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Association of CD30 transcripts with Th1 responses and proinflammatory cytokines in patients with end-stage renal disease

High serum sCD30 levels are associated with inflammatory disorders and poor outcome in renal transplantation. The contribution to these phenomena of transcripts and proteins related to CD30-activation and -cleavage is unknown. We assessed in peripheral blood of end-stage renal disease patients (ESRD...

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Hauptverfasser: Velásquez, Sonia Y. (VerfasserIn) , Opelz, Gerhard (VerfasserIn) , Rojas, Mauricio (VerfasserIn) , Süsal, Caner (VerfasserIn) , Alvarez, Cristiam M. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 9 March 2016
In: Human immunology
Year: 2016, Jahrgang: 77, Heft: 5, Pages: 403-410
ISSN:1879-1166
DOI:10.1016/j.humimm.2016.03.004
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.humimm.2016.03.004
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0198885916300271
Volltext
Verfasserangaben:Sonia Y. Velásquez, Gerhard Opelz, Mauricio Rojas, Caner Süsal, Cristiam M. Alvarez
Beschreibung
Zusammenfassung:High serum sCD30 levels are associated with inflammatory disorders and poor outcome in renal transplantation. The contribution to these phenomena of transcripts and proteins related to CD30-activation and -cleavage is unknown. We assessed in peripheral blood of end-stage renal disease patients (ESRDP) transcripts of CD30-activation proteins CD30 and CD30L, CD30-cleavage proteins ADAM10 and ADAM17, and Th1- and Th2-type immunity-related factors t-bet and GATA3. Additionally, we evaluated the same transcripts and release of sCD30 and 32 cytokines after allogeneic and polyclonal T-cell activation. In peripheral blood, ESRDP showed increased levels of t-bet and GATA3 transcripts compared to healthy controls (HC) (both P<0.01) whereas levels of CD30, CD30L, ADAM10 and ADAM17 transcripts were similar. Polyclonal and allogeneic stimulation induced higher levels of CD30 transcripts in ESRDP than in HC (both P<0.001). Principal component analysis (PCA) in allogeneic cultures of ESRDP identified two correlation clusters, one consisting of sCD30, the Th-1 cytokine IFN-γ, MIP-1α, RANTES, sIL-2Rα, MIP-1β, TNF-β, MDC, GM-CSF and IL-5, and another one consisting of CD30 and t-bet transcripts, IL-13 and proinflammatory proteins IP-10, IL-8, IL-1Rα and MCP-1. Reflecting an activated immune state, ESRDP exhibited after allostimulation upregulation of CD30 transcripts in T cells, which was associated with Th1 and proinflammatory responses.
Beschreibung:Gesehen am 04.06.2020
Beschreibung:Online Resource
ISSN:1879-1166
DOI:10.1016/j.humimm.2016.03.004

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