Cover Image

The mitochondrial peroxiredoxin displays distinct roles in different developmental stages of African trypanosomes

Hydroperoxide reduction in African trypanosomes relies on 2-Cys-peroxiredoxins (Prxs) and glutathione peroxidase-type enzymes (Pxs) which both obtain their reducing equivalents from the trypanothione/tryparedoxin couple and thus act as tryparedoxin peroxidases. While the cytosolic forms of the perox...

Full description

Saved in:
Bibliographic Details
Main Authors: Bogacz, Marta (Author) , Dirdjaja, Natalie (Author) , Wimmer, Benedikt (Author) , Habich, Carina (Author) , Krauth-Siegel, Renate (Author)
Format: Article (Journal)
Language:English
Published: 29 April 2020
In: Redox Biology
Year: 2020, Volume: 34
ISSN:2213-2317
DOI:10.1016/j.redox.2020.101547
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.redox.2020.101547
Get full text
Author Notes:Marta Bogacz, Natalie Dirdjaja, Benedikt Wimmer, Carina Habich, R. Luise Krauth-Siegel

MARC

LEADER 00000caa a2200000 c 4500
001 1725374897
003 DE-627
005 20240406193242.0
007 cr uuu---uuuuu
008 200723s2020 xx |||||o 00| ||eng c
024 7 |a 10.1016/j.redox.2020.101547  |2 doi 
035 |a (DE-627)1725374897 
035 |a (DE-599)KXP1725374897 
035 |a (OCoLC)1341347568 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Bogacz, Marta  |e VerfasserIn  |0 (DE-588)1171486308  |0 (DE-627)1040554938  |0 (DE-576)513753931  |4 aut 
245 1 4 |a The mitochondrial peroxiredoxin displays distinct roles in different developmental stages of African trypanosomes  |c Marta Bogacz, Natalie Dirdjaja, Benedikt Wimmer, Carina Habich, R. Luise Krauth-Siegel 
264 1 |c 29 April 2020 
300 |a 15 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 23.07.2020 
520 |a Hydroperoxide reduction in African trypanosomes relies on 2-Cys-peroxiredoxins (Prxs) and glutathione peroxidase-type enzymes (Pxs) which both obtain their reducing equivalents from the trypanothione/tryparedoxin couple and thus act as tryparedoxin peroxidases. While the cytosolic forms of the peroxidases are essential, the mitochondrial mPrx and Px III appear dispensable in bloodstream Trypanosoma brucei. This led to the suggestion that in this developmental stage which is characterized by a mitochondrion that lacks an active respiratory chain, only one of the two peroxidases might be required. Here we show that bloodstream cells in which the Px III gene is deleted and mPrx is down-regulated by RNA interference, proliferate as the parental cells indicating that both mitochondrial peroxidases are dispensable. However, when we raised the culture temperature to 39 °C, mPrx-depleted cells died indicating that under conditions mimicking a fever situation in the mammalian host, the protein becomes essential. In contrast, depletion of mPrx in insect stage procyclic T. brucei causes a proliferation defect under standard conditions at 27 °C, in the absence of any stress. In the absence of mPrx, a tryparedoxin-coupled roGFP2 biosensor expressed in the mitochondrial matrix is unable to respond to antimycin A treatment. Thus mPrx reduces mitochondrial H2O2 with the generation of trypanothione disulfide and acts as peroxidase. However, mPrx-depleted procyclic cells neither display any alteration in the cytosolic or mitochondrial trypanothione redox state nor increased sensitivity towards exogenous oxidative stressors suggesting that the peroxidase activity is not the crucial physiological function. After prolonged mPrx-depletion, the cells almost stop proliferation and display a highly elongated shape and diminished MitoTracker Red staining. In contrast to the situation in the mammalian bloodstream T. brucei and Leishmania, mPrx appears to play a constitutive role for the morphology, mitochondrial function and proliferation of the insect stage of African trypanosomes. 
650 4 |a Mitochondrion 
650 4 |a Peroxiredoxin 
650 4 |a roGFP2 
650 4 |a Trypanosoma brucei 
650 4 |a Trypanothione 
650 4 |a Tryparedoxin 
700 1 |a Dirdjaja, Natalie  |e VerfasserIn  |0 (DE-588)1100399550  |0 (DE-627)859361314  |0 (DE-576)469692405  |4 aut 
700 1 |a Wimmer, Benedikt  |e VerfasserIn  |0 (DE-588)1214391206  |0 (DE-627)1725379341  |4 aut 
700 1 |a Habich, Carina  |e VerfasserIn  |4 aut 
700 1 |a Krauth-Siegel, Renate  |e VerfasserIn  |0 (DE-588)1050022335  |0 (DE-627)783217390  |0 (DE-576)404300200  |4 aut 
773 0 8 |i Enthalten in  |t Redox Biology  |d Amsterdam [u.a.] : Elsevier, 2013  |g 34(2020) Artikel-Nummer 101547, 15 Seiten  |h Online-Ressource  |w (DE-627)735687307  |w (DE-600)2701011-9  |w (DE-576)378492055  |x 2213-2317  |7 nnas  |a The mitochondrial peroxiredoxin displays distinct roles in different developmental stages of African trypanosomes 
773 1 8 |g volume:34  |g year:2020  |g extent:15  |a The mitochondrial peroxiredoxin displays distinct roles in different developmental stages of African trypanosomes 
856 4 0 |u https://doi.org/10.1016/j.redox.2020.101547  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20200723 
993 |a Article 
994 |a 2020 
998 |g 1050022335  |a Krauth-Siegel, Renate  |m 1050022335:Krauth-Siegel, Renate  |d 700000  |d 711000  |e 700000PK1050022335  |e 711000PK1050022335  |k 0/700000/  |k 1/700000/711000/  |p 5  |y j 
998 |g 1214391206  |a Wimmer, Benedikt  |m 1214391206:Wimmer, Benedikt  |p 3 
998 |g 1100399550  |a Dirdjaja, Natalie  |m 1100399550:Dirdjaja, Natalie  |d 700000  |d 711000  |e 700000PD1100399550  |e 711000PD1100399550  |k 0/700000/  |k 1/700000/711000/  |p 2 
998 |g 1171486308  |a Bogacz, Marta  |m 1171486308:Bogacz, Marta  |p 1  |x j 
999 |a KXP-PPN1725374897  |e 3728828300 
BIB |a Y 
SER |a journal 
JSO |a {"person":[{"given":"Marta","family":"Bogacz","role":"aut","display":"Bogacz, Marta","roleDisplay":"VerfasserIn"},{"given":"Natalie","family":"Dirdjaja","role":"aut","roleDisplay":"VerfasserIn","display":"Dirdjaja, Natalie"},{"family":"Wimmer","given":"Benedikt","display":"Wimmer, Benedikt","roleDisplay":"VerfasserIn","role":"aut"},{"role":"aut","display":"Habich, Carina","roleDisplay":"VerfasserIn","given":"Carina","family":"Habich"},{"given":"Renate","family":"Krauth-Siegel","role":"aut","display":"Krauth-Siegel, Renate","roleDisplay":"VerfasserIn"}],"title":[{"title":"The mitochondrial peroxiredoxin displays distinct roles in different developmental stages of African trypanosomes","title_sort":"mitochondrial peroxiredoxin displays distinct roles in different developmental stages of African trypanosomes"}],"note":["Gesehen am 23.07.2020"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"recId":"1725374897","language":["eng"],"name":{"displayForm":["Marta Bogacz, Natalie Dirdjaja, Benedikt Wimmer, Carina Habich, R. Luise Krauth-Siegel"]},"origin":[{"dateIssuedDisp":"29 April 2020","dateIssuedKey":"2020"}],"id":{"doi":["10.1016/j.redox.2020.101547"],"eki":["1725374897"]},"physDesc":[{"extent":"15 S."}],"relHost":[{"pubHistory":["1.2013 -"],"part":{"year":"2020","text":"34(2020) Artikel-Nummer 101547, 15 Seiten","volume":"34","extent":"15"},"disp":"The mitochondrial peroxiredoxin displays distinct roles in different developmental stages of African trypanosomesRedox Biology","note":["Gesehen am 13.08.20"],"type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"recId":"735687307","title":[{"subtitle":"an official journal of the Society for Free Radical Biology and Medicine, an official journal of the Society for Free Radical Research-Europe, an affiliate journal of the International Society for Free Radical Research (SFRRI)","title":"Redox Biology","title_sort":"Redox Biology"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"dateIssuedKey":"2013","publisher":"Elsevier","dateIssuedDisp":"2013-","publisherPlace":"Amsterdam [u.a.]"}],"id":{"issn":["2213-2317"],"eki":["735687307"],"zdb":["2701011-9"]}}]} 
SRT |a BOGACZMARTMITOCHONDR2920 

Similar Items