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Multicentre comparison of a diagnostic assay: aquaporin-4 antibodies in neuromyelitis optica

Objective Antibodies to cell surface central nervous system proteins help to diagnose conditions which often respond to immunotherapies. The assessment of antibody assays needs to reflect their clinical utility. We report the results of a multicentre study of aquaporin (AQP) 4 antibody (AQP4-Ab) ass...

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Main Authors: Waterson, Patrick (Author) , Reindl, Markus (Author) , Saiz, Albert (Author) , Schanda, Kathrin (Author) , Tuller, Friederike (Author) , Kral, Vlastimil (Author) , Nytrova, Petra (Author) , Sobek, Ondrej (Author) , Nielsen, Helle Hvilsted (Author) , Barington, Torben (Author) , Lillevang, Søren T. (Author) , Illes, Zsolt (Author) , Rentzsch, Kristin (Author) , Berthele, Achim (Author) , Berki, Tímea (Author) , Granieri, Letizia (Author) , Bertolotto, Antonio (Author) , Giometto, Bruno (Author) , Zuliani, Luigi (Author) , Hamann, Dörte (Author) , Pelt, E. Daniëlle van (Author) , Hintzen, Rogier (Author) , Höftberger, Romana (Author) , Costa, Carme (Author) , Comabella, Manuel (Author) , Montalban, Xavier (Author) , Tintoré, Mar (Author) , Siva, Aksel (Author) , Altintas, Ayse (Author) , Deniz, Günnur (Author) , Woodhall, Mark (Author) , Palace, Jacqueline (Author) , Paul, Friedemann (Author) , Hartung, Hans-Peter (Author) , Aktas, Orhan (Author) , Jarius, Sven (Author) , Wildemann, Brigitte (Author) , Vedeler, Christian (Author) , Ruiz, Anne (Author) , Leite, M. Isabel (Author) , Trillenberg, Peter (Author) , Probst, Monika (Author) , Saschenbrecker, Sandra (Author) , Vincent, Angela (Author) , Marignier, Romain (Author)
Format: Article (Journal)
Language:English
Published: 25 April 2016
In: Journal of neurology, neurosurgery, and psychiatry
Year: 2016, Volume: 87, Issue: 9, Pages: 1005-1015
ISSN:1468-330X
DOI:10.1136/jnnp-2015-312601
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1136/jnnp-2015-312601
Verlag, lizenzpflichtig, Volltext: https://jnnp.bmj.com/content/87/9/1005
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Author Notes:Patrick Waters, Markus Reindl, Albert Saiz, Kathrin Schanda, Friederike Tuller, Vlastimil Kral, Petra Nytrova, Ondrej Sobek, Helle Hvilsted Nielsen, Torben Barington, Søren T. Lillevang, Zsolt Illes, Kristin Rentzsch, Achim Berthele, Tímea Berki, Letizia Granieri, Antonio Bertolotto, Bruno Giometto, Luigi Zuliani, Dörte Hamann, E. Daniëlle van Pelt, Rogier Hintzen, Romana Höftberger, Carme Costa, Manuel Comabella, Xavier Montalban, Mar Tintoré, Aksel Siva, Ayse Altintas, Günnur Deniz, Mark Woodhall, Jacqueline Palace, Friedemann Paul, Hans-Peter Hartung, Orhan Aktas, Sven Jarius, Brigitte Wildemann, Christian Vedeler, Anne Ruiz, M. Isabel Leite, Peter Trillenberg, Monika Probst, Sandra Saschenbrecker, Angela Vincent, Romain Marignier

MARC

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245 1 0 |a Multicentre comparison of a diagnostic assay  |b aquaporin-4 antibodies in neuromyelitis optica  |c Patrick Waters, Markus Reindl, Albert Saiz, Kathrin Schanda, Friederike Tuller, Vlastimil Kral, Petra Nytrova, Ondrej Sobek, Helle Hvilsted Nielsen, Torben Barington, Søren T. Lillevang, Zsolt Illes, Kristin Rentzsch, Achim Berthele, Tímea Berki, Letizia Granieri, Antonio Bertolotto, Bruno Giometto, Luigi Zuliani, Dörte Hamann, E. Daniëlle van Pelt, Rogier Hintzen, Romana Höftberger, Carme Costa, Manuel Comabella, Xavier Montalban, Mar Tintoré, Aksel Siva, Ayse Altintas, Günnur Deniz, Mark Woodhall, Jacqueline Palace, Friedemann Paul, Hans-Peter Hartung, Orhan Aktas, Sven Jarius, Brigitte Wildemann, Christian Vedeler, Anne Ruiz, M. Isabel Leite, Peter Trillenberg, Monika Probst, Sandra Saschenbrecker, Angela Vincent, Romain Marignier 
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520 |a Objective Antibodies to cell surface central nervous system proteins help to diagnose conditions which often respond to immunotherapies. The assessment of antibody assays needs to reflect their clinical utility. We report the results of a multicentre study of aquaporin (AQP) 4 antibody (AQP4-Ab) assays in neuromyelitis optica spectrum disorders (NMOSD). - Methods Coded samples from patients with neuromyelitis optica (NMO) or NMOSD (101) and controls (92) were tested at 15 European diagnostic centres using 21 assays including live (n=3) or fixed cell-based assays (n=10), flow cytometry (n=4), immunohistochemistry (n=3) and ELISA (n=1). - Results Results of tests on 92 controls identified 12assays as highly specific (0-1 false-positive results). 32 samples from 50 (64%) NMO sera and 34 from 51 (67%) NMOSD sera were positive on at least two of the 12 highly specific assays, leaving 35 patients with seronegative NMO/spectrum disorder (SD). On the basis of a combination of clinical phenotype and the highly specific assays, 66 AQP4-Ab seropositive samples were used to establish the sensitivities (51.5-100%) of all 21 assays. The specificities (85.8-100%) were based on 92 control samples and 35 seronegative NMO/SD patient samples. - Conclusions The cell-based assays were most sensitive and specific overall, but immunohistochemistry or flow cytometry could be equally accurate in specialist centres. Since patients with AQP4-Ab negative NMO/SD require different management, the use of both appropriate control samples and defined seronegative NMOSD samples is essential to evaluate these assays in a clinically meaningful way. The process described here can be applied to the evaluation of other antibody assays in the newly evolving field of autoimmune neurology. 
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