Dual single-scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered β2-adrenergic receptor (β2AR) or Mu-opioid receptor (MOR) endocytosis
The dynamic relationship between constitutive and ligand-triggered clathrin-mediated endocytosis is only poorly characterized, and it remains controversial whether clathrin-coated pits specialize to internalize particular receptor cargo. Here we analyzed the ligand-triggered endocytosis of the model...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
July 30, 2014
|
| In: |
Molecular biology of the cell
Year: 2014, Jahrgang: 25, Heft: 19, Pages: 3070-3080 |
| ISSN: | 1939-4586 |
| DOI: | 10.1091/mbc.e14-06-1112 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1091/mbc.e14-06-1112 Verlag, lizenzpflichtig, Volltext: https://www.molbiolcell.org/doi/10.1091/mbc.e14-06-1112 
|
| Verfasserangaben: | Marko Lampe, Fabienne Pierre, Suleiman Al-Sabah, Cornelius Krasel, and Christien J. Merrifield |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1725166291 | ||
| 003 | DE-627 | ||
| 005 | 20220818152933.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200720s2014 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1091/mbc.e14-06-1112 |2 doi | |
| 035 | |a (DE-627)1725166291 | ||
| 035 | |a (DE-599)KXP1725166291 | ||
| 035 | |a (OCoLC)1341346895 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Lampe, Marko |e VerfasserIn |0 (DE-588)133965651 |0 (DE-627)558839460 |0 (DE-576)300221843 |4 aut | |
| 245 | 1 | 0 | |a Dual single-scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered β2-adrenergic receptor (β2AR) or Mu-opioid receptor (MOR) endocytosis |c Marko Lampe, Fabienne Pierre, Suleiman Al-Sabah, Cornelius Krasel, and Christien J. Merrifield |
| 246 | 3 | 3 | |a Dual single-scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered beta 2-adrenergic receptor ( beta 2AR) or Mu-opioid receptor (MOR) endocytosis |
| 264 | 1 | |c July 30, 2014 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 20.07.2020 | ||
| 520 | |a The dynamic relationship between constitutive and ligand-triggered clathrin-mediated endocytosis is only poorly characterized, and it remains controversial whether clathrin-coated pits specialize to internalize particular receptor cargo. Here we analyzed the ligand-triggered endocytosis of the model G-protein-coupled receptors (GPCRs) β2-adrenergic receptor (β2AR) and Mu-opioid receptor (MOR) at the level of individual endocytic events using a total internal reflection fluorescence microscopy (TIRFM)-based assay. Similar to the constitutive endocytosis of transferrin receptor (TfR), ligand- triggered endocytosis of β2AR occurs via quantized scission events hosted by clathrin spots and plaques of variable size and persistence. To address whether clathrin-coated structures (CCSs) specialize to internalize particular GPCRs, we adapted the TIRFM imaging assay to simultaneously quantify the internalization of TfR and the ligand- triggered endocytosis of the β2AR or MOR. Agonist-triggered β2AR or MOR endocytosis extended the maturation time of CCSs, as shown previously, but did not affect the rate of constitutive TfR endocytosis or loading of TfR into individual endocytic vesicles. Both the β2AR and the MOR receptors entered cells in the same vesicles as TfR, and the overall evidence for CCS specialization was weak. These data support a simple model in which different cargoes internalize through common CCSs. | ||
| 700 | 1 | |a Pierre, Fabienne |e VerfasserIn |4 aut | |
| 700 | 1 | |a Al-Sabah, Suleiman |e VerfasserIn |4 aut | |
| 700 | 1 | |a Krasel, Cornelius |e VerfasserIn |4 aut | |
| 700 | 1 | |a Merrifield, Christien J. |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular biology of the cell |d Bethesda, Md. : American Society for Cell Biology, 1992 |g 25(2014), 19, Seite 3070-3080 |h Online-Ressource |w (DE-627)269533680 |w (DE-600)1474922-1 |w (DE-576)077884361 |x 1939-4586 |7 nnas |a Dual single-scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered β2-adrenergic receptor (β2AR) or Mu-opioid receptor (MOR) endocytosis |
| 773 | 1 | 8 | |g volume:25 |g year:2014 |g number:19 |g pages:3070-3080 |g extent:11 |a Dual single-scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered β2-adrenergic receptor (β2AR) or Mu-opioid receptor (MOR) endocytosis |
| 856 | 4 | 0 | |u https://doi.org/10.1091/mbc.e14-06-1112 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.molbiolcell.org/doi/10.1091/mbc.e14-06-1112 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200720 | ||
| 993 | |a Article | ||
| 994 | |a 2014 | ||
| 998 | |g 133965651 |a Lampe, Marko |m 133965651:Lampe, Marko |p 1 |x j | ||
| 999 | |a KXP-PPN1725166291 |e 3725999716 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 20.07.2020"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"recId":"1725166291","titleAlt":[{"title":"Dual single-scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered beta 2-adrenergic receptor ( beta 2AR) or Mu-opioid receptor (MOR) endocytosis"}],"person":[{"given":"Marko","family":"Lampe","role":"aut","roleDisplay":"VerfasserIn","display":"Lampe, Marko"},{"roleDisplay":"VerfasserIn","display":"Pierre, Fabienne","role":"aut","family":"Pierre","given":"Fabienne"},{"given":"Suleiman","family":"Al-Sabah","role":"aut","roleDisplay":"VerfasserIn","display":"Al-Sabah, Suleiman"},{"family":"Krasel","given":"Cornelius","display":"Krasel, Cornelius","roleDisplay":"VerfasserIn","role":"aut"},{"display":"Merrifield, Christien J.","roleDisplay":"VerfasserIn","role":"aut","family":"Merrifield","given":"Christien J."}],"title":[{"title_sort":"Dual single-scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered β2-adrenergic receptor (β2AR) or Mu-opioid receptor (MOR) endocytosis","title":"Dual single-scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered β2-adrenergic receptor (β2AR) or Mu-opioid receptor (MOR) endocytosis"}],"physDesc":[{"extent":"11 S."}],"relHost":[{"pubHistory":["3.1992 -"],"part":{"text":"25(2014), 19, Seite 3070-3080","volume":"25","extent":"11","year":"2014","issue":"19","pages":"3070-3080"},"titleAlt":[{"title":"MBC online"},{"title":"MBoC"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Dual single-scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered β2-adrenergic receptor (β2AR) or Mu-opioid receptor (MOR) endocytosisMolecular biology of the cell","note":["Gesehen am 07.09.12"],"recId":"269533680","corporate":[{"role":"isb","display":"American Society for Cell Biology","roleDisplay":"Herausgebendes Organ"}],"language":["eng"],"title":[{"title_sort":"Molecular biology of the cell","subtitle":"the official publication of the American Society for Cell Biology","title":"Molecular biology of the cell"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisherPlace":"Bethesda, Md.","dateIssuedKey":"1992","publisher":"American Society for Cell Biology","dateIssuedDisp":"1992-"}],"id":{"issn":["1939-4586"],"zdb":["1474922-1"],"eki":["269533680"]}}],"name":{"displayForm":["Marko Lampe, Fabienne Pierre, Suleiman Al-Sabah, Cornelius Krasel, and Christien J. Merrifield"]},"origin":[{"dateIssuedDisp":"July 30, 2014","dateIssuedKey":"2014"}],"id":{"doi":["10.1091/mbc.e14-06-1112"],"eki":["1725166291"]}} | ||
| SRT | |a LAMPEMARKODUALSINGLE3020 | ||