Frequent mechanical stress suppresses proliferation of mesenchymal stem cells from human bone marrow without loss of multipotency
Mounting evidence indicated that human mesenchymal stem cells (hMSCs) are responsive not only to biochemical but also to physical cues, such as substrate topography and stiffness. To simulate the dynamic structures of extracellular environments of the marrow in vivo, we designed a novel surrogate su...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
15 April 2016
|
| In: |
Scientific reports
Year: 2016, Volume: 6 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep24264 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/srep24264 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/srep24264 
|
| Author Notes: | Viktoria Frank, Stefan Kaufmann, Rebecca Wright, Patrick Horn, Hiroshi Y. Yoshikawa, Patrick Wuchter, Jeppe Madsen, Andrew L. Lewis, Steven P. Armes, Anthony D. Ho & Motomu Tanaka |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1698678223 | ||
| 003 | DE-627 | ||
| 005 | 20230427091725.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200522s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/srep24264 |2 doi | |
| 035 | |a (DE-627)1698678223 | ||
| 035 | |a (DE-599)KXP1698678223 | ||
| 035 | |a (OCoLC)1341326098 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Frank, Viktoria |e VerfasserIn |0 (DE-588)1151543357 |0 (DE-627)1011905477 |0 (DE-576)497871394 |4 aut | |
| 245 | 1 | 0 | |a Frequent mechanical stress suppresses proliferation of mesenchymal stem cells from human bone marrow without loss of multipotency |c Viktoria Frank, Stefan Kaufmann, Rebecca Wright, Patrick Horn, Hiroshi Y. Yoshikawa, Patrick Wuchter, Jeppe Madsen, Andrew L. Lewis, Steven P. Armes, Anthony D. Ho & Motomu Tanaka |
| 264 | 1 | |c 15 April 2016 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 22.05.2020 | ||
| 520 | |a Mounting evidence indicated that human mesenchymal stem cells (hMSCs) are responsive not only to biochemical but also to physical cues, such as substrate topography and stiffness. To simulate the dynamic structures of extracellular environments of the marrow in vivo, we designed a novel surrogate substrate for marrow derived hMSCs based on physically cross-linked hydrogels whose elasticity can be adopted dynamically by chemical stimuli. Under frequent mechanical stress, hMSCs grown on our hydrogel substrates maintain the expression of STRO-1 over 20 d, irrespective of the substrate elasticity. On exposure to the corresponding induction media, these cultured hMSCs can undergo adipogenesis and osteogenesis without requiring cell transfer onto other substrates. Moreover, we demonstrated that our surrogate substrate suppresses the proliferation of hMSCs by up to 90% without any loss of multiple lineage potential by changing the substrate elasticity every 2nd days. Such “dynamic in vitro niche” can be used not only for a better understanding of the role of dynamic mechanical stresses on the fate of hMSCs but also for the synchronized differentiation of adult stem cells to a specific lineage. | ||
| 700 | 1 | |a Kaufmann, Stefan |d 1948- |e VerfasserIn |0 (DE-588)1060647400 |0 (DE-627)800412656 |0 (DE-576)416771424 |4 aut | |
| 700 | 1 | |a Wright, Rebecca |e VerfasserIn |0 (DE-588)1210715538 |0 (DE-627)1698680961 |4 aut | |
| 700 | 1 | |a Horn, Patrick |e VerfasserIn |0 (DE-588)143037390 |0 (DE-627)704371251 |0 (DE-576)334708311 |4 aut | |
| 700 | 1 | |a Yoshikawa, Hiroshi Y. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wuchter, Patrick |d 1975- |e VerfasserIn |0 (DE-588)123450284 |0 (DE-627)082563624 |0 (DE-576)184642574 |4 aut | |
| 700 | 1 | |a Madsen, Jeppe |e VerfasserIn |4 aut | |
| 700 | 1 | |a Lewis, Andrew L. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Armes, Steven P. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ho, Anthony Dick |d 1948- |e VerfasserIn |0 (DE-588)108692477 |0 (DE-627)504904205 |0 (DE-576)19010466X |4 aut | |
| 700 | 1 | |a Tanaka, Motomu |e VerfasserIn |0 (DE-588)1060648377 |0 (DE-627)800414829 |0 (DE-576)416774636 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Scientific reports |d [London] : Springer Nature, 2011 |g 6(2016) Artikel-Nummer 24264, 12 Seiten |h Online-Ressource |w (DE-627)663366712 |w (DE-600)2615211-3 |w (DE-576)346641179 |x 2045-2322 |7 nnas |a Frequent mechanical stress suppresses proliferation of mesenchymal stem cells from human bone marrow without loss of multipotency |
| 773 | 1 | 8 | |g volume:6 |g year:2016 |g extent:12 |a Frequent mechanical stress suppresses proliferation of mesenchymal stem cells from human bone marrow without loss of multipotency |
| 856 | 4 | 0 | |u https://doi.org/10.1038/srep24264 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/srep24264 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200522 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 1210715538 |a Wright, Rebecca |m 1210715538:Wright, Rebecca |d 700000 |d 716000 |e 700000PW1210715538 |e 716000PW1210715538 |k 0/700000/ |k 1/700000/716000/ |p 3 | ||
| 998 | |g 1060648377 |a Tanaka, Motomu |m 1060648377:Tanaka, Motomu |d 120000 |d 120300 |e 120000PT1060648377 |e 120300PT1060648377 |k 0/120000/ |k 1/120000/120300/ |p 12 | ||
| 998 | |g 108692477 |a Ho, Anthony Dick |m 108692477:Ho, Anthony Dick |d 910000 |d 910100 |e 910000PH108692477 |e 910100PH108692477 |k 0/910000/ |k 1/910000/910100/ |p 11 |y j | ||
| 998 | |g 123450284 |a Wuchter, Patrick |m 123450284:Wuchter, Patrick |d 910000 |d 910100 |e 910000PW123450284 |e 910100PW123450284 |k 0/910000/ |k 1/910000/910100/ |p 6 | ||
| 998 | |g 143037390 |a Horn, Patrick |m 143037390:Horn, Patrick |d 910000 |d 910100 |e 910000PH143037390 |e 910100PH143037390 |k 0/910000/ |k 1/910000/910100/ |p 4 | ||
| 998 | |g 1060647400 |a Kaufmann, Stefan |m 1060647400:Kaufmann, Stefan |d 120000 |d 120300 |e 120000PK1060647400 |e 120300PK1060647400 |k 0/120000/ |k 1/120000/120300/ |p 2 | ||
| 998 | |g 1151543357 |a Frank, Viktoria |m 1151543357:Frank, Viktoria |d 120000 |d 120300 |e 120000PF1151543357 |e 120300PF1151543357 |k 0/120000/ |k 1/120000/120300/ |p 1 |x j | ||
| 999 | |a KXP-PPN1698678223 |e 3669897089 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"eki":["1698678223"],"doi":["10.1038/srep24264"]},"type":{"bibl":"article-journal","media":"Online-Ressource"},"physDesc":[{"extent":"12 S."}],"note":["Gesehen am 22.05.2020"],"relHost":[{"type":{"media":"Online-Ressource","bibl":"periodical"},"pubHistory":["1, article number 1 (2011)-"],"language":["eng"],"disp":"Frequent mechanical stress suppresses proliferation of mesenchymal stem cells from human bone marrow without loss of multipotencyScientific reports","recId":"663366712","note":["Gesehen am 12.07.24"],"part":{"extent":"12","year":"2016","volume":"6","text":"6(2016) Artikel-Nummer 24264, 12 Seiten"},"id":{"eki":["663366712"],"issn":["2045-2322"],"zdb":["2615211-3"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"Scientific reports","title_sort":"Scientific reports"}],"origin":[{"dateIssuedDisp":"2011-","publisher":"Springer Nature ; Nature Publishing Group","publisherPlace":"[London] ; London","dateIssuedKey":"2011"}]}],"origin":[{"dateIssuedDisp":"15 April 2016","dateIssuedKey":"2016"}],"name":{"displayForm":["Viktoria Frank, Stefan Kaufmann, Rebecca Wright, Patrick Horn, Hiroshi Y. Yoshikawa, Patrick Wuchter, Jeppe Madsen, Andrew L. Lewis, Steven P. Armes, Anthony D. Ho & Motomu Tanaka"]},"recId":"1698678223","person":[{"family":"Frank","display":"Frank, Viktoria","given":"Viktoria","role":"aut"},{"role":"aut","family":"Kaufmann","given":"Stefan","display":"Kaufmann, Stefan"},{"display":"Wright, Rebecca","given":"Rebecca","family":"Wright","role":"aut"},{"role":"aut","display":"Horn, Patrick","given":"Patrick","family":"Horn"},{"given":"Hiroshi Y.","display":"Yoshikawa, Hiroshi Y.","family":"Yoshikawa","role":"aut"},{"role":"aut","display":"Wuchter, Patrick","given":"Patrick","family":"Wuchter"},{"given":"Jeppe","display":"Madsen, Jeppe","family":"Madsen","role":"aut"},{"role":"aut","family":"Lewis","display":"Lewis, Andrew L.","given":"Andrew L."},{"given":"Steven P.","display":"Armes, Steven P.","family":"Armes","role":"aut"},{"role":"aut","given":"Anthony Dick","display":"Ho, Anthony Dick","family":"Ho"},{"given":"Motomu","display":"Tanaka, Motomu","family":"Tanaka","role":"aut"}],"title":[{"title_sort":"Frequent mechanical stress suppresses proliferation of mesenchymal stem cells from human bone marrow without loss of multipotency","title":"Frequent mechanical stress suppresses proliferation of mesenchymal stem cells from human bone marrow without loss of multipotency"}],"language":["eng"]} | ||
| SRT | |a FRANKVIKTOFREQUENTME1520 | ||