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Simple lithography-free single cell micropatterning using laser-cut stencils

Micropatterning techniques have been widely used in cell biology to study effects of controlling cell shape and size on cell fate determination at single cell resolution. Current state-of-the-art single cell micropatterning techniques involve soft lithography and micro-contact printing, which is a p...

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Bibliographic Details
Main Authors: Lee, Soah (Author) , Yang, Huaxiao (Author) , Chen, Caressa (Author) , Venkatraman, Sneha (Author) , Darsha, Adrija (Author) , Wu, Sean M. (Author) , Wu, Joseph C. (Author) , Seeger, Timon (Author)
Format: Article (Journal) Video
Language:English
Published: 03.04.2020
In: JoVE. Video journal
Year: 2020, Issue: 158, Pages: ?
ISSN:1940-087X
DOI:10.3791/60888
Subjects:
Film
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3791/60888
Verlag, lizenzpflichtig, Volltext: https://www.jove.com/video/60888/simple-lithography-free-single-cell-micropatterning-using-laser-cut
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Author Notes:Soah Lee, Huaxiao Yang, Caressa Chen, Sneha Venkatraman, Adrija Darsha, Sean M. Wu, Joseph C. Wu, Timon Seeger
Description
Summary:Micropatterning techniques have been widely used in cell biology to study effects of controlling cell shape and size on cell fate determination at single cell resolution. Current state-of-the-art single cell micropatterning techniques involve soft lithography and micro-contact printing, which is a powerful technology, but requires trained engineering skills and certain facility support in microfabrication. These limitations require a more accessible technique. Here, we describe a simple alternative lithography-free method: stencil-based single cell patterning. We provide step-by-step procedures including stencil design, polyacrylamide hydrogel fabrication, stencil-based protein incorporation, and cell plating and culture. This simple method can be used to pattern an array of as many as 2,000 cells. We demonstrate the patterning of cardiomyocytes derived from single human induced pluripotent stem cells (hiPSC) with distinct cell shapes, from a 1:1 square to a 7:1 adult cardiomyocyte-like rectangle. This stencil-based single cell patterning is lithography-free, technically robust, convenient, inexpensive, and most importantly accessible to those with a limited bioengineering background.
Item Description:Enthält auch Textversion
Gesehen am 03.06.2020
Wissenschaftlicher Film. Deutschland. 2020
Physical Description:Online Resource
ISSN:1940-087X
DOI:10.3791/60888

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