When the label matters: adsorption of labeled and unlabeled proteins on charged surfaces
Fluorescent labels are often attached to proteins to monitor binding and adsorption processes. Docking simulations for native hen egg white lysozyme (HEWL) and HEWL labeled with fluorescein isothiocyanate show that these adsorb differently on charged surfaces. Attachment of even a small label can si...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
October 22, 2015
|
| In: |
Nano letters
Year: 2015, Jahrgang: 15, Heft: 11, Pages: 7508-7513 |
| ISSN: | 1530-6992 |
| DOI: | 10.1021/acs.nanolett.5b03168 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/acs.nanolett.5b03168
|
| Verfasserangaben: | Julia Romanowska, Daria B. Kokh, Rebecca C. Wade |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1703317394 | ||
| 003 | DE-627 | ||
| 005 | 20220818141217.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200702s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1021/acs.nanolett.5b03168 |2 doi | |
| 035 | |a (DE-627)1703317394 | ||
| 035 | |a (DE-599)KXP1703317394 | ||
| 035 | |a (OCoLC)1341344443 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Romanowska, Julia |e VerfasserIn |0 (DE-588)1213059011 |0 (DE-627)1703318137 |4 aut | |
| 245 | 1 | 0 | |a When the label matters |b adsorption of labeled and unlabeled proteins on charged surfaces |c Julia Romanowska, Daria B. Kokh, Rebecca C. Wade |
| 264 | 1 | |c October 22, 2015 | |
| 300 | |b Illustrationen | ||
| 300 | |a 6 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 02.07.2020 | ||
| 520 | |a Fluorescent labels are often attached to proteins to monitor binding and adsorption processes. Docking simulations for native hen egg white lysozyme (HEWL) and HEWL labeled with fluorescein isothiocyanate show that these adsorb differently on charged surfaces. Attachment of even a small label can significantly change the interaction properties of a protein. Thus, the results of experiments with fluorescently labeled proteins should be interpreted by modeling the structures and computing the interaction properties of both labeled and unlabeled species. | ||
| 700 | 1 | |a Kokh, Daria B. |e VerfasserIn |0 (DE-588)1150707844 |0 (DE-627)1010913387 |0 (DE-576)497188945 |4 aut | |
| 700 | 1 | |a Wade, Rebecca C. |e VerfasserIn |0 (DE-588)102801774X |0 (DE-627)730136000 |0 (DE-576)276591402 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nano letters |d Washington, DC : ACS Publ., 2001 |g 15(2015), 11, Seite 7508-7513 |h Online-Ressource |w (DE-627)330078771 |w (DE-600)2048866-X |w (DE-576)094479208 |x 1530-6992 |7 nnas |a When the label matters adsorption of labeled and unlabeled proteins on charged surfaces |
| 773 | 1 | 8 | |g volume:15 |g year:2015 |g number:11 |g pages:7508-7513 |g extent:6 |a When the label matters adsorption of labeled and unlabeled proteins on charged surfaces |
| 856 | 4 | 0 | |u https://doi.org/10.1021/acs.nanolett.5b03168 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200702 | ||
| 993 | |a Article | ||
| 994 | |a 2015 | ||
| 998 | |g 102801774X |a Wade, Rebecca C. |m 102801774X:Wade, Rebecca C. |d 140000 |e 140000PW102801774X |k 0/140000/ |p 3 |y j | ||
| 998 | |g 1150707844 |a Kokh, Daria B. |m 1150707844:Kokh, Daria B. |p 2 | ||
| 999 | |a KXP-PPN1703317394 |e 3694726373 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 02.07.2020"],"relHost":[{"pubHistory":["1.2001 -"],"note":["Gesehen am 22.03.23"],"titleAlt":[{"title":"Nanoletters"}],"part":{"issue":"11","year":"2015","text":"15(2015), 11, Seite 7508-7513","extent":"6","volume":"15","pages":"7508-7513"},"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"corporate":[{"display":"American Chemical Society","role":"isb"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"title":[{"title":"Nano letters","subtitle":"a journal dedicated to nanoscience and nanotechnology","title_sort":"Nano letters"}],"disp":"When the label matters adsorption of labeled and unlabeled proteins on charged surfacesNano letters","recId":"330078771","name":{"displayForm":["publ. by the American Chemical Society"]},"origin":[{"publisher":"ACS Publ.","dateIssuedKey":"2001","dateIssuedDisp":"2001-","publisherPlace":"Washington, DC"}],"id":{"eki":["330078771"],"issn":["1530-6992"],"zdb":["2048866-X"]}}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"physDesc":[{"extent":"6 S.","noteIll":"Illustrationen"}],"title":[{"title":"When the label matters","subtitle":"adsorption of labeled and unlabeled proteins on charged surfaces","title_sort":"When the label matters"}],"person":[{"family":"Romanowska","role":"aut","given":"Julia","display":"Romanowska, Julia"},{"family":"Kokh","given":"Daria B.","role":"aut","display":"Kokh, Daria B."},{"display":"Wade, Rebecca C.","role":"aut","given":"Rebecca C.","family":"Wade"}],"recId":"1703317394","name":{"displayForm":["Julia Romanowska, Daria B. Kokh, Rebecca C. Wade"]},"origin":[{"dateIssuedKey":"2015","dateIssuedDisp":"October 22, 2015"}],"id":{"doi":["10.1021/acs.nanolett.5b03168"],"eki":["1703317394"]}} | ||
| SRT | |a ROMANOWSKAWHENTHELAB2220 | ||