Mitochondrial redox and pH signaling occurs in axonal and synaptic organelle clusters
Redox switches are important mediators in neoplastic, cardiovascular and neurological disorders. We recently identified spontaneous redox signals in neurons at the single mitochondrion level where transients of glutathione oxidation go along with shortening and re-elongation of the organelle. We now...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
22 March 2016
|
| In: |
Scientific reports
Year: 2016, Volume: 6 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep23251 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/srep23251 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/srep23251 
|
| Author Notes: | Michael O. Breckwoldt, Antonis A. Armoundas, Miguel A. Aon, Martin Bendszus, Brian O’Rourke, Markus Schwarzländer, Tobias P. Dick, Felix T. Kurz |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1698522290 | ||
| 003 | DE-627 | ||
| 005 | 20220818091501.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200519s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/srep23251 |2 doi | |
| 035 | |a (DE-627)1698522290 | ||
| 035 | |a (DE-599)KXP1698522290 | ||
| 035 | |a (OCoLC)1341325731 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Breckwoldt, Michael O. |d 1983- |e VerfasserIn |0 (DE-588)1022496298 |0 (DE-627)716993392 |0 (DE-576)365498297 |4 aut | |
| 245 | 1 | 0 | |a Mitochondrial redox and pH signaling occurs in axonal and synaptic organelle clusters |c Michael O. Breckwoldt, Antonis A. Armoundas, Miguel A. Aon, Martin Bendszus, Brian O’Rourke, Markus Schwarzländer, Tobias P. Dick, Felix T. Kurz |
| 264 | 1 | |c 22 March 2016 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 19.05.2020 | ||
| 520 | |a Redox switches are important mediators in neoplastic, cardiovascular and neurological disorders. We recently identified spontaneous redox signals in neurons at the single mitochondrion level where transients of glutathione oxidation go along with shortening and re-elongation of the organelle. We now have developed advanced image and signal-processing methods to re-assess and extend previously obtained data. Here we analyze redox and pH signals of entire mitochondrial populations. In total, we quantified the effects of 628 redox and pH events in 1797 mitochondria from intercostal axons and neuromuscular synapses using optical sensors (mito-Grx1-roGFP2; mito-SypHer). We show that neuronal mitochondria can undergo multiple redox cycles exhibiting markedly different signal characteristics compared to single redox events. Redox and pH events occur more often in mitochondrial clusters (medium cluster size: 34.1 ± 4.8 μm2). Local clusters possess higher mitochondrial densities than the rest of the axon, suggesting morphological and functional inter-mitochondrial coupling. We find that cluster formation is redox sensitive and can be blocked by the antioxidant MitoQ. In a nerve crush paradigm, mitochondrial clusters form sequentially adjacent to the lesion site and oxidation spreads between mitochondria. Our methodology combines optical bioenergetics and advanced signal processing and allows quantitative assessment of entire mitochondrial populations. | ||
| 700 | 1 | |a Armoundas, Antonis A. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Aon, Miguel A. |e VerfasserIn |0 (DE-588)1158407149 |0 (DE-627)1022062883 |0 (DE-576)503948721 |4 aut | |
| 700 | 1 | |a Bendszus, Martin |e VerfasserIn |0 (DE-588)1032676426 |0 (DE-627)738634131 |0 (DE-576)175567697 |4 aut | |
| 700 | 1 | |a O’Rourke, Brian |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schwarzländer, Markus |e VerfasserIn |4 aut | |
| 700 | 1 | |a Dick, Tobias P. |e VerfasserIn |0 (DE-588)1142415805 |0 (DE-627)100217807X |0 (DE-576)494813431 |4 aut | |
| 700 | 1 | |a Kurz, Felix T. |d 1981- |e VerfasserIn |0 (DE-588)1043285474 |0 (DE-627)771321066 |0 (DE-576)394610342 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Scientific reports |d [London] : Springer Nature, 2011 |g 6(2016) Artikel-Nummer 23251, 12 Seiten |h Online-Ressource |w (DE-627)663366712 |w (DE-600)2615211-3 |w (DE-576)346641179 |x 2045-2322 |7 nnas |a Mitochondrial redox and pH signaling occurs in axonal and synaptic organelle clusters |
| 773 | 1 | 8 | |g volume:6 |g year:2016 |g extent:12 |a Mitochondrial redox and pH signaling occurs in axonal and synaptic organelle clusters |
| 856 | 4 | 0 | |u https://doi.org/10.1038/srep23251 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/srep23251 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200519 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 1043285474 |a Kurz, Felix T. |m 1043285474:Kurz, Felix T. |d 910000 |d 911100 |e 910000PK1043285474 |e 911100PK1043285474 |k 0/910000/ |k 1/910000/911100/ |p 8 |y j | ||
| 998 | |g 1142415805 |a Dick, Tobias P. |m 1142415805:Dick, Tobias P. |d 140000 |e 140000PD1142415805 |k 0/140000/ |p 7 | ||
| 998 | |g 1032676426 |a Bendszus, Martin |m 1032676426:Bendszus, Martin |d 910000 |d 911100 |e 910000PB1032676426 |e 911100PB1032676426 |k 0/910000/ |k 1/910000/911100/ |p 4 | ||
| 998 | |g 1022496298 |a Breckwoldt, Michael O. |m 1022496298:Breckwoldt, Michael O. |d 910000 |d 911100 |e 910000PB1022496298 |e 911100PB1022496298 |k 0/910000/ |k 1/910000/911100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1698522290 |e 3668502218 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"doi":["10.1038/srep23251"],"eki":["1698522290"]},"recId":"1698522290","type":{"bibl":"article-journal","media":"Online-Ressource"},"name":{"displayForm":["Michael O. Breckwoldt, Antonis A. Armoundas, Miguel A. Aon, Martin Bendszus, Brian O’Rourke, Markus Schwarzländer, Tobias P. Dick, Felix T. Kurz"]},"language":["eng"],"physDesc":[{"extent":"12 S."}],"relHost":[{"title":[{"title_sort":"Scientific reports","title":"Scientific reports"}],"part":{"year":"2016","volume":"6","extent":"12","text":"6(2016) Artikel-Nummer 23251, 12 Seiten"},"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Mitochondrial redox and pH signaling occurs in axonal and synaptic organelle clustersScientific reports","language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"note":["Gesehen am 12.07.24"],"origin":[{"publisherPlace":"[London] ; London","dateIssuedKey":"2011","dateIssuedDisp":"2011-","publisher":"Springer Nature ; Nature Publishing Group"}],"id":{"zdb":["2615211-3"],"eki":["663366712"],"issn":["2045-2322"]},"pubHistory":["1, article number 1 (2011)-"],"recId":"663366712"}],"title":[{"title_sort":"Mitochondrial redox and pH signaling occurs in axonal and synaptic organelle clusters","title":"Mitochondrial redox and pH signaling occurs in axonal and synaptic organelle clusters"}],"person":[{"role":"aut","display":"Breckwoldt, Michael O.","given":"Michael O.","family":"Breckwoldt"},{"family":"Armoundas","given":"Antonis A.","display":"Armoundas, Antonis A.","role":"aut"},{"display":"Aon, Miguel A.","role":"aut","given":"Miguel A.","family":"Aon"},{"role":"aut","display":"Bendszus, Martin","given":"Martin","family":"Bendszus"},{"family":"O’Rourke","given":"Brian","display":"O’Rourke, Brian","role":"aut"},{"given":"Markus","family":"Schwarzländer","role":"aut","display":"Schwarzländer, Markus"},{"display":"Dick, Tobias P.","role":"aut","given":"Tobias P.","family":"Dick"},{"given":"Felix T.","family":"Kurz","display":"Kurz, Felix T.","role":"aut"}],"origin":[{"dateIssuedDisp":"22 March 2016","dateIssuedKey":"2016"}],"note":["Gesehen am 19.05.2020"]} | ||
| SRT | |a BRECKWOLDTMITOCHONDR2220 | ||