Drug target identification using a trypanosome overexpression library
Elucidation of molecular targets is very important for lead optimization during the drug development process. We describe a direct method to find targets of antitrypanosomal compounds against Trypanosoma brucei using a trypanosome overexpression library. As proof of concept, we treated the library w...
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| Hauptverfasser: | , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
21 July 2014
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| In: |
Antimicrobial agents and chemotherapy
Year: 2014, Jahrgang: 58, Heft: 10, Pages: 6260-6264 |
| ISSN: | 1098-6596 |
| DOI: | 10.1128/AAC.03338-14 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1128/AAC.03338-14 Verlag, lizenzpflichtig, Volltext: https://aac.asm.org/content/58/10/6260 
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| Verfasserangaben: | Daniela Begolo, Esteban Erben, Christine Clayton |
| Zusammenfassung: | Elucidation of molecular targets is very important for lead optimization during the drug development process. We describe a direct method to find targets of antitrypanosomal compounds against Trypanosoma brucei using a trypanosome overexpression library. As proof of concept, we treated the library with difluoromethylornithine and DDD85646 and identified their respective targets, ornithine decarboxylase and N-myristoyltransferase. The overexpression library could be a useful tool to study the modes of action of novel antitrypanosomal drug candidates. |
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| Beschreibung: | Gesehen am 15.07.2020 |
| Beschreibung: | Online Resource |
| ISSN: | 1098-6596 |
| DOI: | 10.1128/AAC.03338-14 |