Proximity mapping of human separase by the BioID approach

Separase is a caspase-like cysteine protease that is best known for its essential role during the metaphase-to-anaphase transition when it cleaves the cohesin ring complex that keeps the sister chromatids together. Another important function of separase is to regulate the process of centriole separa...

Full description

Saved in:
Bibliographic Details
Main Authors: Agircan, Fikret Gürkan (Author) , Hata, Shoji (Author) , Nussbaum-Krammer, Carmen (Author) , Atorino, Enrico (Author) , Schiebel, Elmar (Author)
Format: Article (Journal)
Language:English
Published: 3 August 2016
In: Biochemical and biophysical research communications
Year: 2016, Volume: 478, Issue: 2, Pages: 656-662
ISSN:1090-2104
DOI:10.1016/j.bbrc.2016.08.002
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.bbrc.2016.08.002
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0006291X16312578
Get full text
Author Notes:Fikret Gurkan Agircan, Shoji Hata, Carmen Nussbaum-Krammer, Enrico Atorino, Elmar Schiebel

MARC

LEADER 00000caa a2200000 c 4500
001 1726783871
003 DE-627
005 20220818173210.0
007 cr uuu---uuuuu
008 200811s2016 xx |||||o 00| ||eng c
024 7 |a 10.1016/j.bbrc.2016.08.002  |2 doi 
035 |a (DE-627)1726783871 
035 |a (DE-599)KXP1726783871 
035 |a (OCoLC)1341355025 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Agircan, Fikret Gürkan  |e VerfasserIn  |0 (DE-588)1113301783  |0 (DE-627)867166355  |0 (DE-576)476943124  |4 aut 
245 1 0 |a Proximity mapping of human separase by the BioID approach  |c Fikret Gurkan Agircan, Shoji Hata, Carmen Nussbaum-Krammer, Enrico Atorino, Elmar Schiebel 
264 1 |c 3 August 2016 
300 |a 7 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 11.08.2020 
520 |a Separase is a caspase-like cysteine protease that is best known for its essential role during the metaphase-to-anaphase transition when it cleaves the cohesin ring complex that keeps the sister chromatids together. Another important function of separase is to regulate the process of centriole separation, known as centriole disengagement, at the end of mitosis. We used proximity-dependent biotin identification (BioID) to expand our knowledge on the identity of separase's proximity interactors. We show that separase BioID labeled two domains at the mother centriole: an area underneath the centriolar appendages and another at the proximal end of the mother centriole. BioID analysis identified more than 200 proximity interactors of separase, one being the Alström Syndrome Protein 1 (ALMS1) at the base of centrioles. Other proximity interactors are the histone chaperons NAP1L1 and NAP1L4, which localize to the spindle poles during mitosis and the spindle assembly checkpoint proteins BUBR1, SKA1 and SKA3 that reside at kinetochores in early mitosis. Finally, we show that depletion of BUBR1 homolog from Caenorhabditis elegans delayed the recruitment of separase to mitotic chromosomes, and eventually anaphase onset. 
650 4 |a BioID 
650 4 |a Centrosome 
650 4 |a Kinetochore 
650 4 |a Separase 
700 1 |a Hata, Shoji  |e VerfasserIn  |0 (DE-588)1196704600  |0 (DE-627)1678544078  |4 aut 
700 1 |a Nussbaum-Krammer, Carmen  |e VerfasserIn  |0 (DE-588)107836446X  |0 (DE-627)838428819  |0 (DE-576)450764745  |4 aut 
700 1 |a Atorino, Enrico  |d 1990-  |e VerfasserIn  |0 (DE-588)1196705089  |0 (DE-627)1678544434  |4 aut 
700 1 |a Schiebel, Elmar  |d 1960-  |e VerfasserIn  |0 (DE-588)1034622595  |0 (DE-627)746029462  |0 (DE-576)165868597  |4 aut 
773 0 8 |i Enthalten in  |t Biochemical and biophysical research communications  |d [Amsterdam] : Elsevier B.V., 1959  |g 478(2016), 2, Seite 656-662  |h Online-Ressource  |w (DE-627)254231691  |w (DE-600)1461396-7  |w (DE-576)103373039  |x 1090-2104  |7 nnas  |a Proximity mapping of human separase by the BioID approach 
773 1 8 |g volume:478  |g year:2016  |g number:2  |g pages:656-662  |g extent:7  |a Proximity mapping of human separase by the BioID approach 
856 4 0 |u https://doi.org/10.1016/j.bbrc.2016.08.002  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u http://www.sciencedirect.com/science/article/pii/S0006291X16312578  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20200811 
993 |a Article 
994 |a 2016 
998 |g 1034622595  |a Schiebel, Elmar  |m 1034622595:Schiebel, Elmar  |d 700000  |d 706000  |e 700000PS1034622595  |e 706000PS1034622595  |k 0/700000/  |k 1/700000/706000/  |p 5  |y j 
998 |g 1196705089  |a Atorino, Enrico  |m 1196705089:Atorino, Enrico  |d 700000  |d 706000  |e 700000PA1196705089  |e 706000PA1196705089  |k 0/700000/  |k 1/700000/706000/  |p 4 
998 |g 107836446X  |a Nussbaum-Krammer, Carmen  |m 107836446X:Nussbaum-Krammer, Carmen  |d 700000  |d 706000  |e 700000PN107836446X  |e 706000PN107836446X  |k 0/700000/  |k 1/700000/706000/  |p 3 
998 |g 1196704600  |a Hata, Shoji  |m 1196704600:Hata, Shoji  |d 700000  |d 706000  |e 700000PH1196704600  |e 706000PH1196704600  |k 0/700000/  |k 1/700000/706000/  |p 2 
998 |g 1113301783  |a Agircan, Fikret Gürkan  |m 1113301783:Agircan, Fikret Gürkan  |d 700000  |d 706000  |e 700000PA1113301783  |e 706000PA1113301783  |k 0/700000/  |k 1/700000/706000/  |p 1  |x j 
999 |a KXP-PPN1726783871  |e 3738031928 
BIB |a Y 
SER |a journal 
JSO |a {"origin":[{"dateIssuedDisp":"3 August 2016","dateIssuedKey":"2016"}],"name":{"displayForm":["Fikret Gurkan Agircan, Shoji Hata, Carmen Nussbaum-Krammer, Enrico Atorino, Elmar Schiebel"]},"relHost":[{"id":{"zdb":["1461396-7"],"issn":["1090-2104"],"eki":["254231691"]},"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"recId":"254231691","titleAlt":[{"title":"BBRC"}],"note":["Gesehen am 14.06.2024"],"title":[{"title":"Biochemical and biophysical research communications","subtitle":"BBRC","title_sort":"Biochemical and biophysical research communications"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"origin":[{"publisherPlace":"[Amsterdam] ; Orlando, Fla.","dateIssuedKey":"1959","publisher":"Elsevier B.V. ; Academic Press","dateIssuedDisp":"1959-"}],"pubHistory":["1.1959 -"],"disp":"Proximity mapping of human separase by the BioID approachBiochemical and biophysical research communications","part":{"issue":"2","extent":"7","text":"478(2016), 2, Seite 656-662","year":"2016","pages":"656-662","volume":"478"}}],"recId":"1726783871","id":{"doi":["10.1016/j.bbrc.2016.08.002"],"eki":["1726783871"]},"language":["eng"],"physDesc":[{"extent":"7 S."}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 11.08.2020"],"person":[{"given":"Fikret Gürkan","role":"aut","roleDisplay":"VerfasserIn","display":"Agircan, Fikret Gürkan","family":"Agircan"},{"given":"Shoji","role":"aut","roleDisplay":"VerfasserIn","family":"Hata","display":"Hata, Shoji"},{"roleDisplay":"VerfasserIn","display":"Nussbaum-Krammer, Carmen","family":"Nussbaum-Krammer","given":"Carmen","role":"aut"},{"roleDisplay":"VerfasserIn","family":"Atorino","display":"Atorino, Enrico","given":"Enrico","role":"aut"},{"family":"Schiebel","display":"Schiebel, Elmar","roleDisplay":"VerfasserIn","role":"aut","given":"Elmar"}],"title":[{"title":"Proximity mapping of human separase by the BioID approach","title_sort":"Proximity mapping of human separase by the BioID approach"}]} 
SRT |a AGIRCANFIKPROXIMITYM3201