Stemness genes expression in naive vs. osteodifferentiated human dental-derived stem cells
OBJECTIVE: Mesenchymal stem cells (MSCs) have been deeply investigated in regenerative medicine because of their crucial role in tissue healing, such as tissue regeneration. Dental-derived stem cells (d-DSCs) are easily available from dental tissues, which can be isolated from all age patients with...
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| Auteurs principaux: | , , , , , , , , , , , , , , , |
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| Format: | Article (Journal) |
| Langue: | anglais |
| Publié: |
2019
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| In: |
European review for medical and pharmacological sciences
Year: 2019, Volume: 23, Numéro: 7, Pages: 2916-2923 |
| ISSN: | 2284-0729 |
| DOI: | 10.26355/eurrev_201904_17570 |
| Accès en ligne: | Verlag, Volltext: https://doi.org/10.26355/eurrev_201904_17570
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| Notes sur l'auteur: | A. Ballini, A. di Benedetto, D. de Vito, A. Scarano, S. Scacco, L. Perillo, F. Posa, G. Dipalma, F. Paduano, M. Contaldo, M. Grano, G. Brunetti, G. Colaianni, M. di Cosola, S. Cantore, G. Mori |
| Résumé: | OBJECTIVE: Mesenchymal stem cells (MSCs) have been deeply investigated in regenerative medicine because of their crucial role in tissue healing, such as tissue regeneration. Dental-derived stem cells (d-DSCs) are easily available from dental tissues, which can be isolated from all age patients with minimal discomfort. PATIENTS AND METHODS: Normal unerupted third molars tooth buds were collected from adolescents' patients underwent to extractions for orthodontic reasons. The expression of the genes Kruppel-like factor 4 (Klf-4), octamer- binding transcription factor 4 (Oct-4), homeobox transcription factor Nanog (NANOG) was investigated in d-DSCs obtained from dental bud (DBSCs), differentiated toward osteoblastic phenotype and not. RESULTS: Our results showed that DBSCs expressed Oct-4, Nanog, and Klf-4 in undifferentiated conditions and interestingly the expression of such genes increased when the cells were kept in osteogenic medium. CONCLUSIONS: These attractive stemness properties, together with the effortlessly isolation, during common oral and maxillofacial surgical procedures, from undifferentiated tissues such as dental bud, make this kind of d-DSCs a promising tool in regenerative medicine, having the potential for clinical applications, and reinforcing the present challenge to develop new preventive and healing strategies in tissue regeneration. |
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| Description: | Gesehen am 19.05.2020 |
| Description matérielle: | Online Resource |
| ISSN: | 2284-0729 |
| DOI: | 10.26355/eurrev_201904_17570 |