Zebrafish biosensor for toxicant induced muscle hyperactivity
Robust and sensitive detection systems are a crucial asset for risk management of chemicals, which are produced in increasing number and diversity. To establish an in vivo biosensor system with quantitative readout for potential toxicant effects on motor function, we generated a transgenic zebrafish...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
31 March 2016
|
| In: |
Scientific reports
Year: 2016, Jahrgang: 6 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep23768 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/srep23768 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/srep23768 
|
| Verfasserangaben: | Maryam Shahid, Masanari Takamiya, Johannes Stegmaier, Volker Middel, Marion Gradl, Nils Klüver, Ralf Mikut, Thomas Dickmeis, Stefan Scholz, Sepand Rastegar, Lixin Yang & Uwe Strähle |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1697238793 | ||
| 003 | DE-627 | ||
| 005 | 20220818070733.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200505s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/srep23768 |2 doi | |
| 035 | |a (DE-627)1697238793 | ||
| 035 | |a (DE-599)KXP1697238793 | ||
| 035 | |a (OCoLC)1341318697 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Shahid, Maryam |e VerfasserIn |0 (DE-588)1098545095 |0 (DE-627)857725661 |0 (DE-576)469152192 |4 aut | |
| 245 | 1 | 0 | |a Zebrafish biosensor for toxicant induced muscle hyperactivity |c Maryam Shahid, Masanari Takamiya, Johannes Stegmaier, Volker Middel, Marion Gradl, Nils Klüver, Ralf Mikut, Thomas Dickmeis, Stefan Scholz, Sepand Rastegar, Lixin Yang & Uwe Strähle |
| 264 | 1 | |c 31 March 2016 | |
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 05.05.2020 | ||
| 520 | |a Robust and sensitive detection systems are a crucial asset for risk management of chemicals, which are produced in increasing number and diversity. To establish an in vivo biosensor system with quantitative readout for potential toxicant effects on motor function, we generated a transgenic zebrafish line TgBAC(hspb11:GFP) which expresses a GFP reporter under the control of regulatory elements of the small heat shock protein hspb11. Spatiotemporal hspb11 transgene expression in the musculature and the notochord matched closely that of endogenous hspb11 expression. Exposure to substances that interfere with motor function induced a dose-dependent increase of GFP intensity beginning at sub-micromolar concentrations, while washout of the chemicals reduced the level of hspb11 transgene expression. Simultaneously, these toxicants induced muscle hyperactivity with increased calcium spike height and frequency. The hspb11 transgene up-regulation induced by either chemicals or heat shock was eliminated after co-application of the anaesthetic MS-222. TgBAC(hspb11:GFP) zebrafish embryos provide a quantitative measure of muscle hyperactivity and represent a robust whole organism system for detecting chemicals that affect motor function. | ||
| 700 | 1 | |a Middel, Volker |e VerfasserIn |0 (DE-588)1104176637 |0 (DE-627)861665899 |0 (DE-576)470856637 |4 aut | |
| 700 | 1 | |a Strähle, Uwe |e VerfasserIn |0 (DE-588)106866570X |0 (DE-627)820583634 |0 (DE-576)166301523 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Scientific reports |d [London] : Springer Nature, 2011 |g 6(2016) Artikel-Nummer 23768, 14 Seiten |h Online-Ressource |w (DE-627)663366712 |w (DE-600)2615211-3 |w (DE-576)346641179 |x 2045-2322 |7 nnas |a Zebrafish biosensor for toxicant induced muscle hyperactivity |
| 773 | 1 | 8 | |g volume:6 |g year:2016 |g extent:14 |a Zebrafish biosensor for toxicant induced muscle hyperactivity |
| 856 | 4 | 0 | |u https://doi.org/10.1038/srep23768 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/srep23768 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200505 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 106866570X |a Strähle, Uwe |m 106866570X:Strähle, Uwe |d 140000 |e 140000PS106866570X |k 0/140000/ |p 12 |y j | ||
| 998 | |g 1104176637 |a Middel, Volker |m 1104176637:Middel, Volker |d 140000 |e 140000PM1104176637 |k 0/140000/ |p 4 | ||
| 998 | |g 1098545095 |a Shahid, Maryam |m 1098545095:Shahid, Maryam |d 140000 |e 140000PS1098545095 |k 0/140000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1697238793 |e 3659077631 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"family":"Shahid","role":"aut","given":"Maryam","display":"Shahid, Maryam"},{"family":"Middel","display":"Middel, Volker","given":"Volker","role":"aut"},{"given":"Uwe","role":"aut","display":"Strähle, Uwe","family":"Strähle"}],"name":{"displayForm":["Maryam Shahid, Masanari Takamiya, Johannes Stegmaier, Volker Middel, Marion Gradl, Nils Klüver, Ralf Mikut, Thomas Dickmeis, Stefan Scholz, Sepand Rastegar, Lixin Yang & Uwe Strähle"]},"physDesc":[{"extent":"14 S."}],"id":{"eki":["1697238793"],"doi":["10.1038/srep23768"]},"note":["Gesehen am 05.05.2020"],"relHost":[{"disp":"Zebrafish biosensor for toxicant induced muscle hyperactivityScientific reports","id":{"eki":["663366712"],"zdb":["2615211-3"],"issn":["2045-2322"]},"physDesc":[{"extent":"Online-Ressource"}],"note":["Gesehen am 12.07.24"],"origin":[{"dateIssuedKey":"2011","publisher":"Springer Nature ; Nature Publishing Group","dateIssuedDisp":"2011-","publisherPlace":"[London] ; London"}],"recId":"663366712","type":{"media":"Online-Ressource","bibl":"periodical"},"pubHistory":["1, article number 1 (2011)-"],"title":[{"title":"Scientific reports","title_sort":"Scientific reports"}],"part":{"extent":"14","volume":"6","text":"6(2016) Artikel-Nummer 23768, 14 Seiten","year":"2016"},"language":["eng"]}],"recId":"1697238793","origin":[{"dateIssuedKey":"2016","dateIssuedDisp":"31 March 2016"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"title":[{"title":"Zebrafish biosensor for toxicant induced muscle hyperactivity","title_sort":"Zebrafish biosensor for toxicant induced muscle hyperactivity"}],"language":["eng"]} | ||
| SRT | |a SHAHIDMARYZEBRAFISHB3120 | ||