"person":[{"role":"aut","display":"Fatima, Tabeer","roleDisplay":"VerfasserIn","given":"Tabeer","family":"Fatima"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Rani, Sadaf","given":"Sadaf","family":"Rani"},{"family":"Fischer","given":"Stefan","display":"Fischer, Stefan","roleDisplay":"VerfasserIn","role":"aut"},{"role":"aut","display":"Efferth, Thomas","roleDisplay":"VerfasserIn","given":"Thomas","family":"Efferth"},{"family":"Kiani","given":"Farooq Ahmad","roleDisplay":"VerfasserIn","display":"Kiani, Farooq Ahmad","role":"aut"}]} | ||
| SRT | |a FATIMATABEHYDROLYSIS1220 | ||