Impaired suppressive capacity of activation-induced regulatory B cells in systemic lupus erythematosus
Objective B cells with immunoregulatory properties (Breg cells) have been described in mice, but their role in the control of human immune responses is not well defined. We recently identified a human population of activated FSChigh B cells that exhibited regulatory activity toward T helper cells. T...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
18 June 2014
|
| In: |
Arthritis & rheumatology
Year: 2014, Jahrgang: 66, Heft: 10, Pages: 2849-2861 |
| ISSN: | 2326-5205 |
| DOI: | 10.1002/art.38742 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/art.38742 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/art.38742 
|
| Verfasserangaben: | Nele Gao, Julia Dresel, Volker Eckstein, Rimma Gellert, Hannah Störch, Ram K. C. Venigalla, Vedat Schwenger, Regina Max, Norbert Blank, Hanns-Martin Lorenz, and Theresa Tretter |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1725204835 | ||
| 003 | DE-627 | ||
| 005 | 20230426103401.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200721s2014 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/art.38742 |2 doi | |
| 035 | |a (DE-627)1725204835 | ||
| 035 | |a (DE-599)KXP1725204835 | ||
| 035 | |a (OCoLC)1341347097 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Gao, Nele Xian-Tai |d 1985- |e VerfasserIn |0 (DE-588)1054104360 |0 (DE-627)791038289 |0 (DE-576)409997498 |4 aut | |
| 245 | 1 | 0 | |a Impaired suppressive capacity of activation-induced regulatory B cells in systemic lupus erythematosus |c Nele Gao, Julia Dresel, Volker Eckstein, Rimma Gellert, Hannah Störch, Ram K. C. Venigalla, Vedat Schwenger, Regina Max, Norbert Blank, Hanns-Martin Lorenz, and Theresa Tretter |
| 264 | 1 | |c 18 June 2014 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 21.07.2020 | ||
| 520 | |a Objective B cells with immunoregulatory properties (Breg cells) have been described in mice, but their role in the control of human immune responses is not well defined. We recently identified a human population of activated FSChigh B cells that exhibited regulatory activity toward T helper cells. The aim of the present study was to test such induced Breg (iBreg) cells in patients with autoimmune disease. Methods Purified CD19+FSChigh B cells derived from patients with systemic lupus erythematosus (SLE) or from healthy donors, which were activated via their B cell receptor, were cocultured with CD3-stimulated CD4+ T helper cells from SLE patients or healthy donors. 3H-thymidine incorporation, flow cytometry, and enzyme-linked immunosorbent assay (ELISA) were used to analyze proliferation, cytokine secretion, and surface marker expression. Results Although under costimulatory conditions, FSChigh SLE B cells supported the proliferation of healthy donor T cells to a similar extent as donor B cells, their regulatory function was significantly diminished in B cell suppressor assays. Similar effects were seen when SLE T cells were used, confirming that SLE T cells were equally susceptible to iBreg cell signals as healthy donor T cells and that SLE iBreg cell defects were independent of T cell origin. B cell viability and expression of surface markers (CD25, CD80, and B7-H1) or cytokines (interleukin-6 [IL-6], tumor necrosis factor α, and IL-10) were comparable in the two B cell populations. There was no correlation between the extent of iBreg cell-induced inhibition and disease activity. CD19+FSChigh B cells from patients with another systemic autoimmune disease, granulomatosis with polyangiitis (Wegener's) (GPA), exhibited no regulatory defects, which suggests that the iBreg cell defects were SLE-specific and not a general consequence of autoimmunity or inflammation. Conclusion Induced Breg cells from SLE patients, but not GPA patients, are less effective in the control of T helper cell proliferation, which supports the reported skewed B cell repertoire in SLE. The malfunctioning SLE iBreg cells might allow the overstimulation of immune responses and contribute to the initiation and/or perpetuation of disease. | ||
| 700 | 1 | |a Dresel, Julia |d 1979- |e VerfasserIn |0 (DE-588)133047172 |0 (DE-627)691110263 |0 (DE-576)265604451 |4 aut | |
| 700 | 1 | |a Eckstein, Volker |d 1957- |e VerfasserIn |0 (DE-588)172851408 |0 (DE-627)697780732 |0 (DE-576)133707059 |4 aut | |
| 700 | 1 | |a Gellert, Rimma |e VerfasserIn |0 (DE-588)1214255310 |0 (DE-627)1725205483 |4 aut | |
| 700 | 1 | |a Ostermann, Hannah |d 1987- |e VerfasserIn |0 (DE-588)1095253476 |0 (DE-627)855929995 |0 (DE-576)462945316 |4 aut | |
| 700 | 1 | |a Venigalla, Ram Kumar Chowdary |d 1974- |e VerfasserIn |0 (DE-588)137181507 |0 (DE-627)694935506 |0 (DE-576)302389733 |4 aut | |
| 700 | 1 | |a Schwenger, Vedat |e VerfasserIn |0 (DE-588)1022867652 |0 (DE-627)717320197 |0 (DE-576)176592237 |4 aut | |
| 700 | 1 | |a Max, Regina |e VerfasserIn |0 (DE-588)1050991001 |0 (DE-627)785478418 |0 (DE-576)176590609 |4 aut | |
| 700 | 1 | |a Blank, Norbert |d 1964- |e VerfasserIn |0 (DE-588)115586032 |0 (DE-627)691431507 |0 (DE-576)289963508 |4 aut | |
| 700 | 1 | |a Lorenz, Hanns-Martin |d 1962- |e VerfasserIn |0 (DE-588)112253865 |0 (DE-627)627277845 |0 (DE-576)167601369 |4 aut | |
| 700 | 1 | |a Tretter, Theresa |e VerfasserIn |0 (DE-588)1083287559 |0 (DE-627)84793179X |0 (DE-576)455679983 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Arthritis & rheumatology |d Hoboken, NJ : Wiley, 2014 |g 66(2014), 10, Seite 2849-2861 |h Online-Ressource |w (DE-627)777780615 |w (DE-600)2754614-7 |w (DE-576)400527065 |x 2326-5205 |7 nnas |a Impaired suppressive capacity of activation-induced regulatory B cells in systemic lupus erythematosus |
| 773 | 1 | 8 | |g volume:66 |g year:2014 |g number:10 |g pages:2849-2861 |g extent:13 |a Impaired suppressive capacity of activation-induced regulatory B cells in systemic lupus erythematosus |
| 856 | 4 | 0 | |u https://doi.org/10.1002/art.38742 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://onlinelibrary.wiley.com/doi/abs/10.1002/art.38742 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200721 | ||
| 993 | |a Article | ||
| 994 | |a 2014 | ||
| 998 | |g 1083287559 |a Tretter, Theresa |m 1083287559:Tretter, Theresa |d 910000 |d 910100 |e 910000PT1083287559 |e 910100PT1083287559 |k 0/910000/ |k 1/910000/910100/ |p 11 |y j | ||
| 998 | |g 112253865 |a Lorenz, Hanns-Martin |m 112253865:Lorenz, Hanns-Martin |d 910000 |d 910100 |e 910000PL112253865 |e 910100PL112253865 |k 0/910000/ |k 1/910000/910100/ |p 10 | ||
| 998 | |g 115586032 |a Blank, Norbert |m 115586032:Blank, Norbert |d 910000 |d 910100 |e 910000PB115586032 |e 910100PB115586032 |k 0/910000/ |k 1/910000/910100/ |p 9 | ||
| 998 | |g 1050991001 |a Max, Regina |m 1050991001:Max, Regina |d 910000 |d 910100 |e 910000PM1050991001 |e 910100PM1050991001 |k 0/910000/ |k 1/910000/910100/ |p 8 | ||
| 998 | |g 1022867652 |a Schwenger, Vedat |m 1022867652:Schwenger, Vedat |d 50000 |e 50000PS1022867652 |k 0/50000/ |p 7 | ||
| 998 | |g 137181507 |a Venigalla, Ram Kumar Chowdary |m 137181507:Venigalla, Ram Kumar Chowdary |p 6 | ||
| 998 | |g 1095253476 |a Ostermann, Hannah |m 1095253476:Ostermann, Hannah |p 5 | ||
| 998 | |g 1214255310 |a Gellert, Rimma |m 1214255310:Gellert, Rimma |p 4 | ||
| 998 | |g 172851408 |a Eckstein, Volker |m 172851408:Eckstein, Volker |d 910000 |d 910100 |e 910000PE172851408 |e 910100PE172851408 |k 0/910000/ |k 1/910000/910100/ |p 3 | ||
| 998 | |g 133047172 |a Dresel, Julia |m 133047172:Dresel, Julia |p 2 | ||
| 998 | |g 1054104360 |a Gao, Nele Xian-Tai |m 1054104360:Gao, Nele Xian-Tai |p 1 |x j | ||
| 999 | |a KXP-PPN1725204835 |e 3726943587 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 21.07.2020"],"recId":"1725204835","name":{"displayForm":["Nele Gao, Julia Dresel, Volker Eckstein, Rimma Gellert, Hannah Störch, Ram K. C. Venigalla, Vedat Schwenger, Regina Max, Norbert Blank, Hanns-Martin Lorenz, and Theresa Tretter"]},"relHost":[{"part":{"year":"2014","extent":"13","issue":"10","volume":"66","pages":"2849-2861","text":"66(2014), 10, Seite 2849-2861"},"titleAlt":[{"title":"Arthritis and rheumatology"}],"title":[{"subtitle":"an official journal of the American College of Rheumatology","title":"Arthritis & rheumatology","title_sort":"Arthritis & rheumatology"}],"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"corporate":[{"role":"isb","display":"American College of Rheumatology"}],"id":{"eki":["777780615"],"zdb":["2754614-7"],"issn":["2326-5205"]},"type":{"media":"Online-Ressource","bibl":"periodical"},"origin":[{"publisherPlace":"Hoboken, NJ","dateIssuedDisp":"2014-","dateIssuedKey":"2014","publisher":"Wiley"}],"recId":"777780615","pubHistory":["66.2014 -"],"disp":"Impaired suppressive capacity of activation-induced regulatory B cells in systemic lupus erythematosusArthritis & rheumatology"}],"physDesc":[{"extent":"13 S."}],"person":[{"role":"aut","family":"Gao","display":"Gao, Nele Xian-Tai","given":"Nele Xian-Tai"},{"family":"Dresel","display":"Dresel, Julia","given":"Julia","role":"aut"},{"role":"aut","given":"Volker","display":"Eckstein, Volker","family":"Eckstein"},{"role":"aut","given":"Rimma","family":"Gellert","display":"Gellert, Rimma"},{"role":"aut","given":"Hannah","family":"Ostermann","display":"Ostermann, Hannah"},{"role":"aut","given":"Ram Kumar Chowdary","display":"Venigalla, Ram Kumar Chowdary","family":"Venigalla"},{"given":"Vedat","display":"Schwenger, Vedat","family":"Schwenger","role":"aut"},{"given":"Regina","family":"Max","display":"Max, Regina","role":"aut"},{"family":"Blank","display":"Blank, Norbert","given":"Norbert","role":"aut"},{"given":"Hanns-Martin","display":"Lorenz, Hanns-Martin","family":"Lorenz","role":"aut"},{"given":"Theresa","family":"Tretter","display":"Tretter, Theresa","role":"aut"}],"title":[{"title":"Impaired suppressive capacity of activation-induced regulatory B cells in systemic lupus erythematosus","title_sort":"Impaired suppressive capacity of activation-induced regulatory B cells in systemic lupus erythematosus"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"id":{"eki":["1725204835"],"doi":["10.1002/art.38742"]},"language":["eng"],"origin":[{"dateIssuedKey":"2014","dateIssuedDisp":"18 June 2014"}]} | ||
| SRT | |a GAONELEXIAIMPAIREDSU1820 | ||