Cover Image

Contractility measurements on isolated papillary muscles for the investigation of cardiac inotropy in mice

Papillary muscle isolated from adult mouse hearts can be used to study cardiac contractility during different physiological/pathological conditions. The contractile characteristics can be evaluated independently of external influences such as vascular tonus or neurohumoral status. It depicts a scien...

Full description

Saved in:
Bibliographic Details
Main Authors: Uhl, Sebastian (Author) , Freichel, Marc (Author) , Mathar, Ilka (Author)
Format: Article (Journal) Video
Language:English
Published: September 17, 2015
In: JoVE. Video journal
Year: 2015, Issue: 103
ISSN:1940-087X
DOI:10.3791/53076
Subjects:
Film
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3791/53076
Verlag, lizenzpflichtig, Volltext: https://www.jove.com/v/53076/contractility-measurements-on-isolated-papillary-muscles-for
Get full text
Author Notes:Sebastian Uhl, Marc Freichel, Ilka Mathar

MARC

LEADER 00000cga a2200000 c 4500
001 1703804570
003 DE-627
005 20230621152000.0
006 m o | |
007 cr uuu---uuuuu
008 200707s2015 xx ||| | o ||eng c
024 7 |a 10.3791/53076  |2 doi 
035 |a (DE-627)1703804570 
035 |a (DE-599)KXP1703804570 
035 |a (OCoLC)1341344757 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Uhl, Sebastian  |e VerfasserIn  |0 (DE-588)1213296994  |0 (DE-627)1703804384  |4 aut 
245 1 0 |a Contractility measurements on isolated papillary muscles for the investigation of cardiac inotropy in mice  |c Sebastian Uhl, Marc Freichel, Ilka Mathar 
264 1 |c September 17, 2015 
300 |a 1 Online-Ressource (1 Videodatei, 6:22 min)  |b farbig 
300 |a 10 
336 |a Text  |b txt  |2 rdacontent 
336 |a zweidimensionales bewegtes Bild  |b tdi  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 07.07.2020 
500 |a Enthält auch Textversion 
500 |a Wissenschaftlicher Film. Deutschland. 2015 
520 |a Papillary muscle isolated from adult mouse hearts can be used to study cardiac contractility during different physiological/pathological conditions. The contractile characteristics can be evaluated independently of external influences such as vascular tonus or neurohumoral status. It depicts a scientific approach between single cell measurements with isolated cardiac myocytes and in vivo studies like echocardiography. Thus, papillary muscle preparations serve as an excellent model to study cardiac physiology/pathophysiology and can be used for investigations like the modulation by pharmacological agents or the exploration of transgenic animal models. Here, we describe a method of isolating the murine left anterior papillary muscle to investigate cardiac contractility in an organ bath setup. In contrast to a muscle strip preparation isolated from the ventricular wall, the papillary muscle can be prepared in toto without damaging the muscle tissue severely. The organ bath setup consists of several temperature-controlled, gassed and electrode-equipped organ bath chambers. The isolated papillary muscle is fixed in the organ bath chamber and electrically stimulated. The evoked twitch force is recorded using a pressure transducer and parameters such as twitch force amplitude and twitch kinetics are analyzed. Different experimental protocols can be performed to investigate the calcium- and frequency-dependent contractility as well as dose-response curves of contractile agents such as catecholamines or other pharmaceuticals. Additionally, pathologic conditions like acute ischemia can be simulated. 
655 7 |a Film  |0 (DE-588)4017102-4  |0 (DE-627)104559683  |0 (DE-576)208918531  |2 gnd-content 
700 1 |a Freichel, Marc  |d 1968-  |e VerfasserIn  |0 (DE-588)17286819X  |0 (DE-627)697797147  |0 (DE-576)133723615  |4 aut 
700 1 |a Mathar, Ilka  |d 1981-  |e VerfasserIn  |0 (DE-588)1011764326  |0 (DE-627)704803275  |0 (DE-576)339316675  |4 aut 
773 0 8 |i Enthalten in  |t JoVE. Video journal  |d [Erscheinungsort nicht ermittelbar] : [Verlag nicht ermittelbar], 2006  |g (2015,103) Artikel-Nummer e53076, 10 Seiten  |h Online-Ressource  |w (DE-627)521454778  |w (DE-600)2259946-0  |w (DE-576)338522980  |x 1940-087X  |7 nngs  |a Contractility measurements on isolated papillary muscles for the investigation of cardiac inotropy in mice 
773 1 8 |g year:2015  |g number:103  |g extent:10  |a Contractility measurements on isolated papillary muscles for the investigation of cardiac inotropy in mice 
856 4 0 |u https://doi.org/10.3791/53076  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.jove.com/v/53076/contractility-measurements-on-isolated-papillary-muscles-for  |x Verlag  |z lizenzpflichtig  |3 Volltext 
935 |c vide 
937 |a Wissenschaftlicher Film  |b Deutschland  |c 2015 
951 |a AR 
992 |a 20200707 
993 |a Video 
994 |a 2015 
998 |g 1011764326  |a Mathar, Ilka  |m 1011764326:Mathar, Ilka  |d 50000  |d 54600  |e 50000PM1011764326  |e 54600PM1011764326  |k 0/50000/  |k 1/50000/54600/  |p 3 
998 |g 17286819X  |a Freichel, Marc  |m 17286819X:Freichel, Marc  |d 50000  |d 54600  |e 50000PF17286819X  |e 54600PF17286819X  |k 0/50000/  |k 1/50000/54600/  |p 2 
998 |g 1213296994  |a Uhl, Sebastian  |m 1213296994:Uhl, Sebastian  |d 910000  |d 910500  |e 910000PU1213296994  |e 910500PU1213296994  |k 0/910000/  |k 1/910000/910500/  |p 1  |x j  |y j 
999 |a KXP-PPN1703804570  |e 3696013633 
BIB |a Y 
SER |a journal 
JSO |a {"person":[{"family":"Uhl","role":"aut","display":"Uhl, Sebastian","given":"Sebastian"},{"display":"Freichel, Marc","role":"aut","family":"Freichel","given":"Marc"},{"family":"Mathar","role":"aut","display":"Mathar, Ilka","given":"Ilka"}],"title":[{"title":"Contractility measurements on isolated papillary muscles for the investigation of cardiac inotropy in mice","title_sort":"Contractility measurements on isolated papillary muscles for the investigation of cardiac inotropy in mice"}],"origin":[{"dateIssuedDisp":"September 17, 2015","dateIssuedKey":"2015"}],"name":{"displayForm":["Sebastian Uhl, Marc Freichel, Ilka Mathar"]},"id":{"doi":["10.3791/53076"],"eki":["1703804570"]},"recId":"1703804570","physDesc":[{"noteIll":"farbig","extent":"10 S."}],"language":["eng"],"note":["Gesehen am 07.07.2020","Enthält auch Textversion","Wissenschaftlicher Film. Deutschland. 2015"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"relHost":[{"id":{"eki":["521454778"],"zdb":["2259946-0"],"issn":["1940-087X"]},"disp":"Contractility measurements on isolated papillary muscles for the investigation of cardiac inotropy in miceJoVE. Video journal","physDesc":[{"extent":"Online-Ressource"}],"part":{"issue":"103","text":"(2015,103) Artikel-Nummer e53076, 10 Seiten","year":"2015","extent":"10"},"language":["eng"],"recId":"521454778","title":[{"title_sort":"JoVE","partname":"Video journal","title":"JoVE"}],"origin":[{"publisherPlace":"[Erscheinungsort nicht ermittelbar]","dateIssuedDisp":"2006-","dateIssuedKey":"2006","publisher":"[Verlag nicht ermittelbar]"}],"titleAlt":[{"title":"JoVE journal"},{"title":"JoVE journal Biology"},{"title":"JoVE journal Medicine"},{"title":"JoVE journal Behavior"},{"title":"JoVE journal Biochemistry"},{"title":"JoVE journal Bioengineering"},{"title":"JoVE journal Cancer research"},{"title":"JoVE journal Chemistry"},{"title":"JoVE journal Developmental biology"},{"title":"JoVE journal Engineering"},{"title":"JoVE journal Environment"},{"title":"JoVE journal Genetics"},{"title":"JoVE journal Immunology and infection"},{"title":"JoVE journal Neuroscience"},{"title":"Journal of visualized experiments"}],"note":["Gesehen am 10.02.25","Inhaltliche Gliederung in: Behavior; Biochemistry; Bioengineering; Biology; Cancer research; Chemistry; Developmental biology; Engineering; Environment; Genetics; Immunology and infection; Medicine; Neuroscience"],"type":{"bibl":"periodical","media":"Online-Ressource"},"pubHistory":["1.2006 -"]}]} 
SRT |a UHLSEBASTICONTRACTIL1720 

Similar Items