Cover Image

Post-conversion sialylation of prions in lymphoid tissues

Sialylated glycans on the surface of mammalian cells act as part of a “self-associated molecular pattern,” helping the immune system to recognize “self” from “altered self” or “nonself.” To escape the host immune system, some bacterial pathogens have evolved biosynthetic pathways for host-like siali...

Full description

Saved in:
Bibliographic Details
Main Authors: Srivastava, Saurabh (Author) , Makarava, Natallia (Author) , Katorcha, Elizaveta (Author) , Savtchenko, Regina (Author) , Brossmer, Reinhard (Author) , Baskakov, Ilia V. (Author)
Format: Article (Journal)
Language:English
Published: November 16, 2015
In: Proceedings of the National Academy of Sciences of the United States of America
Year: 2015, Volume: 112, Issue: 48, Pages: E6654-E6662
ISSN:1091-6490
DOI:10.1073/pnas.1517993112
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1073/pnas.1517993112
Verlag, lizenzpflichtig, Volltext: https://www.pnas.org/content/112/48/E6654
Get full text
Author Notes:Saurabh Srivastava, Natallia Makarava, Elizaveta Katorcha, Regina Savtchenko, Reinhard Brossmer, and Ilia V. Baskakov

MARC

LEADER 00000caa a2200000 c 4500
001 1701701677
003 DE-627
005 20220818130004.0
007 cr uuu---uuuuu
008 200622s2015 xx |||||o 00| ||eng c
024 7 |a 10.1073/pnas.1517993112  |2 doi 
035 |a (DE-627)1701701677 
035 |a (DE-599)KXP1701701677 
035 |a (OCoLC)1341340863 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Srivastava, Saurabh  |e VerfasserIn  |0 (DE-588)1212378741  |0 (DE-627)1701702355  |4 aut 
245 1 0 |a Post-conversion sialylation of prions in lymphoid tissues  |c Saurabh Srivastava, Natallia Makarava, Elizaveta Katorcha, Regina Savtchenko, Reinhard Brossmer, and Ilia V. Baskakov 
264 1 |c November 16, 2015 
300 |a 9 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 22.06.2020 
520 |a Sialylated glycans on the surface of mammalian cells act as part of a “self-associated molecular pattern,” helping the immune system to recognize “self” from “altered self” or “nonself.” To escape the host immune system, some bacterial pathogens have evolved biosynthetic pathways for host-like sialic acids, whereas others recruited host sialic acids for decorating their surfaces. Prions lack nucleic acids and are not conventional pathogens. Nevertheless, prions might use a similar strategy for invading and colonizing the lymphoreticular system. Here we show that the sialylation status of the infectious, disease-associated state of the prion protein (PrPSc) changes with colonization of secondary lymphoid organs (SLOs). As a result, spleen-derived PrPSc is more sialylated than brain-derived PrPSc. Enhanced sialylation of PrPSc is recapitulated in vitro by incubating brain-derived PrPSc with primary splenocytes or cultured macrophage RAW 264.7 cells. General inhibitors of sialyltranserases (STs), the enzymes that transfer sialic acid residues onto terminal positions of glycans, suppressed extrasialylation of PrPSc. A fluorescently labeled precursor of sialic acid revealed ST activity associated with RAW macrophages. This study illustrates that, upon colonization of SLOs, the sialylation status of prions changes by host STs. We propose that this mechanism is responsible for camouflaging prions in SLOs and has broad implications. 
650 4 |a macrophages 
650 4 |a prion diseases 
650 4 |a prions 
650 4 |a sialic acid 
650 4 |a sialylated glycans 
700 1 |a Makarava, Natallia  |e VerfasserIn  |4 aut 
700 1 |a Katorcha, Elizaveta  |e VerfasserIn  |4 aut 
700 1 |a Savtchenko, Regina  |e VerfasserIn  |4 aut 
700 1 |a Brossmer, Reinhard  |d 1925-2020  |e VerfasserIn  |0 (DE-588)1115343556  |0 (DE-627)869722298  |0 (DE-576)324407076  |4 aut 
700 1 |a Baskakov, Ilia V.  |e VerfasserIn  |4 aut 
773 0 8 |i Enthalten in  |a National Academy of Sciences (Washington, DC)  |t Proceedings of the National Academy of Sciences of the United States of America  |d Washington, DC : National Acad. of Sciences, 1915  |g 112(2015), 48, Seite E6654-E6662  |h Online-Ressource  |w (DE-627)254235379  |w (DE-600)1461794-8  |w (DE-576)073260509  |x 1091-6490  |7 nnas 
773 1 8 |g volume:112  |g year:2015  |g number:48  |g pages:E6654-E6662  |g extent:9  |a Post-conversion sialylation of prions in lymphoid tissues 
856 4 0 |u https://doi.org/10.1073/pnas.1517993112  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.pnas.org/content/112/48/E6654  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20200622 
993 |a Article 
994 |a 2015 
998 |g 1115343556  |a Brossmer, Reinhard  |m 1115343556:Brossmer, Reinhard  |d 140000  |e 140000PB1115343556  |k 0/140000/  |p 5 
999 |a KXP-PPN1701701677  |e 369021288X 
BIB |a Y 
SER |a journal 
JSO |a {"person":[{"family":"Srivastava","given":"Saurabh","display":"Srivastava, Saurabh","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Makarava","given":"Natallia","roleDisplay":"VerfasserIn","display":"Makarava, Natallia","role":"aut"},{"display":"Katorcha, Elizaveta","roleDisplay":"VerfasserIn","role":"aut","family":"Katorcha","given":"Elizaveta"},{"family":"Savtchenko","given":"Regina","roleDisplay":"VerfasserIn","display":"Savtchenko, Regina","role":"aut"},{"given":"Reinhard","family":"Brossmer","role":"aut","roleDisplay":"VerfasserIn","display":"Brossmer, Reinhard"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Baskakov, Ilia V.","given":"Ilia V.","family":"Baskakov"}],"title":[{"title_sort":"Post-conversion sialylation of prions in lymphoid tissues","title":"Post-conversion sialylation of prions in lymphoid tissues"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 22.06.2020"],"recId":"1701701677","language":["eng"],"name":{"displayForm":["Saurabh Srivastava, Natallia Makarava, Elizaveta Katorcha, Regina Savtchenko, Reinhard Brossmer, and Ilia V. Baskakov"]},"origin":[{"dateIssuedKey":"2015","dateIssuedDisp":"November 16, 2015"}],"id":{"eki":["1701701677"],"doi":["10.1073/pnas.1517993112"]},"physDesc":[{"extent":"9 S."}],"relHost":[{"id":{"zdb":["1461794-8"],"eki":["254235379"],"issn":["1091-6490"]},"origin":[{"publisherPlace":"Washington, DC","dateIssuedKey":"1915","publisher":"National Acad. of Sciences","dateIssuedDisp":"1915-"}],"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Proceedings of the National Academy of Sciences of the United States of America","title":"Proceedings of the National Academy of Sciences of the United States of America"}],"recId":"254235379","language":["eng"],"corporate":[{"role":"aut","roleDisplay":"VerfasserIn","display":"National Academy of Sciences"}],"disp":"National Academy of Sciences (Washington, DC)Proceedings of the National Academy of Sciences of the United States of America","type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 09.03.2022"],"titleAlt":[{"title":"PNAS online"},{"title":"PNAS"}],"part":{"issue":"48","pages":"E6654-E6662","year":"2015","extent":"9","text":"112(2015), 48, Seite E6654-E6662","volume":"112"},"pubHistory":["1.1915 -"]}]} 
SRT |a SRIVASTAVAPOSTCONVER1620 

Similar Items