Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors
The causal interrogation of neuronal networks involved in specific behaviors requires the spatially and temporally controlled modulation of neuronal activity. For long-term manipulation of neuronal activity, chemogenetic tools provide a reasonable alternative to short-term optogenetic approaches. He...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
30 August 2016
|
| In: |
Frontiers in molecular neuroscience
Year: 2016, Volume: 9, Pages: 75 |
| ISSN: | 1662-5099 |
| DOI: | 10.3389/fnmol.2016.00075 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3389/fnmol.2016.00075 Verlag, lizenzpflichtig, Volltext: https://www.frontiersin.org/articles/10.3389/fnmol.2016.00075/full 
|
| Author Notes: | Horst A. Obenhaus, Andrei Rozov, Ilaria Bertocchi, Wannan Tang, Joachim Kirsch, Heinrich Betz and Rolf Sprengel |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1703422015 | ||
| 003 | DE-627 | ||
| 005 | 20220818141631.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200703s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3389/fnmol.2016.00075 |2 doi | |
| 035 | |a (DE-627)1703422015 | ||
| 035 | |a (DE-599)KXP1703422015 | ||
| 035 | |a (OCoLC)1341344480 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Obenhaus, Horst-Andreas |e VerfasserIn |0 (DE-588)1124550356 |0 (DE-627)878737871 |0 (DE-576)482820853 |4 aut | |
| 245 | 1 | 0 | |a Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors |c Horst A. Obenhaus, Andrei Rozov, Ilaria Bertocchi, Wannan Tang, Joachim Kirsch, Heinrich Betz and Rolf Sprengel |
| 264 | 1 | |c 30 August 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 03.07.2020 | ||
| 520 | |a The causal interrogation of neuronal networks involved in specific behaviors requires the spatially and temporally controlled modulation of neuronal activity. For long-term manipulation of neuronal activity, chemogenetic tools provide a reasonable alternative to short-term optogenetic approaches. Here we show that virus mediated gene transfer of the ivermectin (IVM) activated glycine receptor mutant GlyRα1AG can be used for the selective and reversible silencing of specific neuronal networks in mice. In the striatum, dorsal hippocampus and olfactory bulb (OB), GlyRα1AG promoted IVM dependent effects in representative behavioral assays. Moreover, GlyRα1AG mediated silencing had a strong and reversible impact on neuronal ensemble activity and c-Fos activation in the OB. Together our results demonstrate that long-term, reversible and re-inducible neuronal silencing via GlyRα1AG is a promising tool for the interrogation of network mechanisms underlying the control of behavior and memory formation. | ||
| 650 | 4 | |a glycine receptor | |
| 650 | 4 | |a Ivermectin | |
| 650 | 4 | |a Neuronal silencing | |
| 650 | 4 | |a odor discrimination | |
| 650 | 4 | |a rAAV | |
| 700 | 1 | |a Rozov, Andrei |d 1968- |e VerfasserIn |0 (DE-588)122769686 |0 (DE-627)706036190 |0 (DE-576)293413312 |4 aut | |
| 700 | 1 | |a Bertocchi, Ilaria |e VerfasserIn |4 aut | |
| 700 | 1 | |a Tang, Wannan |e VerfasserIn |0 (DE-588)138549842 |0 (DE-627)60380229X |0 (DE-576)308011198 |4 aut | |
| 700 | 1 | |a Kirsch, Joachim |d 1958- |e VerfasserIn |0 (DE-588)103280825X |0 (DE-627)739347020 |0 (DE-576)180113879 |4 aut | |
| 700 | 1 | |a Betz, Heinrich |d 1944- |e VerfasserIn |0 (DE-588)143082213 |0 (DE-627)70438373X |0 (DE-576)33493091X |4 aut | |
| 700 | 1 | |a Sprengel, Rolf |d 1955- |e VerfasserIn |0 (DE-588)1060395665 |0 (DE-627)799661228 |0 (DE-576)163694230 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Frontiers in molecular neuroscience |d Lausanne : Frontiers Research Foundation, 2008 |g 9(2016) Artikel-Nummer 75, 17 Seiten |h Online-Ressource |w (DE-627)579826449 |w (DE-600)2452967-9 |w (DE-576)286502267 |x 1662-5099 |7 nnas |a Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors |
| 773 | 1 | 8 | |g volume:9 |g year:2016 |g pages:75 |a Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors |
| 856 | 4 | 0 | |u https://doi.org/10.3389/fnmol.2016.00075 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.frontiersin.org/articles/10.3389/fnmol.2016.00075/full |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200703 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 1124550356 |a Obenhaus, Horst-Andreas |m 1124550356:Obenhaus, Horst-Andreas |p 1 |x j | ||
| 998 | |g 1060395665 |a Sprengel, Rolf |m 1060395665:Sprengel, Rolf |p 7 |y j | ||
| 998 | |g 143082213 |a Betz, Heinrich |m 143082213:Betz, Heinrich |p 6 | ||
| 998 | |g 103280825X |a Kirsch, Joachim |m 103280825X:Kirsch, Joachim |d 50000 |d 53100 |e 50000PK103280825X |e 53100PK103280825X |k 0/50000/ |k 1/50000/53100/ |p 5 | ||
| 998 | |g 138549842 |a Tang, Wannan |m 138549842:Tang, Wannan |p 4 | ||
| 998 | |g 122769686 |a Rozov, Andrei |m 122769686:Rozov, Andrei |p 2 | ||
| 999 | |a KXP-PPN1703422015 |e 3695194103 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 03.07.2020"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"origin":[{"dateIssuedKey":"2016","dateIssuedDisp":"30 August 2016"}],"title":[{"title":"Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors","title_sort":"Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors"}],"language":["eng"],"person":[{"role":"aut","given":"Horst-Andreas","display":"Obenhaus, Horst-Andreas","family":"Obenhaus"},{"given":"Andrei","role":"aut","display":"Rozov, Andrei","family":"Rozov"},{"role":"aut","given":"Ilaria","display":"Bertocchi, Ilaria","family":"Bertocchi"},{"family":"Tang","display":"Tang, Wannan","role":"aut","given":"Wannan"},{"family":"Kirsch","display":"Kirsch, Joachim","role":"aut","given":"Joachim"},{"display":"Betz, Heinrich","family":"Betz","given":"Heinrich","role":"aut"},{"given":"Rolf","role":"aut","family":"Sprengel","display":"Sprengel, Rolf"}],"relHost":[{"id":{"zdb":["2452967-9"],"eki":["579826449"],"issn":["1662-5099"]},"recId":"579826449","pubHistory":["2008 -"],"physDesc":[{"extent":"Online-Ressource"}],"name":{"displayForm":["Frontiers Research Foundation"]},"language":["eng"],"part":{"volume":"9","pages":"75","text":"9(2016) Artikel-Nummer 75, 17 Seiten","year":"2016"},"note":["Gesehen am 04.06.20"],"origin":[{"dateIssuedKey":"2008","publisher":"Frontiers Research Foundation","dateIssuedDisp":"2008-","publisherPlace":"Lausanne"}],"title":[{"title_sort":"Frontiers in molecular neuroscience","title":"Frontiers in molecular neuroscience"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptorsFrontiers in molecular neuroscience"}],"id":{"eki":["1703422015"],"doi":["10.3389/fnmol.2016.00075"]},"name":{"displayForm":["Horst A. Obenhaus, Andrei Rozov, Ilaria Bertocchi, Wannan Tang, Joachim Kirsch, Heinrich Betz and Rolf Sprengel"]},"recId":"1703422015"} | ||
| SRT | |a OBENHAUSHOCAUSALINTE3020 | ||