Buchumschlag

Isolation of the protein and RNA content of active sites of transcription from mammalian cells

Transcription factories contain all three mammalian RNA polymerases, each actively transcribing a different subset of genes. This protocol describes how to isolate large factory fragments for the analysis of associated protein and RNA content.

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Melnik, Svitlana (VerfasserIn) , Caudron-Herger, Maïwen (VerfasserIn) , Brant, Lilija (VerfasserIn) , Carr, Ian M. (VerfasserIn) , Rippe, Karsten (VerfasserIn) , Cook, Peter R. (VerfasserIn) , Papantonis, Argyris (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 25 February 2016
In: Nature protocols
Year: 2016, Jahrgang: 11, Heft: 3, Pages: 553-565
ISSN:1750-2799
DOI:10.1038/nprot.2016.032
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/nprot.2016.032
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/nprot.2016.032
Volltext
Verfasserangaben:Svitlana Melnik, Maïwen Caudron-Herger, Lilija Brant, Ian M. Carr, Karsten Rippe, Peter R Cook & Argyris Papantonis

MARC

LEADER 00000caa a2200000 c 4500
001 169722055X
003 DE-627
005 20220818070259.0
007 cr uuu---uuuuu
008 200505s2016 xx |||||o 00| ||eng c
024 7 |a 10.1038/nprot.2016.032  |2 doi 
035 |a (DE-627)169722055X 
035 |a (DE-599)KXP169722055X 
035 |a (OCoLC)1341318614 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Melnik, Svitlana  |e VerfasserIn  |0 (DE-588)118617675X  |0 (DE-627)1665695587  |4 aut 
245 1 0 |a Isolation of the protein and RNA content of active sites of transcription from mammalian cells  |c Svitlana Melnik, Maïwen Caudron-Herger, Lilija Brant, Ian M. Carr, Karsten Rippe, Peter R Cook & Argyris Papantonis 
264 1 |c 25 February 2016 
300 |a 13 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 05.05.2020 
520 |a Transcription factories contain all three mammalian RNA polymerases, each actively transcribing a different subset of genes. This protocol describes how to isolate large factory fragments for the analysis of associated protein and RNA content. 
700 1 |a Caudron-Herger, Maïwen  |e VerfasserIn  |0 (DE-588)1168432464  |0 (DE-627)1032231041  |0 (DE-576)511546106  |4 aut 
700 1 |a Brant, Lilija  |e VerfasserIn  |4 aut 
700 1 |a Carr, Ian M.  |e VerfasserIn  |4 aut 
700 1 |a Rippe, Karsten  |e VerfasserIn  |0 (DE-588)102853261X  |0 (DE-627)730885070  |0 (DE-576)168897989  |4 aut 
700 1 |a Cook, Peter R.  |e VerfasserIn  |4 aut 
700 1 |a Papantonis, Argyris  |e VerfasserIn  |4 aut 
773 0 8 |i Enthalten in  |t Nature protocols  |d Basingstoke : Nature Publishing Group, 2006  |g 11(2016), 3, Seite 553-565  |h Online-Ressource  |w (DE-627)515980552  |w (DE-600)2244966-8  |w (DE-576)276000455  |x 1750-2799  |7 nnas  |a Isolation of the protein and RNA content of active sites of transcription from mammalian cells 
773 1 8 |g volume:11  |g year:2016  |g number:3  |g pages:553-565  |g extent:13  |a Isolation of the protein and RNA content of active sites of transcription from mammalian cells 
856 4 0 |u https://doi.org/10.1038/nprot.2016.032  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.nature.com/articles/nprot.2016.032  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20200505 
993 |a Article 
994 |a 2016 
998 |g 102853261X  |a Rippe, Karsten  |m 102853261X:Rippe, Karsten  |d 700000  |d 716000  |e 700000PR102853261X  |e 716000PR102853261X  |k 0/700000/  |k 1/700000/716000/  |p 5 
998 |g 1168432464  |a Caudron-Herger, Maïwen  |m 1168432464:Caudron-Herger, Maïwen  |d 140000  |d 140001  |d 700000  |d 716000  |e 140000PC1168432464  |e 140001PC1168432464  |e 700000PC1168432464  |e 716000PC1168432464  |k 0/140000/  |k 1/140000/140001/  |k 0/700000/  |k 1/700000/716000/  |p 2 
999 |a KXP-PPN169722055X  |e 3658836008 
BIB |a Y 
SER |a journal 
JSO |a {"recId":"169722055X","origin":[{"dateIssuedKey":"2016","dateIssuedDisp":"25 February 2016"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 05.05.2020"],"relHost":[{"type":{"media":"Online-Ressource","bibl":"periodical"},"titleAlt":[{"title":"Protocols"}],"recId":"515980552","origin":[{"publisher":"Nature Publishing Group","dateIssuedDisp":"2006-","dateIssuedKey":"2006","publisherPlace":"Basingstoke"}],"note":["Gesehen am 18. September 2018"],"id":{"eki":["515980552"],"issn":["1750-2799"],"zdb":["2244966-8"]},"disp":"Isolation of the protein and RNA content of active sites of transcription from mammalian cellsNature protocols","physDesc":[{"extent":"Online-Ressource"}],"part":{"volume":"11","text":"11(2016), 3, Seite 553-565","year":"2016","issue":"3","extent":"13","pages":"553-565"},"language":["eng"],"title":[{"title_sort":"Nature protocols","title":"Nature protocols"}],"pubHistory":["1.2006 -"]}],"physDesc":[{"extent":"13 S."}],"id":{"doi":["10.1038/nprot.2016.032"],"eki":["169722055X"]},"name":{"displayForm":["Svitlana Melnik, Maïwen Caudron-Herger, Lilija Brant, Ian M. Carr, Karsten Rippe, Peter R Cook & Argyris Papantonis"]},"person":[{"family":"Melnik","display":"Melnik, Svitlana","given":"Svitlana","role":"aut"},{"display":"Caudron-Herger, Maïwen","given":"Maïwen","role":"aut","family":"Caudron-Herger"},{"display":"Brant, Lilija","given":"Lilija","role":"aut","family":"Brant"},{"display":"Carr, Ian M.","role":"aut","given":"Ian M.","family":"Carr"},{"family":"Rippe","display":"Rippe, Karsten","role":"aut","given":"Karsten"},{"role":"aut","given":"Peter R.","display":"Cook, Peter R.","family":"Cook"},{"display":"Papantonis, Argyris","role":"aut","given":"Argyris","family":"Papantonis"}],"language":["eng"],"title":[{"title_sort":"Isolation of the protein and RNA content of active sites of transcription from mammalian cells","title":"Isolation of the protein and RNA content of active sites of transcription from mammalian cells"}]} 
SRT |a MELNIKSVITISOLATIONO2520 

Ähnliche Einträge