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Regional cell-specific transcriptome mapping reveals regulatory complexity in the adult Drosophila midgut

Deciphering contributions of specific cell types to organ function is experimentally challenging. The Drosophila midgut is a dynamic organ with five morphologically and functionally distinct regions (R1-R5), each composed of multipotent intestinal stem cells (ISCs), progenitor enteroblasts (EBs), en...

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Hauptverfasser: Dutta, Devanjali (VerfasserIn) , Dobson, Adam J. (VerfasserIn) , Houtz, Philip L. (VerfasserIn) , Gläßer, Christine (VerfasserIn) , Revah, Jonathan (VerfasserIn) , Korzelius, Jerome (VerfasserIn) , Patel, Parthive H. (VerfasserIn) , Edgar, Bruce (VerfasserIn) , Buchon, Nicolas (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2 July 2015
In: Cell reports
Year: 2015, Jahrgang: 12, Heft: 2, Pages: 346-358
ISSN:2211-1247
DOI:10.1016/j.celrep.2015.06.009
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.celrep.2015.06.009
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S2211124715006075
Volltext
Verfasserangaben:Devanjali Dutta, Adam J. Dobson, Philip L. Houtz, Christine Gläßer, Jonathan Revah, Jerome Korzelius, Parthive H. Patel, Bruce A. Edgar, and Nicolas Buchon
Beschreibung
Zusammenfassung:Deciphering contributions of specific cell types to organ function is experimentally challenging. The Drosophila midgut is a dynamic organ with five morphologically and functionally distinct regions (R1-R5), each composed of multipotent intestinal stem cells (ISCs), progenitor enteroblasts (EBs), enteroendocrine cells (EEs), enterocytes (ECs), and visceral muscle (VM). To characterize cellular specialization and regional function in this organ, we generated RNA-sequencing transcriptomes of all five cell types isolated by FACS from each of the five regions, R1-R5. In doing so, we identify transcriptional diversities among cell types and document regional differences within each cell type that define further specialization. We validate cell-specific and regional Gal4 drivers; demonstrate roles for transporter Smvt and transcription factors GATAe, Sna, and Ptx1 in global and regional ISC regulation, and study the transcriptional response of midgut cells upon infection. The resulting transcriptome database (http://flygutseq.buchonlab.com) will foster studies of regionalization, homeostasis, immunity, and cell-cell interactions.
Beschreibung:Gesehen am 02.07.2020
Beschreibung:Online Resource
ISSN:2211-1247
DOI:10.1016/j.celrep.2015.06.009

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