Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actin

Filamentous actin is one of the most important cytoskeletal elements. Not only is it responsible for the elastic properties of many cell types, but it also plays a vital role in cellular adhesion and motility. Understanding the bundling kinetics of actin filaments is important in the formation of va...

Full description

Saved in:
Bibliographic Details
Main Authors: Maier, Timo (Author) , Haraszti, Tamás (Author)
Format: Article (Journal)
Language:English
Published: August 31, 2015
In: PLOS ONE
Year: 2015, Volume: 10, Issue: 8
ISSN:1932-6203
DOI:10.1371/journal.pone.0136432
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0136432
Verlag, lizenzpflichtig, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0136432
Get full text
Author Notes:Timo Maier, Tamás Haraszti

MARC

LEADER 00000caa a2200000 c 4500
001 169995836X
003 DE-627
005 20220818113043.0
007 cr uuu---uuuuu
008 200605s2015 xx |||||o 00| ||eng c
024 7 |a 10.1371/journal.pone.0136432  |2 doi 
035 |a (DE-627)169995836X 
035 |a (DE-599)KXP169995836X 
035 |a (OCoLC)1341328515 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 30  |2 sdnb 
100 1 |a Maier, Timo  |e VerfasserIn  |0 (DE-588)1035816547  |0 (DE-627)749605642  |0 (DE-576)301139032  |4 aut 
245 1 0 |a Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actin  |c Timo Maier, Tamás Haraszti 
264 1 |c August 31, 2015 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 05.06.2020 
520 |a Filamentous actin is one of the most important cytoskeletal elements. Not only is it responsible for the elastic properties of many cell types, but it also plays a vital role in cellular adhesion and motility. Understanding the bundling kinetics of actin filaments is important in the formation of various cytoskeletal structures, such as filopodia and stress fibers. Utilizing a unique pillar-structured microfluidic device, we investigated the time dependence of bundling kinetics of pillar supported free-standing actin filaments. Microparticles attached to the filaments allowed the measurement of thermal motion, and we found that bundling takes place at lower concentrations than previously found in 3-dimensional actin gels, i.e. actin filaments formed bundles in the presence of 5-12 mM of magnesium chloride in a time-dependent manner. The filaments also displayed long term stability for up to hours after removing the magnesium ions from the buffer, which suggests that there is an extensive hysteresis between cation induced crosslinking and decrosslinking. 
650 4 |a Actin filaments 
650 4 |a Actin polymerization 
650 4 |a Cations 
650 4 |a Cross-linking 
650 4 |a Fluorescence imaging 
650 4 |a Ions 
650 4 |a Magnesium 
650 4 |a Polymerization 
700 1 |a Haraszti, Tamás  |e VerfasserIn  |0 (DE-588)1164538764  |0 (DE-627)1028966148  |0 (DE-576)510079342  |4 aut 
773 0 8 |i Enthalten in  |t PLOS ONE  |d San Francisco, California, US : PLOS, 2006  |g 10(2015,8) Artikel-Nummer e0136432, 17 Seiten  |h Online-Ressource  |w (DE-627)523574592  |w (DE-600)2267670-3  |w (DE-576)281331979  |x 1932-6203  |7 nnas  |a Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actin 
773 1 8 |g volume:10  |g year:2015  |g number:8  |a Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actin 
856 4 0 |u https://doi.org/10.1371/journal.pone.0136432  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0136432  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20200605 
993 |a Article 
994 |a 2015 
998 |g 1164538764  |a Haraszti, Tamás  |m 1164538764:Haraszti, Tamás  |p 2  |y j 
998 |g 1035816547  |a Maier, Timo  |m 1035816547:Maier, Timo  |p 1  |x j 
999 |a KXP-PPN169995836X  |e 3683288779 
BIB |a Y 
SER |a journal 
JSO |a {"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 05.06.2020"],"recId":"169995836X","language":["eng"],"relHost":[{"part":{"text":"10(2015,8) Artikel-Nummer e0136432, 17 Seiten","volume":"10","year":"2015","issue":"8"},"pubHistory":["1.2006 -"],"language":["eng"],"corporate":[{"role":"isb","display":"Public Library of Science","roleDisplay":"Herausgebendes Organ"}],"recId":"523574592","disp":"Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actinPLOS ONE","note":["Schreibweise des Titels bis 2012: PLoS ONE","Gesehen am 20.03.19"],"type":{"bibl":"periodical","media":"Online-Ressource"},"title":[{"title":"PLOS ONE","title_sort":"PLOS ONE"}],"physDesc":[{"extent":"Online-Ressource"}],"id":{"issn":["1932-6203"],"eki":["523574592"],"zdb":["2267670-3"]},"origin":[{"publisherPlace":"San Francisco, California, US ; Lawrence, Kan.","dateIssuedDisp":"2006-","dateIssuedKey":"2006","publisher":"PLOS ; PLoS"}],"name":{"displayForm":["Public Library of Science"]}}],"title":[{"title":"Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actin","title_sort":"Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actin"}],"origin":[{"dateIssuedKey":"2015","dateIssuedDisp":"August 31, 2015"}],"id":{"doi":["10.1371/journal.pone.0136432"],"eki":["169995836X"]},"person":[{"roleDisplay":"VerfasserIn","display":"Maier, Timo","role":"aut","family":"Maier","given":"Timo"},{"family":"Haraszti","given":"Tamás","roleDisplay":"VerfasserIn","display":"Haraszti, Tamás","role":"aut"}],"name":{"displayForm":["Timo Maier, Tamás Haraszti"]}} 
SRT |a MAIERTIMOHREVERSIBIL3120