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Architecture of the 90S pre-ribosome: a structural view on the birth of the eukaryotic ribosome

The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of ∼70 assembly factors and several small nucleolar RNAs (snoRNAs) that associate with nascent pre-rRNA. We report the cryo-EM structure of the Chaetomium thermophilum 90S pre-ribos...

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Main Authors: Kornprobst, Markus (Author) , Turk, Martin (Author) , Kellner, Nikola (Author) , Cheng, Jingdong (Author) , Flemming, Dirk (Author) , Koš-Braun, Isabelle C. (Author) , Koš, Martin (Author) , Thoms, Matthias (Author) , Berninghausen, Otto (Author) , Beckmann, Roland (Author) , Hurt, Ed (Author)
Format: Article (Journal)
Language:English
Published: 14 July 2016
In: Cell
Year: 2016, Volume: 166, Issue: 2, Pages: 380-393
ISSN:1097-4172
DOI:10.1016/j.cell.2016.06.014
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.cell.2016.06.014
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0092867416307437
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Author Notes:Markus Kornprobst, Martin Turk, Nikola Kellner, Jingdong Cheng, Dirk Flemming, Isabelle Koš-Braun, Martin Koš, Matthias Thoms, Otto Berninghausen, Roland Beckmann, and Ed Hurt
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Summary:The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of ∼70 assembly factors and several small nucleolar RNAs (snoRNAs) that associate with nascent pre-rRNA. We report the cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome, revealing how a network of biogenesis factors including 19 β-propellers and large α-solenoid proteins engulfs the pre-rRNA. Within the 90S pre-ribosome, we identify the UTP-A, UTP-B, Mpp10-Imp3-Imp4, Bms1-Rcl1, and U3 snoRNP modules, which are organized around 5′-ETS and partially folded 18S rRNA. The U3 snoRNP is strategically positioned at the center of the 90S particle to perform its multiple tasks during pre-rRNA folding and processing. The architecture of the elusive 90S pre-ribosome gives unprecedented structural insight into the early steps of pre-rRNA maturation. Nascent rRNA that is co-transcriptionally folded and given a particular shape by encapsulation within a dedicated mold-like structure is reminiscent of how polypeptides use chaperone chambers for their protein folding.
Item Description:Gesehen am 19.05.2020
Physical Description:Online Resource
ISSN:1097-4172
DOI:10.1016/j.cell.2016.06.014

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