Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT
We present a plane-scanning RESOLFT [reversible saturable/switchable optical (fluorescence) transitions] light-sheet (LS) nanoscope, which fundamentally overcomes the diffraction barrier in the axial direction via confinement of the fluorescent molecular state to a sheet of subdiffraction thickness...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
March 16, 2016
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| In: |
Proceedings of the National Academy of Sciences of the United States of America
Year: 2016, Volume: 113, Issue: 13, Pages: 3442-3446 |
| ISSN: | 1091-6490 |
| DOI: | 10.1073/pnas.1522292113 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1073/pnas.1522292113 Verlag, lizenzpflichtig, Volltext: https://www.pnas.org/content/113/13/3442 
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| Author Notes: | Patrick Hoyer, Gustavo de Medeiros, Bálint Balázs, Nils Norlin, Christina Besir, Janina Hanne, Hans-Georg Kräusslich, Johann Engelhardt, Steffen J. Sahl, Stefan W. Hell, Lars Hufnagel |
MARC
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| 245 | 1 | 0 | |a Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT |c Patrick Hoyer, Gustavo de Medeiros, Bálint Balázs, Nils Norlin, Christina Besir, Janina Hanne, Hans-Georg Kräusslich, Johann Engelhardt, Steffen J. Sahl, Stefan W. Hell, Lars Hufnagel |
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| 520 | |a We present a plane-scanning RESOLFT [reversible saturable/switchable optical (fluorescence) transitions] light-sheet (LS) nanoscope, which fundamentally overcomes the diffraction barrier in the axial direction via confinement of the fluorescent molecular state to a sheet of subdiffraction thickness around the focal plane. To this end, reversibly switchable fluorophores located right above and below the focal plane are transferred to a nonfluorescent state at each scanning step. LS-RESOLFT nanoscopy offers wide-field 3D imaging of living biological specimens with low light dose and axial resolution far beyond the diffraction barrier. We demonstrate optical sections that are thinner by 5-12-fold compared with their conventional diffraction-limited LS analogs. | ||
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