Endothelial dysfunction of bypass graft: direct comparison of in Vitro and in vivo models of ischemia-reperfusion injury
Background Although, ischemia/reperfusion induced vascular dysfunction has been widely described, no comparative study of in vivo- and in vitro-models exist. In this study, we provide a direct comparison between models (A) ischemic storage and in-vitro reoxygenation (B) ischemic storage and in vitro...
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| Hauptverfasser: | , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
April 15, 2015
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| In: |
PLOS ONE
Year: 2015, Jahrgang: 10, Heft: 4 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0124025 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0124025 Verlag, lizenzpflichtig, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0124025 
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| Verfasserangaben: | Gábor Veres, Péter Hegedűs, Enikő Barnucz, Raphael Zöller, Stephanie Klein, Harald Schmidt, Tamás Radovits, Sevil Korkmaz, Matthias Karck, Gábor Szabó |
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| 245 | 1 | 0 | |a Endothelial dysfunction of bypass graft |b direct comparison of in Vitro and in vivo models of ischemia-reperfusion injury |c Gábor Veres, Péter Hegedűs, Enikő Barnucz, Raphael Zöller, Stephanie Klein, Harald Schmidt, Tamás Radovits, Sevil Korkmaz, Matthias Karck, Gábor Szabó |
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| 520 | |a Background Although, ischemia/reperfusion induced vascular dysfunction has been widely described, no comparative study of in vivo- and in vitro-models exist. In this study, we provide a direct comparison between models (A) ischemic storage and in-vitro reoxygenation (B) ischemic storage and in vitro reperfusion (C) ischemic storage and in-vivo reperfusion. Methods and Results Aortic arches from rats were stored for 2 hours in saline. Arches were then (A) in vitro reoxygenated (B) in vitro incubated in hypochlorite for 30 minutes (C) in vivo reperfused after heterotransplantation (2, 24 hours and 7 days reperfusion). Endothelium-dependent and independent vasorelaxations were assessed in organ bath. DNA strand breaks were assessed by TUNEL-method, mRNA expressions (caspase-3, bax, bcl-2, eNOS) by quantitative real-time PCR, proteins by Western blot analysis and the expression of CD-31 by immunochemistry. Endothelium-dependent maximal relaxation was drastically reduced in the in-vivo models compared to ischemic storage and in-vitro reperfusion group, and no difference showed between ischemic storage and control group. CD31-staining showed significantly lower endothelium surface ratio in-vivo, which correlated with TUNEL-positive ratio. Increased mRNA and protein levels of pro- and anti-apoptotic gens indicated a significantly higher damage in the in-vivo models. Conclusion Even short-period of ischemia induces severe endothelial damage (in-vivo reperfusion model). In-vitro models of ischemia-reperfusion injury can be limitedly suited for reliable investigations. Time course of endothelial stunning is also described. | ||
| 650 | 4 | |a Coronary artery bypass grafting | |
| 650 | 4 | |a Endothelial cells | |
| 650 | 4 | |a Endothelium | |
| 650 | 4 | |a Gene expression | |
| 650 | 4 | |a Hypochlorites | |
| 650 | 4 | |a Ischemia | |
| 650 | 4 | |a Reperfusion | |
| 650 | 4 | |a Reperfusion injury | |
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