miR-8-3p regulates mitoferrin in the testes of Bactrocera dorsalis to ensure normal spermatogenesis
Genetics-enhanced sterile insect techniques (SIT) are promising novel approaches to control Bactrocera dorsalis, the most destructive horticultural pest in East Asia and the Pacific region. To identify novel genetic agents to alter male fertility of B. dorsalis, previous studies investigated miRNA e...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
02 March 2016
|
| In: |
Scientific reports
Year: 2016, Jahrgang: 6 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep22565 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/srep22565 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/srep22565 
|
| Verfasserangaben: | Kaleem Tariq, Christoph Metzendorf, Wei Peng, Summar Sohail, and Hongyu Zhang |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1698613652 | ||
| 003 | DE-627 | ||
| 005 | 20220818092834.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200520s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/srep22565 |2 doi | |
| 035 | |a (DE-627)1698613652 | ||
| 035 | |a (DE-599)KXP1698613652 | ||
| 035 | |a (OCoLC)1341325833 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Tariq, Kaleem |e VerfasserIn |0 (DE-588)1210563746 |0 (DE-627)1698614446 |4 aut | |
| 245 | 1 | 0 | |a miR-8-3p regulates mitoferrin in the testes of Bactrocera dorsalis to ensure normal spermatogenesis |c Kaleem Tariq, Christoph Metzendorf, Wei Peng, Summar Sohail, and Hongyu Zhang |
| 264 | 1 | |c 02 March 2016 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 20.5.2020 | ||
| 520 | |a Genetics-enhanced sterile insect techniques (SIT) are promising novel approaches to control Bactrocera dorsalis, the most destructive horticultural pest in East Asia and the Pacific region. To identify novel genetic agents to alter male fertility of B. dorsalis, previous studies investigated miRNA expression in testes of B. dorsalis. One miRNA, miR-8-3p was predicted to bind the 3′UTR of putative B. dorsalis mitoferrin (bmfrn). The ortholog of bmfrn in D. melanogaster is essential for male fertility. Here we show that bmfrn has all conserved amino acid residues of known mitoferrins and is most abundantly expressed in B. dorsalis testes, making miR-8-3p and mitoferrin candidates for genetics-enhanced SIT. Furthermore, using a dual-luciferase reporter system, we show in HeLa cells that miR-8-3p interacts with the 3′UTR of bmfrn. Dietary treatments of adult male flies with miR-8-3p mimic, antagomiR, or bmfrn dsRNA, altered mitoferrin expression in the testes and resulted in reduced male reproductive capacity due to reduced numbers and viability of spermatozoa. We show for the first time that a mitoferrin is regulated by a miRNA and we demonstrate miR-8-3p as well as bmfrn dsRNA to be promising novel agents that could be used for genetics-enhanced SIT. | ||
| 700 | 1 | |a Metzendorf, Christoph |e VerfasserIn |0 (DE-588)1149853387 |0 (DE-627)1010157884 |0 (DE-576)496855964 |4 aut | |
| 700 | 1 | |a Peng, Wei |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sohail, Summar |e VerfasserIn |4 aut | |
| 700 | 1 | |a Zhang, Hongyu |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Scientific reports |d [London] : Springer Nature, 2011 |g 6(2016) Artikel-Nummer 22565, 9 Seiten |h Online-Ressource |w (DE-627)663366712 |w (DE-600)2615211-3 |w (DE-576)346641179 |x 2045-2322 |7 nnas |a miR-8-3p regulates mitoferrin in the testes of Bactrocera dorsalis to ensure normal spermatogenesis |
| 773 | 1 | 8 | |g volume:6 |g year:2016 |g extent:9 |a miR-8-3p regulates mitoferrin in the testes of Bactrocera dorsalis to ensure normal spermatogenesis |
| 856 | 4 | 0 | |u https://doi.org/10.1038/srep22565 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/srep22565 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200520 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 1149853387 |a Metzendorf, Christoph |m 1149853387:Metzendorf, Christoph |d 700000 |d 711000 |e 700000PM1149853387 |e 711000PM1149853387 |k 0/700000/ |k 1/700000/711000/ |p 2 | ||
| 999 | |a KXP-PPN1698613652 |e 3668779627 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title_sort":"miR-8-3p regulates mitoferrin in the testes of Bactrocera dorsalis to ensure normal spermatogenesis","title":"miR-8-3p regulates mitoferrin in the testes of Bactrocera dorsalis to ensure normal spermatogenesis"}],"person":[{"display":"Tariq, Kaleem","roleDisplay":"VerfasserIn","role":"aut","family":"Tariq","given":"Kaleem"},{"family":"Metzendorf","given":"Christoph","display":"Metzendorf, Christoph","roleDisplay":"VerfasserIn","role":"aut"},{"display":"Peng, Wei","roleDisplay":"VerfasserIn","role":"aut","family":"Peng","given":"Wei"},{"family":"Sohail","given":"Summar","display":"Sohail, Summar","roleDisplay":"VerfasserIn","role":"aut"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Zhang, Hongyu","given":"Hongyu","family":"Zhang"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 20.5.2020"],"recId":"1698613652","language":["eng"],"origin":[{"dateIssuedKey":"2016","dateIssuedDisp":"02 March 2016"}],"id":{"doi":["10.1038/srep22565"],"eki":["1698613652"]},"name":{"displayForm":["Kaleem Tariq, Christoph Metzendorf, Wei Peng, Summar Sohail, and Hongyu Zhang"]},"physDesc":[{"extent":"9 S."}],"relHost":[{"title":[{"title_sort":"Scientific reports","title":"Scientific reports"}],"pubHistory":["1, article number 1 (2011)-"],"part":{"year":"2016","extent":"9","text":"6(2016) Artikel-Nummer 22565, 9 Seiten","volume":"6"},"type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 12.07.24"],"disp":"miR-8-3p regulates mitoferrin in the testes of Bactrocera dorsalis to ensure normal spermatogenesisScientific reports","recId":"663366712","language":["eng"],"origin":[{"publisherPlace":"[London] ; London","publisher":"Springer Nature ; Nature Publishing Group","dateIssuedKey":"2011","dateIssuedDisp":"2011-"}],"id":{"issn":["2045-2322"],"zdb":["2615211-3"],"eki":["663366712"]},"physDesc":[{"extent":"Online-Ressource"}]}]} | ||
| SRT | |a TARIQKALEEMIR83PREGU0220 | ||