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Isolation of the protein and RNA content of active sites of transcription from mammalian cells

Transcription factories contain all three mammalian RNA polymerases, each actively transcribing a different subset of genes. This protocol describes how to isolate large factory fragments for the analysis of associated protein and RNA content.

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Bibliographic Details
Main Authors: Melnik, Svitlana (Author) , Caudron-Herger, Maïwen (Author) , Brant, Lilija (Author) , Carr, Ian M. (Author) , Rippe, Karsten (Author) , Cook, Peter R. (Author) , Papantonis, Argyris (Author)
Format: Article (Journal)
Language:English
Published: 25 February 2016
In: Nature protocols
Year: 2016, Volume: 11, Issue: 3, Pages: 553-565
ISSN:1750-2799
DOI:10.1038/nprot.2016.032
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/nprot.2016.032
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/nprot.2016.032
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Author Notes:Svitlana Melnik, Maïwen Caudron-Herger, Lilija Brant, Ian M. Carr, Karsten Rippe, Peter R Cook & Argyris Papantonis
Description
Summary:Transcription factories contain all three mammalian RNA polymerases, each actively transcribing a different subset of genes. This protocol describes how to isolate large factory fragments for the analysis of associated protein and RNA content.
Item Description:Gesehen am 05.05.2020
Physical Description:Online Resource
ISSN:1750-2799
DOI:10.1038/nprot.2016.032

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