Proteomic analysis of silenced cathepsin B expression suggests non-proteolytic cathepsin B functionality
Cathepsin B (CTSB) is a lysosomal endo- and exopeptidase that is also secreted in high amounts by malignant and non-malignant cells. We determined the effect of CTSB on the tumor cell secretome by shRNA-mediated silencing of CTSB mRNA expression and subsequent proteomic LC-MS/MS analysis of the cell...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
12 August 2016
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| In: |
Biochimica et biophysica acta. Molecular cell research
Year: 2016, Volume: 1863, Issue: 11, Pages: 2700-2709 |
| ISSN: | 1879-2596 |
| DOI: | 10.1016/j.bbamcr.2016.08.005 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.bbamcr.2016.08.005 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0167488916302105 
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| Author Notes: | Florian Christoph Sigloch, Julia Daniela Knopf, Juliane Weißer, Alejandro Gomez-Auli, Martin Lothar Biniossek, Agnese Petrera, Oliver Schilling |
| Summary: | Cathepsin B (CTSB) is a lysosomal endo- and exopeptidase that is also secreted in high amounts by malignant and non-malignant cells. We determined the effect of CTSB on the tumor cell secretome by shRNA-mediated silencing of CTSB mRNA expression and subsequent proteomic LC-MS/MS analysis of the cell supernatants. We identified significant protein changes of 17 secreted or shed proteins. Notably, we found a general reduction in protein abundance of ADAM10 substrates and lysosomal proteins. We corroborated reduced amounts of soluble ADAM10 (sADAM10) and soluble APP (sAPP) in the two cancer cell lines MDA-MB-231 and U2OS by immunoblotting. Interestingly, reductions in sADAM10 and sAPP could be reversed by re-introducing a catalytically inactive variant of CTSB, suggesting a formerly unknown non-catalytic function of the protease. |
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| Item Description: | Gesehen am 07.05.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1879-2596 |
| DOI: | 10.1016/j.bbamcr.2016.08.005 |