Chemical derivatization and biofunctionalization of hydrogel nanomembranes for potential biomedical and biosensor applications
Poly(ethylene glycol) based hydrogel nanomembranes (PHMs) are demonstrated to be able to host protein-specific receptors, providing, at the same time, stable, protein-repelling matrices with a characteristic mesh size up to 7-8 nm. The membranes were prepared by crosslinking of amino- and epoxy-term...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
[2016]
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| In: |
Physical chemistry, chemical physics
Year: 2016, Volume: 18, Issue: 17, Pages: 12035-12042 |
| ISSN: | 1463-9084 |
| DOI: | 10.1039/C5CP07840G |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1039/C5CP07840G Verlag, lizenzpflichtig, Volltext: https://pubs.rsc.org/en/content/articlelanding/2016/cp/c5cp07840g 
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| Author Notes: | Musammir Khan, Swen Schuster and Michael Zharnikov |
| Summary: | Poly(ethylene glycol) based hydrogel nanomembranes (PHMs) are demonstrated to be able to host protein-specific receptors, providing, at the same time, stable, protein-repelling matrices with a characteristic mesh size up to 7-8 nm. The membranes were prepared by crosslinking of amino- and epoxy-terminated STAR-PEG precursors and maintained their hydrogel and protein-repelling properties even at a deviation of the precursor composition from the equilibrium value (1 : 1). The grafting density of the test avidin protein, specifically attached to the biotin moieties coupled to the free amine groups in the PHMs, varied from 0.45 × 1012 to 1.3 × 1012 proteins per cm2 within the sampling depth of the experiments (∼11.5 nm), depending on the precursor composition, whereas the analogous values for the non-specifically adsorbed proteins were lower by a factor of 4-5. The engineering of PHMs with biomolecule-specific receptors and their loading with biomolecules are of potential interest for sensor fabrication and biomedical applications, including tissue engineering and regenerative therapy. |
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| Item Description: | Gesehen am 11.05.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1463-9084 |
| DOI: | 10.1039/C5CP07840G |