Generation and functional characterization of epithelial cells with stable expression of SLC26A9 Cl− channels
Recent studies identified the SLC26A9 Cl− channel as a modifier and potential therapeutic target in cystic fibrosis (CF). However, understanding of the regulation of SLC26A9 in epithelia remains limited and cellular models with stable expression for biochemical and functional studies are missing. We...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
01 Apr 2016
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| In: |
American journal of physiology. Lung cellular and molecular physiology
Year: 2016, Jahrgang: 310, Heft: 7, Pages: L593-L602 |
| ISSN: | 1522-1504 |
| DOI: | 10.1152/ajplung.00321.2015 |
| Online-Zugang: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1152/ajplung.00321.2015 Verlag, lizenzpflichtig, Volltext: https://journals.physiology.org/doi/full/10.1152/ajplung.00321.2015 
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| Verfasserangaben: | Johanna J. Salomon, Stephan Spahn, Xiaohui Wang, Joachim Füllekrug, Carol A. Bertrand, and Marcus A. Mall |
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| 520 | |a Recent studies identified the SLC26A9 Cl− channel as a modifier and potential therapeutic target in cystic fibrosis (CF). However, understanding of the regulation of SLC26A9 in epithelia remains limited and cellular models with stable expression for biochemical and functional studies are missing. We, therefore, generated Fisher rat thyroid (FRT) epithelial cells with stable expression of HA-tagged SLC26A9 via retroviral transfection and characterized SLC26A9 expression and function using Western blotting, immunolocalization, whole cell patch-clamp, and transepithelial bioelectric studies in Ussing chambers. We demonstrate stable expression of SLC26A9 in transfected FRT (SLC26A9-FRT) cells on the mRNA and protein level. | ||
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