Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolution
Second harmonic generation (SHG) microscopy is a powerful tool for label free ex vivo or in vivo imaging, widely used to investigate structure and organization of endogenous SHG emitting proteins such as myosin or collagen. Polarization resolved SHG microscopy renders supplementary information and i...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
15 Jan 2016
|
| In: |
Biomedical optics express
Year: 2016, Volume: 7, Issue: 2, Pages: 525-541 |
| ISSN: | 2156-7085 |
| DOI: | 10.1364/BOE.7.000525 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1364/BOE.7.000525 Verlag, lizenzpflichtig, Volltext: https://www.osapublishing.org/boe/abstract.cfm?uri=boe-7-2-525 
|
| Author Notes: | Moritz Förderer, Tihomir Georgiev, Matias Mosqueira, Rainer H.A. Fink, and Martin Vogel |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 169860596X | ||
| 003 | DE-627 | ||
| 005 | 20230614131504.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200520s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1364/BOE.7.000525 |2 doi | |
| 035 | |a (DE-627)169860596X | ||
| 035 | |a (DE-599)KXP169860596X | ||
| 035 | |a (OCoLC)1341325806 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Förderer, Moritz |e VerfasserIn |0 (DE-588)1113033959 |0 (DE-627)867046759 |0 (DE-576)330089226 |4 aut | |
| 245 | 1 | 0 | |a Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolution |c Moritz Förderer, Tihomir Georgiev, Matias Mosqueira, Rainer H.A. Fink, and Martin Vogel |
| 264 | 1 | |c 15 Jan 2016 | |
| 300 | |a 17 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 20.05.2020 | ||
| 520 | |a Second harmonic generation (SHG) microscopy is a powerful tool for label free ex vivo or in vivo imaging, widely used to investigate structure and organization of endogenous SHG emitting proteins such as myosin or collagen. Polarization resolved SHG microscopy renders supplementary information and is used to probe different molecular states. This development towards functional SHG microscopy is calling for new methods for high speed functional imaging of dynamic processes. In this work we present two approaches with linear polarized light and demonstrate high speed line scan measurements of the molecular dynamics of the motor protein myosin with a time resolution of 1 ms in mammalian muscle cells. Such a high speed functional SHG microscopy has high potential to deliver new insights into structural and temporal molecular dynamics under ex vivo or in vivo conditions. | ||
| 650 | 4 | |a Circular polarization | |
| 650 | 4 | |a Image processing | |
| 650 | 4 | |a Liquid crystal modulators | |
| 650 | 4 | |a Polarization control | |
| 650 | 4 | |a Polarized light | |
| 650 | 4 | |a Temporal resolution | |
| 700 | 1 | |a Georgiev, Tihomir |e VerfasserIn |0 (DE-588)108225780X |0 (DE-627)847263401 |0 (DE-576)45529528X |4 aut | |
| 700 | 1 | |a Mosqueira, Matias |e VerfasserIn |0 (DE-588)115123348X |0 (DE-627)1011422344 |0 (DE-576)497572494 |4 aut | |
| 700 | 1 | |a Fink, Rainer |d 1949- |e VerfasserIn |0 (DE-588)131973762 |0 (DE-627)707796466 |0 (DE-576)298870142 |4 aut | |
| 700 | 1 | |a Vogel, Martin |e VerfasserIn |0 (DE-588)1212596129 |0 (DE-627)1702121208 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biomedical optics express |d Washington, DC : Optica, 2010 |g 7(2016), 2, Seite 525-541 |h Online-Ressource |w (DE-627)635133415 |w (DE-600)2572216-5 |w (DE-576)330122096 |x 2156-7085 |7 nnas |a Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolution |
| 773 | 1 | 8 | |g volume:7 |g year:2016 |g number:2 |g pages:525-541 |g extent:17 |a Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolution |
| 856 | 4 | 0 | |u https://doi.org/10.1364/BOE.7.000525 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.osapublishing.org/boe/abstract.cfm?uri=boe-7-2-525 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200520 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 1212596129 |a Vogel, Martin |m 1212596129:Vogel, Martin |d 140000 |e 140000PV1212596129 |k 0/140000/ |p 5 |y j | ||
| 998 | |g 131973762 |a Fink, Rainer |m 131973762:Fink, Rainer |d 50000 |d 53200 |e 50000PF131973762 |e 53200PF131973762 |k 0/50000/ |k 1/50000/53200/ |p 4 | ||
| 998 | |g 115123348X |a Mosqueira, Matias |m 115123348X:Mosqueira, Matias |d 50000 |d 53200 |e 50000PM115123348X |e 53200PM115123348X |k 0/50000/ |k 1/50000/53200/ |p 3 | ||
| 998 | |g 108225780X |a Georgiev, Tihomir |m 108225780X:Georgiev, Tihomir |p 2 | ||
| 998 | |g 1113033959 |a Förderer, Moritz |m 1113033959:Förderer, Moritz |p 1 |x j | ||
| 999 | |a KXP-PPN169860596X |e 3668764735 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 20.05.2020"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"recId":"169860596X","title":[{"title":"Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolution","title_sort":"Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolution"}],"person":[{"family":"Förderer","given":"Moritz","display":"Förderer, Moritz","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Georgiev","given":"Tihomir","display":"Georgiev, Tihomir","roleDisplay":"VerfasserIn","role":"aut"},{"display":"Mosqueira, Matias","roleDisplay":"VerfasserIn","role":"aut","family":"Mosqueira","given":"Matias"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Fink, Rainer","given":"Rainer","family":"Fink"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Vogel, Martin","given":"Martin","family":"Vogel"}],"physDesc":[{"extent":"17 S."}],"relHost":[{"pubHistory":["1.2010 -"],"part":{"text":"7(2016), 2, Seite 525-541","volume":"7","extent":"17","year":"2016","issue":"2","pages":"525-541"},"disp":"Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolutionBiomedical optics express","type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 10.01.2022","Herausgebendes Organ bis 2021: Optical Society of America"],"corporate":[{"roleDisplay":"Herausgebendes Organ","display":"Optica","role":"isb"},{"roleDisplay":"Herausgebendes Organ","display":"Optical Society of America","role":"isb"}],"language":["eng"],"recId":"635133415","title":[{"title_sort":"Biomedical optics express","title":"Biomedical optics express"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisherPlace":"Washington, DC ; Washington, DC","dateIssuedDisp":"2010-","publisher":"Optica ; Optical Society of America, OSA","dateIssuedKey":"2010"}],"id":{"issn":["2156-7085"],"zdb":["2572216-5"],"eki":["635133415"]},"name":{"displayForm":["Optica"]}}],"origin":[{"dateIssuedKey":"2016","dateIssuedDisp":"15 Jan 2016"}],"id":{"doi":["10.1364/BOE.7.000525"],"eki":["169860596X"]},"name":{"displayForm":["Moritz Förderer, Tihomir Georgiev, Matias Mosqueira, Rainer H.A. Fink, and Martin Vogel"]}} | ||
| SRT | |a FOERDERERMFUNCTIONAL1520 | ||