Bacterial and Yeast AAA+ Disaggregases ClpB and Hsp104 operate through conserved mechanism involving cooperation with Hsp70
Escherichia coli ClpB and Saccharomyces cerevisiae Hsp104 are members of the Hsp100 family of ring-forming hexameric AAA+ chaperones that promote the solubilization of aggregated proteins and the propagation of prions. ClpB and Hsp104 cooperate with cognate Hsp70 chaperones for substrate targeting a...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
9 September 2016
|
| In: |
Journal of molecular biology
Year: 2016, Volume: 428, Issue: 21, Pages: 4378-4391 |
| ISSN: | 1089-8638 |
| DOI: | 10.1016/j.jmb.2016.09.003 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.jmb.2016.09.003 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0022283616303679 
|
| Author Notes: | Eva Kummer, Anna Szlachcic, Kamila B. Franke, Sophia Ungelenk, Bernd Bukau and Axel Mogk |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1699768447 | ||
| 003 | DE-627 | ||
| 005 | 20220818112043.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200604s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jmb.2016.09.003 |2 doi | |
| 035 | |a (DE-627)1699768447 | ||
| 035 | |a (DE-599)KXP1699768447 | ||
| 035 | |a (OCoLC)1341328420 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Kummer, Eva |e VerfasserIn |0 (DE-588)1027998127 |0 (DE-627)73011161X |0 (DE-576)375474552 |4 aut | |
| 245 | 1 | 0 | |a Bacterial and Yeast AAA+ Disaggregases ClpB and Hsp104 operate through conserved mechanism involving cooperation with Hsp70 |c Eva Kummer, Anna Szlachcic, Kamila B. Franke, Sophia Ungelenk, Bernd Bukau and Axel Mogk |
| 264 | 1 | |c 9 September 2016 | |
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 04.06.2020 | ||
| 520 | |a Escherichia coli ClpB and Saccharomyces cerevisiae Hsp104 are members of the Hsp100 family of ring-forming hexameric AAA+ chaperones that promote the solubilization of aggregated proteins and the propagation of prions. ClpB and Hsp104 cooperate with cognate Hsp70 chaperones for substrate targeting and activation of ATPase and substrate threading, achieved by transient Hsp70 binding to the repressing ClpB/Hsp104 M-domain. Fundamental differences in ATPase regulation and disaggregation mechanisms have been reported; however, these differences are raising doubts regarding the working principle of this AAA+ chaperone. In particular, unique functional plasticity was suggested to specifically enable Hsp104 to circumvent Hsp70 requirement for derepression in protein disaggregation and prion propagation. We show here that both ClpB and Hsp104 cooperation with Hsp70 is crucial for efficient protein disaggregation and, in contrast to earlier claims, cannot be circumvented by activating M-domain mutations. Activation of ClpB and Hsp104 requires two signals, relief of M-domain repression and substrate binding, leading to increased ATPase subunit coupling. These data demonstrate that ClpB and Hsp104 operate by the same basic mechanism, underscore a dominant function of Hsp70 in regulating ClpB/Hsp104 activity, and explain a plethora of in vivo studies showing a crucial function of Hsp70 in proteostasis and prion propagation. | ||
| 650 | 4 | |a AAA+ protein | |
| 650 | 4 | |a chaperone | |
| 650 | 4 | |a heat shock protein | |
| 650 | 4 | |a protein disaggregation | |
| 650 | 4 | |a protein folding | |
| 700 | 1 | |a Szlachcic, Anna |e VerfasserIn |0 (DE-588)107525857X |0 (DE-627)833112724 |0 (DE-576)44334681X |4 aut | |
| 700 | 1 | |a Franke, Kamila B. |e VerfasserIn |0 (DE-588)1136341382 |0 (DE-627)892660058 |0 (DE-576)490449840 |4 aut | |
| 700 | 1 | |a Ungelenk, Sophia Maria |e VerfasserIn |0 (DE-588)1073663981 |0 (DE-627)829319751 |0 (DE-576)435245414 |4 aut | |
| 700 | 1 | |a Bukau, Bernd |d 1954- |e VerfasserIn |0 (DE-588)143209019 |0 (DE-627)643892575 |0 (DE-576)335649270 |4 aut | |
| 700 | 1 | |a Mogk, Axel |e VerfasserIn |0 (DE-588)102799914X |0 (DE-627)73031166X |0 (DE-576)180449338 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of molecular biology |d Amsterdam [u.a.] : Elsevier, 1959 |g 428(2016), 21, Seite 4378-4391 |h Online-Ressource |w (DE-627)222605472 |w (DE-600)1355192-9 |w (DE-576)094059837 |x 1089-8638 |7 nnas |a Bacterial and Yeast AAA+ Disaggregases ClpB and Hsp104 operate through conserved mechanism involving cooperation with Hsp70 |
| 773 | 1 | 8 | |g volume:428 |g year:2016 |g number:21 |g pages:4378-4391 |g extent:14 |a Bacterial and Yeast AAA+ Disaggregases ClpB and Hsp104 operate through conserved mechanism involving cooperation with Hsp70 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.jmb.2016.09.003 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0022283616303679 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200604 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 102799914X |a Mogk, Axel |m 102799914X:Mogk, Axel |d 700000 |d 706000 |e 700000PM102799914X |e 706000PM102799914X |k 0/700000/ |k 1/700000/706000/ |p 6 |y j | ||
| 998 | |g 143209019 |a Bukau, Bernd |m 143209019:Bukau, Bernd |d 700000 |d 706000 |e 700000PB143209019 |e 706000PB143209019 |k 0/700000/ |k 1/700000/706000/ |p 5 | ||
| 998 | |g 1073663981 |a Ungelenk, Sophia Maria |m 1073663981:Ungelenk, Sophia Maria |d 700000 |d 706000 |e 700000PU1073663981 |e 706000PU1073663981 |k 0/700000/ |k 1/700000/706000/ |p 4 | ||
| 998 | |g 1136341382 |a Franke, Kamila B. |m 1136341382:Franke, Kamila B. |d 700000 |d 706000 |e 700000PF1136341382 |e 706000PF1136341382 |k 0/700000/ |k 1/700000/706000/ |p 3 | ||
| 998 | |g 107525857X |a Szlachcic, Anna |m 107525857X:Szlachcic, Anna |d 700000 |d 706000 |e 700000PS107525857X |e 706000PS107525857X |k 0/700000/ |k 1/700000/706000/ |p 2 | ||
| 999 | |a KXP-PPN1699768447 |e 368247790X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"display":"Kummer, Eva","family":"Kummer","role":"aut","given":"Eva"},{"role":"aut","family":"Szlachcic","display":"Szlachcic, Anna","given":"Anna"},{"given":"Kamila B.","display":"Franke, Kamila B.","role":"aut","family":"Franke"},{"given":"Sophia Maria","role":"aut","family":"Ungelenk","display":"Ungelenk, Sophia Maria"},{"given":"Bernd","display":"Bukau, Bernd","family":"Bukau","role":"aut"},{"given":"Axel","display":"Mogk, Axel","family":"Mogk","role":"aut"}],"note":["Gesehen am 04.06.2020"],"language":["eng"],"name":{"displayForm":["Eva Kummer, Anna Szlachcic, Kamila B. Franke, Sophia Ungelenk, Bernd Bukau and Axel Mogk"]},"origin":[{"dateIssuedDisp":"9 September 2016","dateIssuedKey":"2016"}],"recId":"1699768447","id":{"eki":["1699768447"],"doi":["10.1016/j.jmb.2016.09.003"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"physDesc":[{"extent":"14 S."}],"title":[{"title":"Bacterial and Yeast AAA+ Disaggregases ClpB and Hsp104 operate through conserved mechanism involving cooperation with Hsp70","title_sort":"Bacterial and Yeast AAA+ Disaggregases ClpB and Hsp104 operate through conserved mechanism involving cooperation with Hsp70"}],"relHost":[{"type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"222605472","id":{"zdb":["1355192-9"],"eki":["222605472"],"issn":["1089-8638"]},"origin":[{"publisher":"Elsevier ; Academic Press","dateIssuedKey":"1959","publisherPlace":"Amsterdam [u.a.] ; London","dateIssuedDisp":"1959-"}],"disp":"Bacterial and Yeast AAA+ Disaggregases ClpB and Hsp104 operate through conserved mechanism involving cooperation with Hsp70Journal of molecular biology","language":["eng"],"pubHistory":["1.1959 -"],"titleAlt":[{"title":"JMB"}],"note":["Gesehen am 08.03.11"],"part":{"pages":"4378-4391","year":"2016","issue":"21","extent":"14","text":"428(2016), 21, Seite 4378-4391","volume":"428"},"title":[{"subtitle":"JMB","title_sort":"Journal of molecular biology","title":"Journal of molecular biology"}],"physDesc":[{"extent":"Online-Ressource"}]}]} | ||
| SRT | |a KUMMEREVASBACTERIALA9201 | ||