In vitro maturation of oocytes is not associated with altered deoxyribonucleic acid methylation patterns in children from in vitro fertilization or intracytoplasmic sperm injection
Objective - To study the possible transmission, to the next generation, of epigenetic defects associated with in vitro maturation (IVM) of human oocytes. - Design - Case-control study using epigenetic data. - Setting - Two collaborating university departments. - Patient(s) - Eleven IVM newborns and...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
January 6, 2015
|
| In: |
Fertility and sterility
Year: 2015, Volume: 103, Issue: 3, Pages: 720-727.e1 |
| ISSN: | 1556-5653 |
| DOI: | 10.1016/j.fertnstert.2014.12.096 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.fertnstert.2014.12.096 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0015028214025217 
|
| Author Notes: | Galyna Pliushch, M.Sc., Eberhard Schneider, Ph.D., Tamara Schneider, Ph.D., Nady El Hajj, Ph.D.,Sabine Rösner, M.D., Thomas Strowitzki, M.D., and Thomas Haaf, M.D. |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1713884909 | ||
| 003 | DE-627 | ||
| 005 | 20240406193238.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200708s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.fertnstert.2014.12.096 |2 doi | |
| 035 | |a (DE-627)1713884909 | ||
| 035 | |a (DE-599)KXP1713884909 | ||
| 035 | |a (OCoLC)1341344872 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Pliushch, Galyna |e VerfasserIn |0 (DE-588)1213352312 |0 (DE-627)1713886464 |4 aut | |
| 245 | 1 | 0 | |a In vitro maturation of oocytes is not associated with altered deoxyribonucleic acid methylation patterns in children from in vitro fertilization or intracytoplasmic sperm injection |c Galyna Pliushch, M.Sc., Eberhard Schneider, Ph.D., Tamara Schneider, Ph.D., Nady El Hajj, Ph.D.,Sabine Rösner, M.D., Thomas Strowitzki, M.D., and Thomas Haaf, M.D. |
| 264 | 1 | |c January 6, 2015 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.07.2020 | ||
| 520 | |a Objective - To study the possible transmission, to the next generation, of epigenetic defects associated with in vitro maturation (IVM) of human oocytes. - Design - Case-control study using epigenetic data. - Setting - Two collaborating university departments. - Patient(s) - Eleven IVM newborns and 19 controls, conceived by conventional assisted reproduction. - Intervention(s) - Chorionic villus and cord-blood sampling. - Main Outcome Measure(s) - Using bisulfite pyrosequencing, we have measured average methylation levels of 6 imprinted (LIT1, MEG, MEST, NESPas, PEG3, and SNRPN), 5 tumor-suppressor (APC, ATM, BRCA1, RAD51C, and TP53), 2 pluripotency (NANOG and OCT4), and 2 metabolic (LEP and NR3C1) genes, as well as 2 repetitive elements (ALU and LINE1) in 2 tissues of IVM and control neonates. Using deep bisulfite sequencing, we have determined methylation patterns of many individual DNA molecules to detect rare RAD51C epimutations (allele methylation errors). - Result(s) - No statistically significant impact was found of IVM on chorionic villus and cord-blood DNA methylation at the studied developmentally important genes and interspersed repeats. The RAD51C epimutation rate was low (0.5% ± 0.1%) in all analyzed samples. - Conclusion(s) - IVM-induced epigenetic changes in offspring, if any, are relatively small in magnitude and/or infrequent. | ||
| 650 | 4 | |a Bisulfite pyrosequencing | |
| 650 | 4 | |a chorionic villus | |
| 650 | 4 | |a cord blood | |
| 650 | 4 | |a deep bisulfite sequencing | |
| 650 | 4 | |a imprinting | |
| 650 | 4 | |a in vitro maturation | |
| 700 | 1 | |a Schneider, Eberhard |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schneider, Tamara |e VerfasserIn |4 aut | |
| 700 | 1 | |a El Hajj, Nady |e VerfasserIn |4 aut | |
| 700 | 1 | |a Rösner, Sabine |d 1965- |e VerfasserIn |0 (DE-588)122346777 |0 (DE-627)705872092 |0 (DE-576)182933687 |4 aut | |
| 700 | 1 | |a Strowitzki, Thomas |d 1959- |e VerfasserIn |0 (DE-588)118055917 |0 (DE-627)079216099 |0 (DE-576)17102141X |4 aut | |
| 700 | 1 | |a Haaf, Thomas |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Fertility and sterility |d Amsterdam [u.a.] : Elsevier Science, 1950 |g 103(2015), 3, Seite 720-727.e1 |h Online-Ressource |w (DE-627)306659492 |w (DE-600)1500469-7 |w (DE-576)121465454 |x 1556-5653 |7 nnas |a In vitro maturation of oocytes is not associated with altered deoxyribonucleic acid methylation patterns in children from in vitro fertilization or intracytoplasmic sperm injection |
| 773 | 1 | 8 | |g volume:103 |g year:2015 |g number:3 |g pages:720-727.e1 |g extent:9 |a In vitro maturation of oocytes is not associated with altered deoxyribonucleic acid methylation patterns in children from in vitro fertilization or intracytoplasmic sperm injection |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.fertnstert.2014.12.096 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0015028214025217 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200708 | ||
| 993 | |a Article | ||
| 994 | |a 2015 | ||
| 998 | |g 118055917 |a Strowitzki, Thomas |m 118055917:Strowitzki, Thomas |d 910000 |d 910400 |e 910000PS118055917 |e 910400PS118055917 |k 0/910000/ |k 1/910000/910400/ |p 6 | ||
| 998 | |g 122346777 |a Rösner, Sabine |m 122346777:Rösner, Sabine |d 910000 |d 910400 |e 910000PR122346777 |e 910400PR122346777 |k 0/910000/ |k 1/910000/910400/ |p 5 | ||
| 999 | |a KXP-PPN1713884909 |e 3711328792 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedKey":"2015","dateIssuedDisp":"January 6, 2015"}],"relHost":[{"origin":[{"dateIssuedKey":"1950","publisherPlace":"Amsterdam [u.a.]","dateIssuedDisp":"1950-","publisher":"Elsevier Science"}],"pubHistory":["1.1950 -"],"type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"note":["Gesehen am 26.01.24","Ungezählte Beil.: Supplement"],"id":{"zdb":["1500469-7"],"eki":["306659492"],"issn":["1556-5653"]},"title":[{"title_sort":"Fertility and sterility","title":"Fertility and sterility"}],"part":{"volume":"103","year":"2015","text":"103(2015), 3, Seite 720-727.e1","extent":"9","issue":"3","pages":"720-727.e1"},"physDesc":[{"extent":"Online-Ressource"}],"recId":"306659492","disp":"In vitro maturation of oocytes is not associated with altered deoxyribonucleic acid methylation patterns in children from in vitro fertilization or intracytoplasmic sperm injectionFertility and sterility"}],"person":[{"display":"Pliushch, Galyna","role":"aut","given":"Galyna","family":"Pliushch"},{"display":"Schneider, Eberhard","family":"Schneider","role":"aut","given":"Eberhard"},{"display":"Schneider, Tamara","family":"Schneider","given":"Tamara","role":"aut"},{"display":"El Hajj, Nady","family":"El Hajj","role":"aut","given":"Nady"},{"display":"Rösner, Sabine","role":"aut","given":"Sabine","family":"Rösner"},{"display":"Strowitzki, Thomas","family":"Strowitzki","role":"aut","given":"Thomas"},{"display":"Haaf, Thomas","given":"Thomas","role":"aut","family":"Haaf"}],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 08.07.2020"],"title":[{"title_sort":"In vitro maturation of oocytes is not associated with altered deoxyribonucleic acid methylation patterns in children from in vitro fertilization or intracytoplasmic sperm injection","title":"In vitro maturation of oocytes is not associated with altered deoxyribonucleic acid methylation patterns in children from in vitro fertilization or intracytoplasmic sperm injection"}],"physDesc":[{"extent":"9 S."}],"recId":"1713884909","name":{"displayForm":["Galyna Pliushch, M.Sc., Eberhard Schneider, Ph.D., Tamara Schneider, Ph.D., Nady El Hajj, Ph.D.,Sabine Rösner, M.D., Thomas Strowitzki, M.D., and Thomas Haaf, M.D."]},"id":{"eki":["1713884909"],"doi":["10.1016/j.fertnstert.2014.12.096"]}} | ||
| SRT | |a PLIUSHCHGAINVITROMAT6201 | ||