Glutamate metabolism in cerebral mitochondria after ischemia and post-ischemic recovery during aging: relationships with brain energy metabolism
Glutamate is involved in cerebral ischemic injury, but its role has not been completely clarified and studies are required to understand how to minimize its detrimental effects, contemporarily boosting the positive ones. In fact, glutamate is not only a neurotransmitter, but primarily a key metaboli...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
20 May 2018
|
| In: |
Journal of neurochemistry
Year: 2018, Volume: 146, Issue: 4, Pages: 416-428 |
| ISSN: | 1471-4159 |
| DOI: | 10.1111/jnc.14464 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/jnc.14464 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/jnc.14464 
|
| Author Notes: | Federica Ferrari, Antonella Gorini, Siegfried Hoyer and Roberto Federico Villa |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1698513836 | ||
| 003 | DE-627 | ||
| 005 | 20220818091219.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200519s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/jnc.14464 |2 doi | |
| 035 | |a (DE-627)1698513836 | ||
| 035 | |a (DE-599)KXP1698513836 | ||
| 035 | |a (OCoLC)1341325703 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Ferrari, Federica |e VerfasserIn |0 (DE-588)1210493861 |0 (DE-627)1698513828 |4 aut | |
| 245 | 1 | 0 | |a Glutamate metabolism in cerebral mitochondria after ischemia and post-ischemic recovery during aging |b relationships with brain energy metabolism |c Federica Ferrari, Antonella Gorini, Siegfried Hoyer and Roberto Federico Villa |
| 264 | 1 | |c 20 May 2018 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 19.05.2020 | ||
| 520 | |a Glutamate is involved in cerebral ischemic injury, but its role has not been completely clarified and studies are required to understand how to minimize its detrimental effects, contemporarily boosting the positive ones. In fact, glutamate is not only a neurotransmitter, but primarily a key metabolite for brain bioenergetics. Thus, we investigated the relationships between glutamate and brain energy metabolism in an in vivo model of complete cerebral ischemia of 15 min and during post-ischemic recovery after 1, 24, 48, 72, and 96 h in 1-year-old adult and 2-year-old aged rats. The maximum rates (Vmax) of glutamate dehydrogenase (GlDH), glutamate-oxaloacetate transaminase, and glutamate-pyruvate transaminase were assayed in somatic mitochondria (FM) and in intra-synaptic ‘Light’ mitochondria and intra-synaptic ‘Heavy’ mitochondria ones purified from cerebral cortex, distinguishing post- and pre-synaptic compartments. During ischemia, none of the enzymes were modified in adult animals. In aged ones, glutamate-oxaloacetate transaminase was increased in FM and GlDH in intra-synaptic ‘Heavy’ mitochondria, stimulating glutamate catabolism. During post-ischemic recovery, FM did not show modifications at both ages while, in intra-synaptic mitochondria of adult animals, glutamate catabolism was increased after 1 h of recirculation and decreased after 48 and 72 h, whereas it remained decreased up to 96 h in aged rats. These results, with those previously published about Krebs’ cycle and Electron Transport Chain (Villa et al., [2013] Neurochem. Int. 63, 765-781), demonstrate that: (i) Vmax of energy-linked enzymes are different in the various cerebral mitochondria, which (ii) respond differently to ischemia and post-ischemic recovery, also (iii) with respect to aging. | ||
| 650 | 4 | |a aging | |
| 650 | 4 | |a brain energy metabolism | |
| 650 | 4 | |a glutamate | |
| 650 | 4 | |a ischemia | |
| 650 | 4 | |a mitochondria | |
| 650 | 4 | |a recirculation | |
| 700 | 1 | |a Gorini, Antonella |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hoyer, Siegfried |d 1933-2014 |e VerfasserIn |0 (DE-588)133236773 |0 (DE-627)539307343 |0 (DE-576)299715469 |4 aut | |
| 700 | 1 | |a Villa, Roberto Federico |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of neurochemistry |d Oxford : Wiley-Blackwell, 1956 |g 146(2018), 4, Seite 416-428 |h Online-Ressource |w (DE-627)320604063 |w (DE-600)2020528-4 |w (DE-576)091137454 |x 1471-4159 |7 nnas |a Glutamate metabolism in cerebral mitochondria after ischemia and post-ischemic recovery during aging relationships with brain energy metabolism |
| 773 | 1 | 8 | |g volume:146 |g year:2018 |g number:4 |g pages:416-428 |g extent:13 |a Glutamate metabolism in cerebral mitochondria after ischemia and post-ischemic recovery during aging relationships with brain energy metabolism |
| 856 | 4 | 0 | |u https://doi.org/10.1111/jnc.14464 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://onlinelibrary.wiley.com/doi/abs/10.1111/jnc.14464 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200519 | ||
| 993 | |a Article | ||
| 994 | |a 2018 | ||
| 998 | |g 133236773 |a Hoyer, Siegfried |m 133236773:Hoyer, Siegfried |d 910000 |d 912000 |e 910000PH133236773 |e 912000PH133236773 |k 0/910000/ |k 1/910000/912000/ |p 3 | ||
| 999 | |a KXP-PPN1698513836 |e 3668485186 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"subtitle":"relationships with brain energy metabolism","title":"Glutamate metabolism in cerebral mitochondria after ischemia and post-ischemic recovery during aging","title_sort":"Glutamate metabolism in cerebral mitochondria after ischemia and post-ischemic recovery during aging"}],"person":[{"display":"Ferrari, Federica","roleDisplay":"VerfasserIn","role":"aut","family":"Ferrari","given":"Federica"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Gorini, Antonella","given":"Antonella","family":"Gorini"},{"given":"Siegfried","family":"Hoyer","role":"aut","display":"Hoyer, Siegfried","roleDisplay":"VerfasserIn"},{"given":"Roberto Federico","family":"Villa","role":"aut","roleDisplay":"VerfasserIn","display":"Villa, Roberto Federico"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 19.05.2020"],"language":["eng"],"recId":"1698513836","origin":[{"dateIssuedDisp":"20 May 2018","dateIssuedKey":"2018"}],"id":{"doi":["10.1111/jnc.14464"],"eki":["1698513836"]},"name":{"displayForm":["Federica Ferrari, Antonella Gorini, Siegfried Hoyer and Roberto Federico Villa"]},"physDesc":[{"extent":"13 S."}],"relHost":[{"pubHistory":["1.1956/57 -"],"titleAlt":[{"title":"JNC"}],"part":{"extent":"13","text":"146(2018), 4, Seite 416-428","volume":"146","issue":"4","pages":"416-428","year":"2018"},"disp":"Glutamate metabolism in cerebral mitochondria after ischemia and post-ischemic recovery during aging relationships with brain energy metabolismJournal of neurochemistry","note":["Fortsetzung der Druck-Ausgabe","Gesehen am 08.04.22"],"type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"320604063","corporate":[{"display":"International Society for Neurochemistry","roleDisplay":"Herausgebendes Organ","role":"isb"}],"language":["eng"],"title":[{"subtitle":"official journal of the International Society for Neurochemistry ; JNC","title":"Journal of neurochemistry","title_sort":"Journal of neurochemistry"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisherPlace":"Oxford ; Stanford, Calif. ; Oxford [u.a.]","publisher":"Wiley-Blackwell ; HighWire Press ; Blackwell","dateIssuedKey":"1956","dateIssuedDisp":"1956-"}],"id":{"zdb":["2020528-4"],"eki":["320604063"],"doi":["10.1111/(ISSN)1471-4159"],"issn":["1471-4159"]}}]} | ||
| SRT | |a FERRARIFEDGLUTAMATEM2020 | ||