454-sequencingTM for the KEL, JR, and LAN blood groups
The KEL (ISBT 006), JR (ISBT 032), and LAN (ISBT 033) blood group systems are defined by a complex genetics with a large number of exons, and numerous gene variants. In order to sequence the 53 coding exons and flanking intron regions of all three blood group genes we developed a next-generation-seq...
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| Main Authors: | , |
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| Format: | Chapter/Article |
| Language: | English |
| Published: |
2015
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| In: |
Molecular typing of blood cell antigens
Year: 2015, Pages: 123-133 |
| DOI: | 10.1007/978-1-4939-2690-9_11 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/978-1-4939-2690-9_11
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| Author Notes: | Carola Wieckhusen, Peter Bugert |
| Summary: | The KEL (ISBT 006), JR (ISBT 032), and LAN (ISBT 033) blood group systems are defined by a complex genetics with a large number of exons, and numerous gene variants. In order to sequence the 53 coding exons and flanking intron regions of all three blood group genes we developed a next-generation-sequencing method using 454-sequencing™ on a Genome Sequencer (GS) Junior™ system. Multiplex analysis of eight individual DNA samples was achieved using molecular identifiers. |
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| Item Description: | Im Titel ist TM hochgestellt Gesehen am 06.07.2020 |
| Physical Description: | Online Resource |
| ISBN: | 9781493926909 |
| DOI: | 10.1007/978-1-4939-2690-9_11 |